FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 30h_77.34100000005a21.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 30h_77.34100000005a21.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences510702
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGCGC406.1624346E-428.12420510
GCACACG450.001225823424.99929427
CCCTTAA500.002256840222.5103841
GTATCAA7200.021.8850941
TGCTCGC654.231799E-420.77067614
GCTCGCA550.003932003420.45596915
GAGGGCG807.5541386E-519.6869439
AGACGAT600.00650672118.749477
GCCCACG851.2021359E-418.53070318
GGTATCA4050.018.3417931
CATCTCC17750.018.12624745
TATGCCG750.001109285617.9994943
TAAGGTT750.001109285617.999494
CCCACGA1053.107706E-517.14237219
TTAACCA1053.107706E-517.1423724
CGGGTAC1205.1583484E-616.87617517
CTCCGAG3600.016.87452345
TAACACC800.001709411616.8745234
GCTACAC1205.1631596E-616.8745213
AACGCAG9350.016.603816