FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 30h_76.34100000005a14.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 30h_76.34100000005a14.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences239737
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCCGT552.730303E-936.817544
TGCCGTC606.375558E-933.75641645
AATCTCG501.9732652E-631.49941636
CTCGTAT651.3940735E-831.15326939
TCGTATG702.8667273E-828.92803640
ATCTCGT755.601214E-826.999537
TATGCCG801.04671926E-725.3120343
CCCACGA801.04671926E-725.3120319
TATTGGA450.001222367725.0047512
GAACCAC450.001223855924.99953828
ACAATGT651.5174415E-524.230328
GCCCACG851.8809988E-723.82308818
CACGAGA851.8809988E-723.82308821
TCTCGTA851.8809988E-723.82308838
CCACGAG851.8809988E-723.82308820
ACGAGAC903.265086E-722.49958422
TCTATGT500.00225966722.4995843
CGAGCCC955.4951306E-721.31539315
GGCTAGG854.9875707E-621.1849161
CGAGACT855.005644E-621.17607723
Produced by FastQC (version 0.11.2)