FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 30h_74.341000000059f9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 30h_74.341000000059f9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences226090
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGAGC300.005139604729.99882122
GTATCAA5750.023.8739091
ACGGGGG602.459667E-422.49911716
AGTACTC602.459667E-422.4991175
CTCCGAG1750.020.5706245
GGTATCA2850.019.740431
CCTTTAG706.951225E-419.2892231
GTGTAGG1051.4325997E-619.2892231
TGGACTC706.9615874E-419.28495610
AACGCAG6650.019.2849566
CAACGCA6800.019.1904225
ACGCAGA6750.018.9992527
CGCAGAG7000.018.6421248
TATCAAC7150.018.251032
TCAACGC7250.017.9992924
AGAGTAC7400.017.63444111
ACGGGGT901.8578836E-417.49931316
TACGGGG2450.017.44829415
ATCAACG7550.017.2840883
GAGTACG7300.017.25959612
Produced by FastQC (version 0.11.2)