FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 30h_71.341000000059c2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 30h_71.341000000059c2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences525119
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCAACTGTATCTCGTATGCCGTC23610.44961237357627504No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCTCG3650.040.06730336
CTCGTAT3850.039.15468239
TCGTATG3800.039.0777940
TATGCCG3750.038.99884843
ACGAGAC3900.038.65270222
TCTCGTA3950.038.16342538
TGCCGTC3800.037.8972245
ATGCCGT3850.037.4014944
CGTATGC4100.036.76720441
AACTGTA3750.036.5989231
ACTGTAT3800.036.1173532
AATAGGG250.002106375335.998933
CCCACGA4250.035.46953619
CGAGACT4000.035.4364523
CGAGCCC4400.035.28304715
CACGAGA4350.034.65414421
GTATCTC4200.034.284735
CCGAGCC4500.033.99899714
CCACGAG4450.033.87540420
ACTCAAC4150.033.61666527
Produced by FastQC (version 0.11.2)