FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 30h_70.341000000059b5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 30h_70.341000000059b5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences531322
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGCGGT250.002106134335.9998518
TGTGCGA352.818419E-432.1427210
TTGCCCG300.005144901629.99987410
GCCCACG1250.028.7998818
TATCTCG1100.028.63624436
TATGCCG1150.027.39119143
TCGTATG1150.027.39119140
ACGAGAC1150.027.39119122
TGCCGTC1150.027.39119145
CTCGTAT1200.026.2498939
ATGCCGT1301.8189894E-1224.23066944
CGAGACT1152.6375346E-1023.47816323
GGCGTGT500.002263074522.4999078
CACGAGA1459.094947E-1221.72404721
CCCACGA1459.094947E-1221.72404719
CGAGCCC1459.094947E-1221.72404715
GTATCAA8250.021.0038661
GGTATCA4650.020.8102821
GAGTAAT654.228842E-420.7730521
CAATAGT654.2342185E-420.7691444
Produced by FastQC (version 0.11.2)