FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 30h_69.341000000059a9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 30h_69.341000000059a9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences521030
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCCTAT901.184344E-825.0070381
CGACGCG651.5217494E-524.23061617
GTCCTAT702.693464E-522.5063361
GATATTG500.002263044722.4998577
CGGTAGT500.002263044722.49985714
ACGCGCA702.6994638E-522.49985719
AACCGTC754.5966575E-520.99986634
ACGCCTA754.5966575E-520.99986621
CGCCTAA754.5966575E-520.99986622
ACCGTCT754.5966575E-520.99986635
CGCAATC754.5966575E-520.99986622
CAATCGG754.5966575E-520.99986624
GTCCTAA654.2259067E-420.7750821
CTATTCC1301.2223609E-920.7690984
CGTTGTA550.0039338420.4544142
AAACGGG550.0039338420.4544145
GCGAGAT807.553242E-519.68737440
CGAGATA807.553242E-519.68737441
GACGCGC807.553242E-519.68737418
GGTCTAC1403.0649971E-919.2911451
Produced by FastQC (version 0.11.2)