FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 30h_68.3410000000599c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 30h_68.3410000000599c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences274443
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCAACTGTATCTCGTATGCCGTC4590.1672478438145626No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTGCGA250.002104362235.9998510
TGGACCG352.8148878E-432.1427275
TTAAGAT352.8148878E-432.1427273
CTGTGCG352.8148878E-432.1427279
TGCCGTC1000.031.4998745
GTGTAGG602.4038673E-730.0053461
CTATTAA300.00513603330.0053461
CGACCCG300.00514061329.9998785
CTCGTAT1001.8189894E-1229.2498839
TATGCCG1100.028.63624643
TCTCGTA1100.028.63624638
CGTATGC1150.027.39119341
ATGCCGT1150.027.39119344
TATCTCG1004.5474735E-1126.9998936
ATCTCGT1105.456968E-1226.59080137
TCGTATG1200.026.24989340
CCCACGA1300.025.96143319
GCCCACG1251.8189894E-1225.199918
GAGTCCC551.3645369E-424.5453557
ACGAGAC1301.8189894E-1224.2306722
Produced by FastQC (version 0.11.2)