FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 30h_67.3410000000598f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 30h_67.3410000000598f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences433071
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGACC300.005144725629.9988463
AATACTA450.001224770425.0019255
TAAGGAT450.001225595424.9990374
ACCGTGT751.6924405E-624.0018488
GTATCAA7050.022.9804921
ACGGGGC1003.6281563E-822.50173216
CGGGGGT500.00226285522.49913417
GGTATCA3700.021.2854211
CTATTGC654.230158E-420.7708281
TGTCGGG550.00392825620.4584810
CCCGGGG550.00393088520.456128
GTATTGG807.544643E-519.6890161
GGCGTTT600.006501205718.75144412
TACGGGG3500.018.00138715
TAAGGTG750.00110813218.0013875
ATAACTT750.00110813218.0013875
CTGCGGT800.001708961416.8743522
CAACGCA9000.016.751295
AACGCAG9000.016.751296
CTCCGAG2700.016.66602545
Produced by FastQC (version 0.11.2)