##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename H23NVBCXX l02n01 30h_64.34100000005959.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1053 Sequences flagged as poor quality 0 Sequence length 51 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.86039886039886 38.0 32.0 38.0 27.0 38.0 2 34.95536562203229 38.0 32.0 38.0 32.0 38.0 3 35.021842355175686 38.0 32.0 38.0 32.0 38.0 4 34.99240265906933 38.0 32.0 38.0 32.0 38.0 5 35.14339981006648 38.0 32.0 38.0 32.0 38.0 6 37.05698005698006 38.0 38.0 40.0 32.0 40.0 7 35.95821462488129 38.0 38.0 40.0 32.0 40.0 8 36.054131054131055 38.0 38.0 40.0 32.0 40.0 9 36.39981006647673 38.0 38.0 40.0 32.0 40.0 10 36.282051282051285 38.0 38.0 40.0 32.0 40.0 11 36.41120607787274 38.0 38.0 40.0 32.0 40.0 12 35.88129154795821 38.0 38.0 40.0 32.0 40.0 13 36.400759734093064 38.0 38.0 40.0 32.0 40.0 14 35.99430199430199 38.0 38.0 40.0 32.0 40.0 15 35.54605887939221 38.0 32.0 40.0 27.0 40.0 16 35.31433998100665 38.0 32.0 40.0 27.0 40.0 17 34.65052231718899 38.0 32.0 38.0 27.0 40.0 18 34.96011396011396 38.0 32.0 38.0 27.0 40.0 19 35.15859449192782 38.0 32.0 40.0 27.0 40.0 20 35.26115859449193 38.0 32.0 40.0 27.0 40.0 21 35.92022792022792 38.0 38.0 40.0 27.0 40.0 22 35.934472934472936 38.0 38.0 40.0 27.0 40.0 23 35.66096866096866 38.0 38.0 40.0 27.0 40.0 24 35.547008547008545 38.0 32.0 40.0 27.0 40.0 25 35.12630579297246 38.0 32.0 40.0 27.0 40.0 26 34.88698955365622 38.0 32.0 40.0 27.0 40.0 27 35.32383665716999 38.0 32.0 40.0 27.0 40.0 28 35.12535612535613 38.0 32.0 40.0 27.0 40.0 29 35.25261158594492 38.0 32.0 40.0 27.0 40.0 30 35.110161443494775 38.0 32.0 40.0 27.0 40.0 31 34.82336182336182 38.0 32.0 38.0 27.0 40.0 32 34.95441595441596 38.0 32.0 38.0 27.0 40.0 33 34.977207977207975 38.0 32.0 40.0 27.0 40.0 34 35.52516619183286 38.0 38.0 40.0 27.0 40.0 35 35.50522317188984 38.0 38.0 40.0 27.0 40.0 36 35.38081671415005 38.0 32.0 40.0 27.0 40.0 37 34.81006647673314 38.0 32.0 40.0 27.0 40.0 38 35.21557454890788 38.0 32.0 40.0 27.0 40.0 39 34.45679012345679 38.0 32.0 38.0 13.0 40.0 40 35.10636277302944 38.0 32.0 40.0 27.0 40.0 41 35.01234567901235 38.0 32.0 40.0 27.0 40.0 42 34.65906932573599 38.0 32.0 38.0 27.0 40.0 43 35.396011396011396 38.0 32.0 40.0 27.0 40.0 44 35.01139601139601 38.0 32.0 38.0 27.0 40.0 45 35.07502374169041 38.0 32.0 40.0 27.0 40.0 46 34.028490028490026 38.0 32.0 38.0 13.0 40.0 47 34.10636277302944 38.0 32.0 38.0 13.0 40.0 48 34.14434947768281 38.0 32.0 38.0 13.0 40.0 49 33.598290598290596 38.0 32.0 38.0 13.0 40.0 50 34.1718898385565 38.0 32.0 38.0 27.0 40.0 51 33.39126305792973 38.0 32.0 38.0 13.0 40.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 3.4344262295081975 1101 2 4.44535519125683 1101 3 3.7267759562841505 1101 4 3.1584699453551934 1101 5 -3.2131147540983633 1101 6 0.7431693989070993 1101 7 3.7349726775956285 1101 8 1.322404371584696 1101 9 1.7896174863387984 1101 10 1.6202185792349724 1101 11 1.784153005464475 1101 12 2.092896174863384 1101 13 1.9945355191256908 1101 14 1.6338797814207595 1101 15 3.7868852459016367 1101 16 3.4562841530054698 1101 17 4.806010928961747 1101 18 -2.669398907103826 1101 19 -3.330601092896181 1101 20 -2.34699453551913 1101 21 -22.032786885245905 1101 22 3.4207650273224104 1101 23 1.6967213114754074 1101 24 3.008196721311478 1101 25 -3.5601092896174933 1101 26 3.683060109289613 1101 27 3.0273224043715814 1101 28 3.0874316939890676 1101 29 1.9562841530054698 1101 30 -2.6639344262295097 1101 31 2.639344262295083 1101 32 -1.9508196721311535 1101 33 2.4808743169398824 1101 34 3.0601092896174933 1101 35 3.0109289617486326 1101 36 3.076502732240442 1101 37 3.3278688524590194 1101 38 3.6803278688524586 1101 39 3.647540983606561 1101 40 3.3633879781420717 1101 41 3.4590163934426243 1101 42 6.5573770491803245 1101 43 4.674863387978142 1101 44 6.248633879781416 1101 45 3.355191256830608 1101 46 5.295081967213115 1101 47 6.125683060109296 1101 48 4.34699453551913 1101 49 4.360655737704917 1101 50 4.37158469945355 1101 51 5.16393442622951 2201 1 0.43442622950819754 2201 2 -8.05464480874317 2201 3 3.7267759562841505 2201 4 -9.341530054644807 2201 5 -9.713114754098363 2201 6 -3.7568306010929007 2201 7 -10.765027322404372 2201 8 -0.677595628415304 2201 9 -0.21038251366120164 2201 10 2.6202185792349724 2201 11 2.784153005464475 2201 12 3.092896174863384 2201 13 2.994535519125691 2201 14 -0.3661202185792405 2201 15 4.786885245901637 2201 16 1.4562841530054698 2201 17 0.8060109289617472 2201 18 4.330601092896174 2201 19 0.6693989071038189 2201 20 4.65300546448087 2201 21 -0.032786885245904784 2201 22 -2.0792349726775896 2201 23 -3.8032786885245926 2201 24 0.008196721311477972 2201 25 2.4398907103825067 2201 26 1.6830601092896131 2201 27 -1.4726775956284186 2201 28 -8.412568306010932 2201 29 -9.54371584699453 2201 30 -9.16393442622951 2201 31 -1.860655737704917 2201 32 5.0491803278688465 2201 33 3.4808743169398824 2201 34 -8.439890710382507 2201 35 -8.489071038251367 2201 36 -8.423497267759558 2201 37 -2.1721311475409806 2201 38 -7.8196721311475414 2201 39 -0.8524590163934391 2201 40 1.3633879781420717 2201 41 -8.040983606557376 2201 42 -6.9426229508196755 2201 43 3.6748633879781423 2201 44 2.2486338797814156 2201 45 1.355191256830608 2201 46 4.295081967213115 2201 47 4.125683060109296 2201 48 -1.15300546448087 2201 49 1.360655737704917 2201 50 4.37158469945355 2201 51 2.1639344262295097 1102 1 3.4344262295081975 1102 2 -8.05464480874317 1102 3 -1.7732240437158495 1102 4 3.1584699453551934 1102 5 -0.21311475409836333 1102 6 0.7431693989070993 1102 7 1.7349726775956285 1102 8 1.322404371584696 1102 9 2.7896174863387984 1102 10 2.6202185792349724 1102 11 2.784153005464475 1102 12 0.09289617486338386 1102 13 1.9945355191256908 1102 14 1.6338797814207595 1102 15 -2.2131147540983633 1102 16 -2.0437158469945302 1102 17 2.806010928961747 1102 18 0.330601092896174 1102 19 2.669398907103819 1102 20 3.65300546448087 1102 21 -0.032786885245904784 1102 22 4.42076502732241 1102 23 -0.3032786885245926 1102 24 -8.491803278688522 1102 25 -9.060109289617493 1102 26 -1.8169398907103869 1102 27 -9.472677595628419 1102 28 0.08743169398906758 1102 29 1.9562841530054698 1102 30 4.33606557377049 1102 31 2.639344262295083 1102 32 5.0491803278688465 1102 33 3.4808743169398824 1102 34 1.0601092896174933 1102 35 4.010928961748633 1102 36 4.076502732240442 1102 37 0.3278688524590194 1102 38 0.6803278688524586 1102 39 1.6475409836065609 1102 40 -8.136612021857928 1102 41 -9.040983606557376 1102 42 1.5573770491803245 1102 43 -1.8251366120218577 1102 44 5.248633879781416 1102 45 -9.144808743169392 1102 46 -12.204918032786885 1102 47 -1.3743169398907042 1102 48 -8.15300546448087 1102 49 -8.139344262295083 1102 50 -1.1284153005464503 1102 51 2.1639344262295097 2202 1 3.4344262295081975 2202 2 -1.55464480874317 2202 3 3.7267759562841505 2202 4 3.1584699453551934 2202 5 -0.21311475409836333 2202 6 2.7431693989070993 2202 7 2.7349726775956285 2202 8 2.322404371584696 2202 9 1.7896174863387984 2202 10 2.6202185792349724 2202 11 3.784153005464475 2202 12 4.092896174863384 2202 13 3.994535519125691 2202 14 3.6338797814207595 2202 15 5.786885245901637 2202 16 4.45628415300547 2202 17 3.806010928961747 2202 18 3.330601092896174 2202 19 3.669398907103819 2202 20 4.65300546448087 2202 21 3.967213114754095 2202 22 5.42076502732241 2202 23 2.6967213114754074 2202 24 4.008196721311478 2202 25 4.439890710382507 2202 26 3.683060109289613 2202 27 4.027322404371581 2202 28 3.0874316939890676 2202 29 2.9562841530054698 2202 30 4.33606557377049 2202 31 3.639344262295083 2202 32 6.0491803278688465 2202 33 4.480874316939882 2202 34 3.0601092896174933 2202 35 4.010928961748633 2202 36 3.076502732240442 2202 37 4.327868852459019 2202 38 4.6803278688524586 2202 39 5.647540983606561 2202 40 5.363387978142072 2202 41 4.459016393442624 2202 42 6.5573770491803245 2202 43 4.674863387978142 2202 44 6.248633879781416 2202 45 4.355191256830608 2202 46 4.295081967213115 2202 47 5.125683060109296 2202 48 5.34699453551913 2202 49 5.360655737704917 2202 50 4.37158469945355 2202 51 5.16393442622951 2203 1 0.43442622950819754 2203 2 4.44535519125683 2203 3 -2.2732240437158495 2203 4 0.15846994535519343 2203 5 2.7868852459016367 2203 6 2.7431693989070993 2203 7 1.7349726775956285 2203 8 -4.177595628415304 2203 9 1.7896174863387984 2203 10 -1.3797814207650276 2203 11 -1.215846994535525 2203 12 3.092896174863384 2203 13 2.994535519125691 2203 14 1.6338797814207595 2203 15 4.786885245901637 2203 16 4.45628415300547 2203 17 -8.693989071038253 2203 18 4.330601092896174 2203 19 -0.3306010928961811 2203 20 3.65300546448087 2203 21 -0.032786885245904784 2203 22 3.4207650273224104 2203 23 -1.3032786885245926 2203 24 3.008196721311478 2203 25 -3.5601092896174933 2203 26 0.6830601092896131 2203 27 0.0273224043715814 2203 28 0.08743169398906758 2203 29 -1.0437158469945302 2203 30 -1.1639344262295097 2203 31 -5.860655737704917 2203 32 1.0491803278688465 2203 33 -0.5191256830601176 2203 34 3.0601092896174933 2203 35 0.01092896174863256 2203 36 0.07650273224044213 2203 37 1.3278688524590194 2203 38 1.6803278688524586 2203 39 0.6475409836065609 2203 40 3.3633879781420717 2203 41 3.4590163934426243 2203 42 4.5573770491803245 2203 43 2.6748633879781423 2203 44 4.248633879781416 2203 45 3.355191256830608 2203 46 0.29508196721311464 2203 47 -4.374316939890704 2203 48 -1.15300546448087 2203 49 -4.139344262295083 2203 50 4.37158469945355 2203 51 2.1639344262295097 1103 1 1.4344262295081975 1103 2 4.44535519125683 1103 3 -0.2732240437158495 1103 4 1.1584699453551934 1103 5 2.7868852459016367 1103 6 0.7431693989070993 1103 7 -0.26502732240437155 1103 8 2.6557377049180317 1103 9 1.122950819672134 1103 10 2.953551912568308 1103 11 0.45081967213113927 1103 12 2.759562841530048 1103 13 2.661202185792355 1103 14 0.9672131147540952 1103 15 5.120218579234972 1103 16 4.122950819672134 1103 17 4.139344262295083 1103 18 1.330601092896174 1103 19 2.0027322404371546 1103 20 4.986338797814206 1103 21 4.300546448087431 1103 22 4.754098360655746 1103 23 3.6967213114754074 1103 24 3.6748633879781423 1103 25 4.439890710382507 1103 26 5.016393442622949 1103 27 3.6939890710382457 1103 28 5.087431693989068 1103 29 2.622950819672134 1103 30 4.669398907103826 1103 31 1.3060109289617472 1103 32 4.715846994535511 1103 33 2.4808743169398824 1103 34 1.7267759562841576 1103 35 4.344262295081968 1103 36 4.409836065573778 1103 37 4.661202185792355 1103 38 5.6803278688524586 1103 39 4.980874316939897 1103 40 4.030054644808736 1103 41 4.79234972677596 1103 42 3.89071038251366 1103 43 2.008196721311478 1103 44 5.581967213114751 1103 45 4.021857923497272 1103 46 2.6284153005464503 1103 47 5.4590163934426315 1103 48 5.013661202185794 1103 49 5.027322404371581 1103 50 5.704918032786885 1103 51 7.16393442622951 2204 1 -2.5655737704918025 2204 2 4.44535519125683 2204 3 3.7267759562841505 2204 4 -2.8415300546448066 2204 5 2.7868852459016367 2204 6 0.7431693989070993 2204 7 1.7349726775956285 2204 8 -4.677595628415304 2204 9 1.7896174863387984 2204 10 -4.379781420765028 2204 11 1.784153005464475 2204 12 4.092896174863384 2204 13 3.994535519125691 2204 14 1.6338797814207595 2204 15 3.7868852459016367 2204 16 3.4562841530054698 2204 17 -8.193989071038253 2204 18 -2.669398907103826 2204 19 2.669398907103819 2204 20 3.65300546448087 2204 21 2.967213114754095 2204 22 -21.57923497267759 2204 23 1.6967213114754074 2204 24 -2.991803278688522 2204 25 -3.5601092896174933 2204 26 -2.316939890710387 2204 27 -21.97267759562842 2204 28 -2.9125683060109324 2204 29 -4.04371584699453 2204 30 -21.66393442622951 2204 31 -3.360655737704917 2204 32 -6.9508196721311535 2204 33 2.4808743169398824 2204 34 3.0601092896174933 2204 35 3.0109289617486326 2204 36 3.076502732240442 2204 37 3.3278688524590194 2204 38 3.6803278688524586 2204 39 3.647540983606561 2204 40 5.363387978142072 2204 41 5.459016393442624 2204 42 4.5573770491803245 2204 43 2.6748633879781423 2204 44 4.248633879781416 2204 45 3.355191256830608 2204 46 3.2950819672131146 2204 47 -1.8743169398907042 2204 48 -1.65300546448087 2204 49 -6.639344262295083 2204 50 -6.62841530054645 2204 51 -0.8360655737704903 2205 1 -2.5655737704918025 2205 2 4.44535519125683 2205 3 -2.2732240437158495 2205 4 -2.8415300546448066 2205 5 2.7868852459016367 2205 6 0.7431693989070993 2205 7 1.7349726775956285 2205 8 1.322404371584696 2205 9 1.7896174863387984 2205 10 3.6202185792349724 2205 11 3.784153005464475 2205 12 2.092896174863384 2205 13 1.9945355191256908 2205 14 1.6338797814207595 2205 15 -2.2131147540983633 2205 16 -7.54371584699453 2205 17 -3.193989071038253 2205 18 3.330601092896174 2205 19 2.669398907103819 2205 20 3.65300546448087 2205 21 4.967213114754095 2205 22 3.4207650273224104 2205 23 1.6967213114754074 2205 24 5.008196721311478 2205 25 4.439890710382507 2205 26 3.683060109289613 2205 27 -2.9726775956284186 2205 28 3.0874316939890676 2205 29 1.9562841530054698 2205 30 -2.6639344262295097 2205 31 -8.360655737704917 2205 32 -6.9508196721311535 2205 33 2.4808743169398824 2205 34 -2.9398907103825067 2205 35 3.0109289617486326 2205 36 3.076502732240442 2205 37 -2.6721311475409806 2205 38 3.6803278688524586 2205 39 3.647540983606561 2205 40 -21.63661202185793 2205 41 -2.5409836065573757 2205 42 4.5573770491803245 2205 43 2.6748633879781423 2205 44 -6.751366120218584 2205 45 3.355191256830608 2205 46 3.2950819672131146 2205 47 4.125683060109296 2205 48 4.34699453551913 2205 49 4.360655737704917 2205 50 6.37158469945355 2205 51 7.16393442622951 1216 1 1.4344262295081975 1216 2 0.4453551912568301 1216 3 -4.606557377049182 1216 4 -0.8415300546448066 1216 5 2.7868852459016367 1216 6 0.7431693989070993 1216 7 -0.26502732240437155 1216 8 1.322404371584696 1216 9 -8.543715846994534 1216 10 -1.7131147540983633 1216 11 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-7.33606557377049 1214 1 3.4344262295081975 1214 2 -1.55464480874317 1214 3 3.7267759562841505 1214 4 3.1584699453551934 1214 5 -3.2131147540983633 1214 6 0.7431693989070993 1214 7 1.7349726775956285 1214 8 1.322404371584696 1214 9 3.7896174863387984 1214 10 3.6202185792349724 1214 11 3.784153005464475 1214 12 4.092896174863384 1214 13 3.994535519125691 1214 14 1.6338797814207595 1214 15 3.7868852459016367 1214 16 5.45628415300547 1214 17 4.806010928961747 1214 18 3.330601092896174 1214 19 4.669398907103819 1214 20 3.65300546448087 1214 21 2.967213114754095 1214 22 -2.5792349726775896 1214 23 -4.303278688524593 1214 24 -2.991803278688522 1214 25 2.4398907103825067 1214 26 3.683060109289613 1214 27 3.0273224043715814 1214 28 3.0874316939890676 1214 29 1.9562841530054698 1214 30 3.3360655737704903 1214 31 4.639344262295083 1214 32 4.0491803278688465 1214 33 4.480874316939882 1214 34 3.0601092896174933 1214 35 5.010928961748633 1214 36 3.076502732240442 1214 37 3.3278688524590194 1214 38 3.6803278688524586 1214 39 -2.352459016393439 1214 40 3.3633879781420717 1214 41 3.4590163934426243 1214 42 -1.4426229508196755 1214 43 2.6748633879781423 1214 44 4.248633879781416 1214 45 3.355191256830608 1214 46 3.2950819672131146 1214 47 4.125683060109296 1214 48 4.34699453551913 1214 49 4.360655737704917 1214 50 6.37158469945355 1214 51 -0.8360655737704903 1212 1 -2.5655737704918025 1212 2 -1.55464480874317 1212 3 3.7267759562841505 1212 4 3.1584699453551934 1212 5 -3.2131147540983633 1212 6 0.7431693989070993 1212 7 1.7349726775956285 1212 8 3.322404371584696 1212 9 1.7896174863387984 1212 10 1.6202185792349724 1212 11 1.784153005464475 1212 12 4.092896174863384 1212 13 1.9945355191256908 1212 14 1.6338797814207595 1212 15 3.7868852459016367 1212 16 -7.54371584699453 1212 17 2.806010928961747 1212 18 3.330601092896174 1212 19 2.669398907103819 1212 20 3.65300546448087 1212 21 4.967213114754095 1212 22 5.42076502732241 1212 23 3.6967213114754074 1212 24 3.008196721311478 1212 25 4.439890710382507 1212 26 3.683060109289613 1212 27 3.0273224043715814 1212 28 3.0874316939890676 1212 29 1.9562841530054698 1212 30 3.3360655737704903 1212 31 2.639344262295083 1212 32 4.0491803278688465 1212 33 2.4808743169398824 1212 34 3.0601092896174933 1212 35 3.0109289617486326 1212 36 3.076502732240442 1212 37 3.3278688524590194 1212 38 5.6803278688524586 1212 39 5.647540983606561 1212 40 5.363387978142072 1212 41 3.4590163934426243 1212 42 4.5573770491803245 1212 43 2.6748633879781423 1212 44 6.248633879781416 1212 45 3.355191256830608 1212 46 3.2950819672131146 1212 47 6.125683060109296 1212 48 4.34699453551913 1212 49 4.360655737704917 1212 50 4.37158469945355 1212 51 5.16393442622951 1213 1 -2.5655737704918025 1213 2 -1.55464480874317 1213 3 -2.2732240437158495 1213 4 -2.8415300546448066 1213 5 -3.2131147540983633 1213 6 0.7431693989070993 1213 7 -4.2650273224043715 1213 8 -4.677595628415304 1213 9 1.7896174863387984 1213 10 -9.379781420765028 1213 11 -4.215846994535525 1213 12 2.092896174863384 1213 13 1.9945355191256908 1213 14 1.6338797814207595 1213 15 -2.2131147540983633 1213 16 3.4562841530054698 1213 17 4.806010928961747 1213 18 3.330601092896174 1213 19 2.669398907103819 1213 20 -21.34699453551913 1213 21 -3.032786885245905 1213 22 3.4207650273224104 1213 23 1.6967213114754074 1213 24 3.008196721311478 1213 25 2.4398907103825067 1213 26 -2.316939890710387 1213 27 -7.972677595628419 1213 28 3.0874316939890676 1213 29 1.9562841530054698 1213 30 -2.6639344262295097 1213 31 2.639344262295083 1213 32 -6.9508196721311535 1213 33 -8.519125683060118 1213 34 -2.9398907103825067 1213 35 -2.9890710382513674 1213 36 3.076502732240442 1213 37 3.3278688524590194 1213 38 -21.31967213114754 1213 39 -2.352459016393439 1213 40 3.3633879781420717 1213 41 -2.5409836065573757 1213 42 -1.4426229508196755 1213 43 2.6748633879781423 1213 44 -1.7513661202185844 1213 45 -7.644808743169392 1213 46 -2.7049180327868854 1213 47 -1.8743169398907042 1213 48 -1.65300546448087 1213 49 4.360655737704917 1213 50 4.37158469945355 1213 51 -0.8360655737704903 >>END_MODULE >>Per sequence quality scores pass #Quality Count 23 1.0 24 8.0 25 9.0 26 16.0 27 24.0 28 38.0 29 40.0 30 41.0 31 55.0 32 50.0 33 71.0 34 62.0 35 80.0 36 105.0 37 135.0 38 166.0 39 152.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 15.779467680608365 8.26996197718631 8.935361216730039 67.01520912547528 2 6.552706552706552 10.351377018043685 72.4596391263058 10.63627730294397 3 8.452041785375117 14.4349477682811 67.1415004748338 9.971509971509972 4 6.077872744539412 61.72839506172839 17.28395061728395 14.909781576448244 5 5.318138651471985 19.943019943019944 68.28110161443495 6.457739791073125 6 13.865147198480532 70.56030389363723 7.122507122507122 8.452041785375117 7 10.826210826210826 19.278252611585945 9.781576448243115 60.11396011396012 8 11.016144349477683 69.61063627730294 7.88224121557455 11.490978157644824 9 9.876543209876543 10.921177587844255 9.49667616334283 69.70560303893637 10 5.982905982905983 64.86229819563152 17.94871794871795 11.20607787274454 11 14.82889733840304 11.406844106463879 63.21292775665399 10.551330798479087 12 8.547008547008547 11.77587844254511 18.708452041785375 60.96866096866097 13 13.200379867046532 9.306742640075974 63.53276353276354 13.96011396011396 14 9.781576448243115 10.256410256410255 13.96011396011396 66.00189933523268 15 12.250712250712251 13.105413105413104 10.826210826210826 63.817663817663814 16 61.91832858499525 15.7644824311491 11.396011396011396 10.921177587844255 17 15.859449192782527 64.00759734093067 10.0664767331434 10.0664767331434 18 62.86799620132953 12.250712250712251 12.82051282051282 12.060778727445394 19 12.155745489078823 11.77587844254511 13.675213675213676 62.39316239316239 20 11.11111111111111 10.161443494776828 12.440645773979107 66.28679962013295 21 9.116809116809117 17.188983855650523 12.440645773979107 61.25356125356125 22 9.591642924976258 62.96296296296296 12.155745489078823 15.289648622981955 23 11.965811965811966 14.909781576448244 12.060778727445394 61.0636277302944 24 60.20892687559354 16.904083570750235 13.200379867046532 9.686609686609685 25 14.4349477682811 62.48812915479582 13.010446343779677 10.0664767331434 26 61.25356125356125 17.758784425451093 12.725546058879392 8.262108262108262 27 11.301044634377968 63.24786324786324 12.345679012345679 13.105413105413104 28 10.256410256410255 15.57454890788224 13.675213675213676 60.49382716049383 29 22.79202279202279 44.53941120607787 20.7977207977208 11.870845204178536 30 38.93637226970561 32.85849952516619 17.28395061728395 10.921177587844255 31 18.61348528015195 42.83000949667616 17.568850902184234 20.98765432098765 32 17.47388414055081 25.071225071225072 45.77397910731244 11.68091168091168 33 11.20607787274454 47.483380816714146 24.976258309591643 16.334283000949668 34 13.770180436847104 17.188983855650523 49.572649572649574 19.468186134852804 35 12.440645773979107 48.907882241215574 18.51851851851852 20.1329534662868 36 8.167141500474834 47.768281101614434 32.668566001899336 11.396011396011396 37 8.167141500474834 62.86799620132953 18.328584995251664 10.63627730294397 38 8.831908831908832 12.535612535612536 64.48243114909782 14.150047483380817 39 8.262108262108262 11.11111111111111 19.56315289648623 61.0636277302944 40 9.781576448243115 9.971509971509972 65.71699905033238 14.529914529914532 41 12.82051282051282 10.161443494776828 17.094017094017094 59.92402659069326 42 58.21462488129154 11.20607787274454 17.94871794871795 12.630579297245964 43 10.256410256410255 14.4349477682811 64.29249762583096 11.016144349477683 44 8.357075023741691 60.96866096866097 19.943019943019944 10.731244064577398 45 11.490978157644824 11.965811965811966 65.05223171889838 11.490978157644824 46 58.0246913580247 11.301044634377968 15.954415954415953 14.719848053181387 47 9.591642924976258 12.440645773979107 14.339981006647673 63.627730294396954 48 10.826210826210826 12.060778727445394 13.200379867046532 63.91263057929725 49 57.92972459639126 10.541310541310542 19.08831908831909 12.440645773979107 50 10.0664767331434 11.396011396011396 64.29249762583096 14.245014245014245 51 6.457739791073125 10.921177587844255 17.94871794871795 64.67236467236467 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.5 12 1.0 13 1.0 14 1.0 15 1.5 16 2.0 17 2.0 18 2.0 19 2.0 20 2.0 21 2.5 22 3.0 23 2.0 24 1.0 25 2.5 26 7.0 27 10.0 28 6.5 29 3.0 30 10.0 31 17.0 32 13.5 33 10.0 34 15.5 35 21.0 36 23.0 37 25.0 38 26.0 39 27.0 40 38.0 41 49.0 42 40.5 43 32.0 44 60.5 45 89.0 46 222.0 47 355.0 48 208.0 49 61.0 50 101.5 51 142.0 52 137.5 53 133.0 54 85.5 55 38.0 56 24.0 57 10.0 58 8.5 59 7.0 60 4.0 61 1.0 62 2.0 63 3.0 64 3.0 65 3.0 66 1.5 67 0.0 68 0.0 69 0.0 70 0.0 71 0.0 72 0.0 73 0.0 74 0.5 75 0.5 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0949667616334283 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0949667616334283 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 1053.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.187084520417855 #Duplication Level Percentage of deduplicated Percentage of total 1 95.17625231910947 48.717948717948715 2 2.411873840445269 2.4691358024691357 3 0.0 0.0 4 0.1855287569573284 0.3798670465337132 5 0.3710575139146568 0.949667616334283 6 0.3710575139146568 1.1396011396011396 7 0.0 0.0 8 0.0 0.0 9 0.0 0.0 >10 1.1131725417439702 12.630579297245964 >50 0.1855287569573284 6.932573599240266 >100 0.1855287569573284 26.780626780626783 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACAGATATAAATCTCGTATGCCGTC 282 26.780626780626783 No Hit CTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGTC 73 6.932573599240266 No Hit CTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGTC 36 3.418803418803419 No Hit CTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGTC 31 2.9439696106362776 No Hit CTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGTC 22 2.089268755935423 No Hit CTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGTC 18 1.7094017094017095 No Hit CTTATACACATCTCCGAGCCCACGAGACGAATTGCTATCTCGTATGCCGTC 16 1.519468186134853 RNA PCR Primer, Index 27 (95% over 21bp) CTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGTC 10 0.949667616334283 No Hit CTTATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGTC 6 0.5698005698005698 TruSeq Adapter, Index 12 (95% over 21bp) TTATACACATCTCCGAGCCCACGAGACAGATATAAATCTCGTATGCCGTCT 6 0.5698005698005698 No Hit TTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGTCT 5 0.4748338081671415 Illumina PCR Primer Index 11 (95% over 21bp) CTTATACACATCTCCGAGCCCACGAGACCATTCCGAATCTCGTATGCCGTC 5 0.4748338081671415 RNA PCR Primer, Index 46 (95% over 21bp) TTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGTCT 4 0.3798670465337132 No Hit TTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGTCT 2 0.1899335232668566 RNA PCR Primer, Index 27 (95% over 21bp) CTTATACACATCTCCGAGCCCACGAGACAGATATAAATATCGTATGCCGTC 2 0.1899335232668566 No Hit CTTATACACATCTCCGAGCCCACGAGACCAAGGCGAATCTCGTATGCCGTC 2 0.1899335232668566 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2 0.1899335232668566 No Hit CTTATACACATCTCCGAGCCCACGAGCAGATATAAATCTCGTATGCCGTCT 2 0.1899335232668566 No Hit TTATACACATCTCCGAGCCCACGAGACGAATTGCTATCTCGTATGCCGTCT 2 0.1899335232668566 RNA PCR Primer, Index 38 (95% over 21bp) CTTATACACATCTCCGAGCCCCACGAGACGACGCTATATCTCGTATGCCGT 2 0.1899335232668566 No Hit CTTATACACCATCTCCGAGCCCACGAGACAGATATAAATCTCGTATGCCGT 2 0.1899335232668566 No Hit CTTATACACATTCTCCGAGCCCACGAGACAGATATAAATCTCGTATGCCGT 2 0.1899335232668566 No Hit ACTTAGAGCTGCATTCCCAAACAACTCGACTCTTCGAAGGAACTTTACATA 2 0.1899335232668566 No Hit CTTATACACACTCCGAGCCCACGAGACAGATATAAATCTCGTATGCCGTCT 2 0.1899335232668566 No Hit CGGAGATGTGTATAAGAGACAGCTTATACACATCTCCGAGCCCACGAGACT 2 0.1899335232668566 No Hit GTACGGGAGTGAAGCGGCAAAAGCTCAAATTTGAAATCTGGCACTTTCAGT 2 0.1899335232668566 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0 0.0 0.0 2 0.0 0.0 0.0 3 0.0 0.0 0.0 4 0.0 0.0 0.0 5 0.0 0.0 0.0 6 0.0 0.0 0.0 7 0.0 0.0 0.0 8 0.0 0.0 0.0 9 0.0 0.0 0.0 10 0.0 0.0 0.0 11 0.0 0.0 0.0 12 0.0 0.0 0.0 13 0.0 0.0 0.0 14 0.0 0.0 0.0 15 0.0 0.0 0.0 16 0.0 0.0 0.0 17 0.0 0.0 0.0 18 0.0 0.0 0.0 19 0.0 0.0 0.0 20 0.0 0.0 0.0 21 0.0 0.0 0.0 22 0.0 0.0 0.0 23 0.0 0.0 0.0949667616334283 24 0.0 0.0 0.1899335232668566 25 0.0 0.0 0.1899335232668566 26 0.0 0.0 0.1899335232668566 27 0.0 0.0 0.1899335232668566 28 0.0 0.0 0.1899335232668566 29 0.0 0.0 0.1899335232668566 30 0.0 0.0 0.2849002849002849 31 0.0 0.0 0.7597340930674265 32 0.0 0.0 1.2345679012345678 33 0.0 0.0 1.7094017094017093 34 0.0 0.0 2.3741690408357075 35 0.0 0.0 2.849002849002849 36 0.0 0.0 3.5137701804368473 37 0.0 0.0 4.938271604938271 38 0.0 0.0 6.362773029439696 39 0.0 0.0 7.597340930674264 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TGCCGTC 40 7.9728125E-8 39.375 45 AATCTCG 35 2.128525E-6 38.57143 36 ACAGATA 35 2.128525E-6 38.57143 27 TAAATCT 35 2.128525E-6 38.57143 34 AAATCTC 35 2.128525E-6 38.57143 35 GATATAA 35 2.128525E-6 38.57143 30 GACAGAT 35 2.128525E-6 38.57143 26 TATAAAT 35 2.128525E-6 38.57143 32 ATAAATC 35 2.128525E-6 38.57143 33 CAGATAT 35 2.128525E-6 38.57143 28 CGAGACA 35 2.128525E-6 38.57143 23 AGACAGA 35 2.128525E-6 38.57143 25 AGATATA 35 2.128525E-6 38.57143 29 GAGACAG 35 2.128525E-6 38.57143 24 ATATAAA 35 2.128525E-6 38.57143 31 CTTATAC 50 7.1431714E-9 36.000004 1 AGCCCAC 45 2.0206062E-7 35.0 17 GTATGCC 45 2.0206062E-7 35.0 42 ATCTCGT 45 2.0206062E-7 35.0 37 ATCTCCG 45 2.0206062E-7 35.0 10 >>END_MODULE