FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 30h_64.34100000005959.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 30h_64.34100000005959.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1053
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGATATAAATCTCGTATGCCGTC28226.780626780626783No Hit
CTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGTC736.932573599240266No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGTC363.418803418803419No Hit
CTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGTC312.9439696106362776No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGTC222.089268755935423No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGTC181.7094017094017095No Hit
CTTATACACATCTCCGAGCCCACGAGACGAATTGCTATCTCGTATGCCGTC161.519468186134853RNA PCR Primer, Index 27 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGTC100.949667616334283No Hit
CTTATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGTC60.5698005698005698TruSeq Adapter, Index 12 (95% over 21bp)
TTATACACATCTCCGAGCCCACGAGACAGATATAAATCTCGTATGCCGTCT60.5698005698005698No Hit
TTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGTCT50.4748338081671415Illumina PCR Primer Index 11 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACCATTCCGAATCTCGTATGCCGTC50.4748338081671415RNA PCR Primer, Index 46 (95% over 21bp)
TTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGTCT40.3798670465337132No Hit
TTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGTCT20.1899335232668566RNA PCR Primer, Index 27 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACAGATATAAATATCGTATGCCGTC20.1899335232668566No Hit
CTTATACACATCTCCGAGCCCACGAGACCAAGGCGAATCTCGTATGCCGTC20.1899335232668566No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20.1899335232668566No Hit
CTTATACACATCTCCGAGCCCACGAGCAGATATAAATCTCGTATGCCGTCT20.1899335232668566No Hit
TTATACACATCTCCGAGCCCACGAGACGAATTGCTATCTCGTATGCCGTCT20.1899335232668566RNA PCR Primer, Index 38 (95% over 21bp)
CTTATACACATCTCCGAGCCCCACGAGACGACGCTATATCTCGTATGCCGT20.1899335232668566No Hit
CTTATACACCATCTCCGAGCCCACGAGACAGATATAAATCTCGTATGCCGT20.1899335232668566No Hit
CTTATACACATTCTCCGAGCCCACGAGACAGATATAAATCTCGTATGCCGT20.1899335232668566No Hit
ACTTAGAGCTGCATTCCCAAACAACTCGACTCTTCGAAGGAACTTTACATA20.1899335232668566No Hit
CTTATACACACTCCGAGCCCACGAGACAGATATAAATCTCGTATGCCGTCT20.1899335232668566No Hit
CGGAGATGTGTATAAGAGACAGCTTATACACATCTCCGAGCCCACGAGACT20.1899335232668566No Hit
GTACGGGAGTGAAGCGGCAAAAGCTCAAATTTGAAATCTGGCACTTTCAGT20.1899335232668566No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC407.9728125E-839.37545
AATCTCG352.128525E-638.5714336
ACAGATA352.128525E-638.5714327
TAAATCT352.128525E-638.5714334
AAATCTC352.128525E-638.5714335
GATATAA352.128525E-638.5714330
GACAGAT352.128525E-638.5714326
TATAAAT352.128525E-638.5714332
ATAAATC352.128525E-638.5714333
CAGATAT352.128525E-638.5714328
CGAGACA352.128525E-638.5714323
AGACAGA352.128525E-638.5714325
AGATATA352.128525E-638.5714329
GAGACAG352.128525E-638.5714324
ATATAAA352.128525E-638.5714331
CTTATAC507.1431714E-936.0000041
AGCCCAC452.0206062E-735.017
GTATGCC452.0206062E-735.042
ATCTCGT452.0206062E-735.037
ATCTCCG452.0206062E-735.010
Produced by FastQC (version 0.11.2)