FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 30h_63.3410000000594c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 30h_63.3410000000594c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1663
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTCTTAGTATCTCGTATGCCGTC1659.921828021647624No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGTC533.1870114251352977No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGTC382.285027059530968No Hit
CTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGTC342.0444978953698136No Hit
CTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGTC311.8641010222489478No Hit
CTTATACACATCTCCGAGCCCACGAGACGATCGTGTATCTCGTATGCCGTC171.0222489476849068No Hit
CTTATACACATCTCCGAGCCCACGAGACAGTGTCTTATCTCGTATGCCGTC140.841852074564041No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGTC110.661455201443175No Hit
CTTATACACATCTCCGAGCCCACGAGACGAATTGCTATCTCGTATGCCGTC70.4209260372820205RNA PCR Primer, Index 27 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGTC60.3607937462417318TruSeq Adapter, Index 12 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGT60.3607937462417318No Hit
CTTATACACATCTCCGAGCCCACGAGACTCAACTGTATCTCGTATGCCGTC50.30066145520144316No Hit
TTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGTCT40.24052916416115455Illumina PCR Primer Index 8 (95% over 21bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40.24052916416115455No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA30.1803968731208659No Hit
TTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGTCT30.1803968731208659No Hit
CTTATACAATCTCCGAGCCCACGAGACGTCTTAGTATCTCGTATGCCGTCT20.12026458208057728No Hit
TTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGTCT20.12026458208057728RNA PCR Primer, Index 27 (95% over 21bp)
ATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGTCTTC20.12026458208057728Illumina PCR Primer Index 8 (95% over 23bp)
TCCCAATGGTGTAGAAGCTATTAATGGTTCGTTTGTTCAACGATTAAAGTC20.12026458208057728No Hit
CTTATACACATCTCCGAGCCCACGAGACCTTGCAGAATCTCGTATGCCGTC20.12026458208057728No Hit
TCTTATACACATCTCCGAGCCCACGAGACCTGATTAAATCTCGTATGCCGT20.12026458208057728No Hit
TTATACACATCTCCGAGCCCACGAGACGTCTTAGTATCTCGTATGCCGTCT20.12026458208057728No Hit
CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20.12026458208057728No Hit
GCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAGTACTCTGCGTTGATAC20.12026458208057728No Hit
ATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAA20.12026458208057728No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTCCG353.2510707E-638.57142610
TGCCGTC353.2510707E-638.57142645
TATCTCG353.2510707E-638.57142636
CATCTCC307.189488E-537.4999969
ACATCTC307.189488E-537.4999968
AGCCCAC408.152649E-633.75000417
GTATGCC408.152649E-633.75000442
ATCTCGT408.152649E-633.75000437
GCCCACG408.152649E-633.75000418
CCACGAG408.152649E-633.75000420
TATGCCG408.152649E-633.75000443
GAGCCCA408.152649E-633.75000416
CACGAGA408.152649E-633.75000421
TCTCGTA408.152649E-633.75000438
ACGAGAC408.152649E-633.75000422
ATGCCGT408.152649E-633.75000444
CGTATGC408.152649E-633.75000441
CGAGCCC408.152649E-633.75000415
CCGAGCC408.152649E-633.75000414
TCGTATG408.152649E-633.75000440
Produced by FastQC (version 0.11.2)