FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 30h_62.3410000000593f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 30h_62.3410000000593f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences409481
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATTAGA250.002105724535.9993132
TCGTATG957.385097E-1026.05213740
CCCTTAG450.001225341924.9995251
CTCGTAT1001.3387762E-924.74952939
GACGGAC551.3662787E-424.5449897
TATGCCG1052.3555913E-923.57098243
GCGGTTC1353.6379788E-1223.3328911
GACCGCG1450.023.275427
CGCGGTT1405.456968E-1222.49957310
CGTTCGA500.002262389522.4995741
GCGCAAG602.464065E-422.499571
GGTTCTA1551.8189894E-1221.7737813
ATCTCGT1257.4760464E-1021.5995937
AATCTCG855.019985E-621.1760736
TTCTATT1501.4551915E-1120.99960115
GAACTGA1501.4551915E-1120.99960135
CGGTTCT1501.4551915E-1120.99960112
GCCCACG1301.2205419E-920.76883518
ATAGGAC1651.8189894E-1220.4541573
GGACCGC1651.8189894E-1220.4541576
Produced by FastQC (version 0.11.2)