##FastQC	0.11.2
>>Basic Statistics	pass
#Measure	Value
Filename	H23NVBCXX l02n01 30h_61.34100000005922.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1565
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	34.12076677316294	38.0	32.0	38.0	27.0	38.0
2	34.79297124600639	38.0	32.0	38.0	32.0	38.0
3	34.64536741214057	38.0	32.0	38.0	32.0	38.0
4	35.02811501597444	38.0	32.0	38.0	32.0	38.0
5	34.941214057507985	38.0	32.0	38.0	32.0	38.0
6	36.849840255591054	38.0	38.0	40.0	32.0	40.0
7	36.21277955271565	38.0	38.0	40.0	32.0	40.0
8	36.12460063897763	38.0	38.0	40.0	32.0	40.0
9	36.43003194888179	38.0	38.0	40.0	32.0	40.0
10	36.01022364217253	38.0	38.0	40.0	27.0	40.0
11	36.3629392971246	38.0	38.0	40.0	32.0	40.0
12	35.807667731629394	38.0	38.0	40.0	27.0	40.0
13	36.2	38.0	38.0	40.0	32.0	40.0
14	35.87092651757189	38.0	38.0	40.0	27.0	40.0
15	35.97444089456869	38.0	38.0	40.0	27.0	40.0
16	35.64984025559105	38.0	32.0	40.0	27.0	40.0
17	35.51565495207668	38.0	32.0	40.0	27.0	40.0
18	35.57444089456869	38.0	32.0	40.0	27.0	40.0
19	35.673482428115015	38.0	32.0	40.0	27.0	40.0
20	35.541214057507986	38.0	32.0	40.0	27.0	40.0
21	35.6038338658147	38.0	32.0	40.0	27.0	40.0
22	35.91054313099041	38.0	38.0	40.0	27.0	40.0
23	35.93035143769968	38.0	38.0	40.0	27.0	40.0
24	35.61661341853035	38.0	32.0	40.0	27.0	40.0
25	35.329073482428115	38.0	32.0	40.0	27.0	40.0
26	35.550159744408944	38.0	32.0	40.0	27.0	40.0
27	35.333546325878594	38.0	38.0	40.0	27.0	40.0
28	35.47284345047923	38.0	32.0	40.0	27.0	40.0
29	35.575079872204476	38.0	32.0	40.0	27.0	40.0
30	35.455591054313096	38.0	38.0	40.0	27.0	40.0
31	35.65431309904153	38.0	38.0	40.0	27.0	40.0
32	35.237699680511184	38.0	32.0	40.0	27.0	40.0
33	35.46709265175719	38.0	32.0	40.0	27.0	40.0
34	35.334185303514374	38.0	32.0	40.0	27.0	40.0
35	35.106709265175716	38.0	32.0	40.0	27.0	40.0
36	35.321405750798725	38.0	32.0	40.0	27.0	40.0
37	35.106709265175716	38.0	32.0	40.0	27.0	40.0
38	35.55782747603834	38.0	38.0	40.0	27.0	40.0
39	34.98849840255591	38.0	32.0	40.0	27.0	40.0
40	35.290095846645364	38.0	32.0	40.0	27.0	40.0
41	35.23450479233227	38.0	32.0	40.0	27.0	40.0
42	35.24920127795527	38.0	32.0	40.0	27.0	40.0
43	35.585303514376996	38.0	38.0	40.0	27.0	40.0
44	35.498402555910545	38.0	38.0	40.0	27.0	40.0
45	35.455591054313096	38.0	38.0	40.0	27.0	40.0
46	35.13162939297125	38.0	32.0	40.0	27.0	40.0
47	35.153993610223644	38.0	32.0	40.0	27.0	40.0
48	35.196805111821085	38.0	32.0	40.0	27.0	40.0
49	35.198083067092654	38.0	32.0	40.0	27.0	40.0
50	34.849201277955274	38.0	32.0	40.0	27.0	40.0
51	33.9814696485623	38.0	32.0	38.0	13.0	40.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	3.617724867724867
1101	2	3.198412698412696
1101	3	0.5264550264550323
1101	4	3.1111111111111143
1101	5	3.1891534391534435
1101	6	2.6058201058201007
1101	7	2.5899470899470813
1101	8	2.654761904761898
1101	9	2.503968253968253
1101	10	2.43386243386243
1101	11	4.191798941798943
1101	12	3.177248677248677
1101	13	2.927248677248677
1101	14	3.675925925925931
1101	15	3.283068783068778
1101	16	2.8253968253968296
1101	17	3.257936507936506
1101	18	3.9603174603174622
1101	19	3.3214285714285765
1101	20	3.257936507936506
1101	21	3.3769841269841194
1101	22	3.592592592592588
1101	23	2.828042328042322
1101	24	3.9351851851851833
1101	25	2.4761904761904745
1101	26	3.7923280423280374
1101	27	4.776455026455032
1101	28	3.624338624338627
1101	29	4.533068783068785
1101	30	4.859788359788361
1101	31	2.124338624338627
1101	32	3.5052910052910136
1101	33	3.4219576719576636
1101	34	3.727513227513228
1101	35	2.886243386243386
1101	36	5.026455026455025
1101	37	3.526455026455025
1101	38	3.739417989417994
1101	39	4.230158730158728
1101	40	4.445767195767196
1101	41	-1.5608465608465636
1101	42	3.5026455026454997
1101	43	3.9788359788359813
1101	44	3.165343915343911
1101	45	3.247354497354493
1101	46	4.11507936507936
1101	47	4.4431216931216895
1101	48	3.7804232804232853
1101	49	4.013227513227513
1101	50	0.38756613756613945
1101	51	5.740740740740748
2201	1	3.617724867724867
2201	2	0.19841269841269593
2201	3	3.5264550264550323
2201	4	3.1111111111111143
2201	5	3.1891534391534435
2201	6	2.6058201058201007
2201	7	2.5899470899470813
2201	8	1.654761904761898
2201	9	1.503968253968253
2201	10	2.43386243386243
2201	11	2.191798941798943
2201	12	2.177248677248677
2201	13	1.9272486772486772
2201	14	2.675925925925931
2201	15	3.283068783068778
2201	16	2.8253968253968296
2201	17	4.257936507936506
2201	18	3.9603174603174622
2201	19	4.3214285714285765
2201	20	4.257936507936506
2201	21	3.3769841269841194
2201	22	4.592592592592588
2201	23	3.828042328042322
2201	24	3.9351851851851833
2201	25	3.4761904761904745
2201	26	3.7923280423280374
2201	27	3.7764550264550323
2201	28	2.624338624338627
2201	29	5.533068783068785
2201	30	4.859788359788361
2201	31	4.124338624338627
2201	32	4.505291005291014
2201	33	4.421957671957664
2201	34	3.727513227513228
2201	35	4.886243386243386
2201	36	5.026455026455025
2201	37	3.526455026455025
2201	38	4.739417989417994
2201	39	4.230158730158728
2201	40	4.445767195767196
2201	41	3.9391534391534364
2201	42	3.5026455026454997
2201	43	2.9788359788359813
2201	44	4.165343915343911
2201	45	4.247354497354493
2201	46	4.11507936507936
2201	47	4.4431216931216895
2201	48	3.7804232804232853
2201	49	3.0132275132275126
2201	50	3.3875661375661394
2201	51	2.7407407407407476
2202	1	1.617724867724867
2202	2	1.198412698412696
2202	3	1.5264550264550323
2202	4	3.1111111111111143
2202	5	1.1891534391534435
2202	6	2.9391534391534364
2202	7	2.923280423280417
2202	8	2.9880952380952337
2202	9	2.1706349206349174
2202	10	2.1005291005290943
2202	11	2.191798941798943
2202	12	-1.489417989417987
2202	13	3.260582010582013
2202	14	2.675925925925931
2202	15	1.6164021164021136
2202	16	2.492063492063494
2202	17	2.9246031746031704
2202	18	3.293650793650798
2202	19	0.9880952380952408
2202	20	2.257936507936506
2202	21	3.710317460317455
2202	22	3.925925925925924
2202	23	-4.838624338624346
2202	24	-4.7314814814814845
2202	25	3.142857142857139
2202	26	2.7923280423280374
2202	27	3.4431216931216966
2202	28	3.291005291005291
2202	29	4.866402116402121
2202	30	4.859788359788361
2202	31	2.791005291005291
2202	32	3.8386243386243493
2202	33	-4.578042328042336
2202	34	2.394179894179892
2202	35	2.2195767195767218
2202	36	5.359788359788361
2202	37	3.859788359788361
2202	38	4.07275132275133
2202	39	4.563492063492063
2202	40	4.1124338624338606
2202	41	1.6058201058201007
2202	42	1.169312169312164
2202	43	1.6455026455026456
2202	44	3.832010582010575
2202	45	1.9140211640211575
2202	46	-4.551587301587308
2202	47	1.1097883597883538
2202	48	5.113756613756621
2202	49	4.346560846560848
2202	50	2.720899470899475
2202	51	5.407407407407412
1102	1	-6.882275132275133
1102	2	0.44841269841269593
1102	3	-0.7235449735449677
1102	4	-3.1388888888888857
1102	5	-1.0608465608465565
1102	6	-0.6441798941798993
1102	7	-6.410052910052919
1102	8	-6.345238095238102
1102	9	-0.24603174603174693
1102	10	-3.06613756613757
1102	11	0.1917989417989432
1102	12	2.677248677248677
1102	13	-0.3227513227513228
1102	14	-2.574074074074069
1102	15	1.783068783068778
1102	16	0.07539682539682957
1102	17	0.5079365079365061
1102	18	-0.28968253968253777
1102	19	-2.4285714285714235
1102	20	0.5079365079365061
1102	21	3.8769841269841194
1102	22	3.592592592592588
1102	23	1.0780423280423221
1102	24	-2.3148148148148167
1102	25	0.2261904761904745
1102	26	1.0423280423280374
1102	27	3.2764550264550323
1102	28	1.6243386243386269
1102	29	3.533068783068785
1102	30	-2.890211640211639
1102	31	3.124338624338627
1102	32	3.0052910052910136
1102	33	3.4219576719576636
1102	34	3.227513227513228
1102	35	3.386243386243386
1102	36	-1.7235449735449748
1102	37	-3.223544973544975
1102	38	-2.510582010582006
1102	39	4.230158730158728
1102	40	1.1957671957671963
1102	41	2.4391534391534364
1102	42	3.5026455026454997
1102	43	-1.7711640211640187
1102	44	3.665343915343911
1102	45	2.747354497354493
1102	46	-2.1349206349206398
1102	47	3.9431216931216895
1102	48	1.5304232804232853
1102	49	2.0132275132275126
1102	50	3.8875661375661394
1102	51	1.7407407407407476
2203	1	3.617724867724867
2203	2	3.198412698412696
2203	3	1.5264550264550323
2203	4	-0.8888888888888857
2203	5	1.1891534391534435
2203	6	0.272486772486765
2203	7	1.5899470899470813
2203	8	1.654761904761898
2203	9	-0.49603174603174693
2203	10	1.43386243386243
2203	11	0.1917989417989432
2203	12	2.177248677248677
2203	13	1.9272486772486772
2203	14	3.3425925925925952
2203	15	-5.38359788359789
2203	16	0.49206349206349387
2203	17	-1.7420634920634939
2203	18	-3.0396825396825378
2203	19	0.9880952380952408
2203	20	0.25793650793650613
2203	21	-0.9563492063492163
2203	22	-7.740740740740744
2203	23	-3.8386243386243457
2203	24	-4.7314814814814845
2203	25	-1.1904761904761898
2203	26	2.7923280423280374
2203	27	1.4431216931216966
2203	28	-7.708994708994705
2203	29	-0.13359788359787927
2203	30	0.8597883597883609
2203	31	-1.8756613756613731
2203	32	0.5052910052910136
2203	33	0.42195767195766365
2203	34	0.3941798941798922
2203	35	2.886243386243386
2203	36	4.026455026455025
2203	37	-1.4735449735449748
2203	38	-2.92724867724867
2203	39	-4.43650793650794
2203	40	-4.220899470899472
2203	41	-4.727513227513231
2203	42	-0.4973544973545003
2203	43	-0.02116402116401872
2203	44	3.165343915343911
2203	45	-4.419312169312175
2203	46	1.7817460317460245
2203	47	3.4431216931216895
2203	48	-6.552910052910047
2203	49	1.0132275132275126
2203	50	1.3875661375661394
2203	51	-3.5925925925925846
1103	1	-1.882275132275133
1103	2	-2.801587301587304
1103	3	-8.973544973544968
1103	4	-5.388888888888886
1103	5	-5.3108465608465565
1103	6	1.6058201058201007
1103	7	-1.4100529100529187
1103	8	-10.845238095238102
1103	9	-1.496031746031747
1103	10	1.43386243386243
1103	11	-3.308201058201057
1103	12	2.177248677248677
1103	13	-9.572751322751323
1103	14	-1.824074074074069
1103	15	-10.216931216931222
1103	16	-3.6746031746031704
1103	17	2.257936507936506
1103	18	-13.539682539682538
1103	19	2.3214285714285765
1103	20	2.257936507936506
1103	21	2.3769841269841194
1103	22	2.592592592592588
1103	23	2.828042328042322
1103	24	-0.06481481481481666
1103	25	2.4761904761904745
1103	26	-0.20767195767196256
1103	27	2.7764550264550323
1103	28	2.624338624338627
1103	29	-8.966931216931215
1103	30	0.8597883597883609
1103	31	0.12433862433862686
1103	32	2.5052910052910136
1103	33	0.42195767195766365
1103	34	-1.272486772486772
1103	35	-2.613756613756614
1103	36	4.026455026455025
1103	37	-0.4735449735449748
1103	38	-9.760582010582006
1103	39	-2.2698412698412724
1103	40	-9.054232804232804
1103	41	2.9391534391534364
1103	42	-9.9973544973545
1103	43	-9.521164021164019
1103	44	-2.334656084656089
1103	45	-8.252645502645507
1103	46	4.11507936507936
1103	47	-1.0568783068783105
1103	48	-8.719576719576715
1103	49	4.013227513227513
1103	50	-8.11243386243386
1103	51	-0.7592592592592524
2204	1	0.617724867724867
2204	2	3.198412698412696
2204	3	2.0264550264550323
2204	4	1.6111111111111143
2204	5	0.18915343915344351
2204	6	1.1058201058201007
2204	7	2.0899470899470813
2204	8	2.154761904761898
2204	9	2.003968253968253
2204	10	-3.81613756613757
2204	11	-0.05820105820105681
2204	12	2.677248677248677
2204	13	2.427248677248677
2204	14	3.675925925925931
2204	15	1.783068783068778
2204	16	2.3253968253968296
2204	17	1.7579365079365061
2204	18	3.4603174603174622
2204	19	-1.4285714285714235
2204	20	1.7579365079365061
2204	21	1.8769841269841194
2204	22	1.5925925925925881
2204	23	-4.421957671957678
2204	24	0.43518518518518334
2204	25	-3.2738095238095255
2204	26	0.5423280423280374
2204	27	1.7764550264550323
2204	28	1.6243386243386269
2204	29	0.283068783068785
2204	30	1.8597883597883609
2204	31	2.624338624338627
2204	32	3.5052910052910136
2204	33	2.9219576719576636
2204	34	-5.522486772486772
2204	35	-0.863756613756614
2204	36	2.026455026455025
2204	37	3.026455026455025
2204	38	0.2394179894179942
2204	39	-2.5198412698412724
2204	40	-2.3042328042328037
2204	41	3.4391534391534364
2204	42	2.0026455026454997
2204	43	-2.2711640211640187
2204	44	2.165343915343911
2204	45	3.747354497354493
2204	46	2.6150793650793602
2204	47	0.4431216931216895
2204	48	3.7804232804232853
2204	49	3.5132275132275126
2204	50	4.3875661375661394
2204	51	-2.5092592592592524
2205	1	0.617724867724867
2205	2	-5.301587301587304
2205	3	3.5264550264550323
2205	4	0.11111111111111427
2205	5	-2.8108465608465565
2205	6	-2.8941798941798993
2205	7	-3.9100529100529187
2205	8	-9.845238095238102
2205	9	-2.996031746031747
2205	10	-3.06613756613757
2205	11	0.1917989417989432
2205	12	3.177248677248677
2205	13	2.927248677248677
2205	14	0.6759259259259309
2205	15	0.2830687830687779
2205	16	-0.17460317460317043
2205	17	0.25793650793650613
2205	18	-10.539682539682538
2205	19	-3.1785714285714235
2205	20	-0.7420634920634939
2205	21	-3.1230158730158806
2205	22	-2.907407407407412
2205	23	0.8280423280423221
2205	24	-0.06481481481481666
2205	25	-3.0238095238095255
2205	26	-1.7076719576719626
2205	27	3.7764550264550323
2205	28	0.6243386243386269
2205	29	1.533068783068785
2205	30	0.8597883597883609
2205	31	-0.8756613756613731
2205	32	-2.9947089947089864
2205	33	3.4219576719576636
2205	34	1.727513227513228
2205	35	-0.11375661375661394
2205	36	1.0264550264550252
2205	37	3.526455026455025
2205	38	2.739417989417994
2205	39	0.23015873015872756
2205	40	-8.054232804232804
2205	41	-1.5608465608465636
2205	42	-2.9973544973545003
2205	43	-1.5211640211640187
2205	44	-2.334656084656089
2205	45	-1.2526455026455068
2205	46	-9.38492063492064
2205	47	-1.0568783068783105
2205	48	-7.719576719576715
2205	49	4.013227513227513
2205	50	4.3875661375661394
2205	51	0.24074074074074758
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1209	9	2.503968253968253
1209	10	-11.06613756613757
1209	11	-9.308201058201057
1209	12	-2.322751322751323
1209	13	-3.572751322751323
1209	14	2.675925925925931
1209	15	0.2830687830687779
1209	16	-10.67460317460317
1209	17	-0.7420634920634939
1209	18	-2.5396825396825378
1209	19	0.3214285714285765
1209	20	-2.242063492063494
1209	21	-2.1230158730158806
1209	22	-2.907407407407412
1209	23	-1.6719576719576779
1209	24	0.9351851851851833
1209	25	-9.023809523809526
1209	26	-8.707671957671963
1209	27	-8.723544973544968
1209	28	-1.8756613756613731
1209	29	-7.966931216931215
1209	30	0.8597883597883609
1209	31	-9.375661375661373
1209	32	-1.9947089947089864
1209	33	0.42195767195766365
1209	34	-10.772486772486772
1209	35	-9.613756613756614
1209	36	-20.973544973544975
1209	37	-9.973544973544975
1209	38	-2.760582010582006
1209	39	-2.2698412698412724
1209	40	-9.054232804232804
1209	41	0.9391534391534364
1209	42	-8.9973544973545
1209	43	-2.5211640211640187
1209	44	-1.334656084656089
1209	45	-8.252645502645507
1209	46	-8.38492063492064
1209	47	-8.05687830687831
1209	48	4.780423280423285
1209	49	-2.4867724867724874
1209	50	-2.1124338624338606
1209	51	-0.7592592592592524
1214	1	0.617724867724867
1214	2	0.19841269841269593
1214	3	0.5264550264550323
1214	4	3.1111111111111143
1214	5	0.18915343915344351
1214	6	-2.8941798941798993
1214	7	-0.41005291005291866
1214	8	2.654761904761898
1214	9	2.503968253968253
1214	10	-0.56613756613757
1214	11	3.191798941798943
1214	12	3.177248677248677
1214	13	2.927248677248677
1214	14	4.675925925925931
1214	15	3.283068783068778
1214	16	2.8253968253968296
1214	17	3.257936507936506
1214	18	2.9603174603174622
1214	19	0.3214285714285765
1214	20	-2.242063492063494
1214	21	3.3769841269841194
1214	22	0.5925925925925881
1214	23	3.828042328042322
1214	24	-1.5648148148148167
1214	25	3.4761904761904745
1214	26	3.7923280423280374
1214	27	4.776455026455032
1214	28	2.624338624338627
1214	29	3.533068783068785
1214	30	2.859788359788361
1214	31	2.124338624338627
1214	32	-0.49470899470898644
1214	33	3.4219576719576636
1214	34	2.727513227513228
1214	35	-12.613756613756614
1214	36	4.026455026455025
1214	37	2.526455026455025
1214	38	0.7394179894179942
1214	39	-8.269841269841272
1214	40	-1.0542328042328037
1214	41	0.9391534391534364
1214	42	-8.9973544973545
1214	43	-1.5211640211640187
1214	44	3.165343915343911
1214	45	-9.252645502645507
1214	46	-9.38492063492064
1214	47	4.4431216931216895
1214	48	1.7804232804232853
1214	49	4.013227513227513
1214	50	1.3875661375661394
1214	51	5.740740740740748
1215	1	3.617724867724867
1215	2	3.198412698412696
1215	3	1.5264550264550323
1215	4	3.1111111111111143
1215	5	3.1891534391534435
1215	6	2.9391534391534364
1215	7	2.923280423280417
1215	8	2.9880952380952337
1215	9	3.503968253968253
1215	10	2.7671957671957657
1215	11	1.525132275132279
1215	12	2.8439153439153415
1215	13	3.260582010582013
1215	14	4.009259259259267
1215	15	2.283068783068778
1215	16	2.492063492063494
1215	17	2.257936507936506
1215	18	1.9603174603174622
1215	19	2.988095238095241
1215	20	2.9246031746031704
1215	21	3.710317460317455
1215	22	4.592592592592588
1215	23	4.161375661375658
1215	24	4.935185185185183
1215	25	4.4761904761904745
1215	26	2.125661375661373
1215	27	3.4431216931216966
1215	28	3.291005291005291
1215	29	4.199735449735449
1215	30	4.193121693121697
1215	31	2.791005291005291
1215	32	3.171957671957678
1215	33	4.421957671957664
1215	34	2.394179894179892
1215	35	-4.780423280423282
1215	36	2.6931216931216895
1215	37	3.1931216931216895
1215	38	3.4060846560846585
1215	39	3.896825396825392
1215	40	4.1124338624338606
1215	41	3.6058201058201007
1215	42	3.8359788359788354
1215	43	4.978835978835981
1215	44	4.498677248677247
1215	45	4.580687830687829
1215	46	3.7817460317460245
1215	47	4.776455026455025
1215	48	5.780423280423285
1215	49	5.013227513227513
1215	50	5.3875661375661394
1215	51	4.740740740740748
1212	1	3.617724867724867
1212	2	0.19841269841269593
1212	3	0.5264550264550323
1212	4	0.11111111111111427
1212	5	0.18915343915344351
1212	6	-0.3941798941798993
1212	7	2.5899470899470813
1212	8	2.654761904761898
1212	9	-0.49603174603174693
1212	10	-0.56613756613757
1212	11	-10.308201058201057
1212	12	2.177248677248677
1212	13	1.9272486772486772
1212	14	-0.3240740740740691
1212	15	2.283068783068778
1212	16	2.8253968253968296
1212	17	2.257936507936506
1212	18	3.9603174603174622
1212	19	4.3214285714285765
1212	20	3.257936507936506
1212	21	2.3769841269841194
1212	22	3.592592592592588
1212	23	0.8280423280423221
1212	24	0.9351851851851833
1212	25	3.4761904761904745
1212	26	3.7923280423280374
1212	27	2.7764550264550323
1212	28	2.624338624338627
1212	29	3.533068783068785
1212	30	2.859788359788361
1212	31	2.124338624338627
1212	32	2.5052910052910136
1212	33	0.42195767195766365
1212	34	-0.2724867724867721
1212	35	0.886243386243386
1212	36	1.0264550264550252
1212	37	-1.9735449735449748
1212	38	0.7394179894179942
1212	39	-2.2698412698412724
1212	40	-8.054232804232804
1212	41	2.9391534391534364
1212	42	2.5026455026454997
1212	43	-0.02116402116401872
1212	44	4.165343915343911
1212	45	4.247354497354493
1212	46	4.11507936507936
1212	47	-8.05687830687831
1212	48	-0.7195767195767147
1212	49	3.0132275132275126
1212	50	4.3875661375661394
1212	51	1.7407407407407476
1213	1	3.617724867724867
1213	2	-2.801587301587304
1213	3	-2.4735449735449677
1213	4	-2.8888888888888857
1213	5	-21.810846560846556
1213	6	-9.3941798941799
1213	7	-4.410052910052919
1213	8	1.654761904761898
1213	9	1.503968253968253
1213	10	1.43386243386243
1213	11	2.191798941798943
1213	12	2.177248677248677
1213	13	1.9272486772486772
1213	14	-22.32407407407407
1213	15	-3.716931216931222
1213	16	1.8253968253968296
1213	17	2.257936507936506
1213	18	1.9603174603174622
1213	19	2.3214285714285765
1213	20	2.257936507936506
1213	21	-3.6230158730158806
1213	22	-3.407407407407412
1213	23	-3.171957671957678
1213	24	2.9351851851851833
1213	25	-3.5238095238095255
1213	26	-3.2076719576719626
1213	27	2.7764550264550323
1213	28	-22.375661375661373
1213	29	-21.466931216931215
1213	30	-8.14021164021164
1213	31	2.124338624338627
1213	32	-3.4947089947089864
1213	33	-3.5780423280423364
1213	34	1.727513227513228
1213	35	2.886243386243386
1213	36	-1.9735449735449748
1213	37	-3.473544973544975
1213	38	-8.260582010582006
1213	39	-2.7698412698412724
1213	40	3.4457671957671963
1213	41	-22.060846560846564
1213	42	-8.4973544973545
1213	43	-22.02116402116402
1213	44	-7.834656084656089
1213	45	-7.752645502645507
1213	46	-21.88492063492064
1213	47	-21.55687830687831
1213	48	-21.219576719576715
1213	49	-21.986772486772487
1213	50	-21.61243386243386
1213	51	-20.259259259259252
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
22	2.0
23	4.0
24	9.0
25	11.0
26	19.0
27	26.0
28	41.0
29	63.0
30	50.0
31	73.0
32	80.0
33	98.0
34	110.0
35	112.0
36	143.0
37	191.0
38	280.0
39	253.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.670926517571885	14.824281150159743	10.415335463258787	44.08945686900959
2	10.670926517571885	17.82747603833866	50.47923322683706	21.022364217252395
3	13.6741214057508	20.319488817891372	43.70607028753994	22.30031948881789
4	10.798722044728434	31.69329073482428	31.629392971246006	25.878594249201274
5	11.054313099041533	29.712460063897762	46.64536741214057	12.587859424920127
6	23.833865814696487	48.115015974440894	15.527156549520768	12.523961661341854
7	21.98083067092652	26.325878594249204	18.594249201277954	33.09904153354633
8	18.402555910543132	50.926517571884986	16.102236421725237	14.568690095846645
9	20.702875399361023	14.440894568690096	17.444089456869012	47.41214057507987
10	13.354632587859424	40.319488817891376	27.15654952076677	19.169329073482427
11	24.34504792332268	19.7444089456869	38.849840255591054	17.06070287539936
12	16.86900958466454	19.808306709265175	27.539936102236425	35.78274760383386
13	20.95846645367412	16.166134185303516	41.405750798722046	21.46964856230032
14	15.910543130990416	17.444089456869012	22.42811501597444	44.21725239616613
15	21.08626198083067	22.236421725239616	19.297124600638977	37.38019169329074
16	35.01597444089457	22.81150159744409	22.55591054313099	19.616613418530353
17	19.233226837060702	40.83067092651757	20.383386581469647	19.552715654952078
18	36.76470588235294	23.721227621483376	21.483375959079286	18.0306905370844
19	19.424920127795527	20.95846645367412	22.236421725239616	37.38019169329074
20	18.466453674121404	21.21405750798722	21.661341853035143	38.65814696485623
21	16.357827476038338	23.961661341853034	20.12779552715655	39.552715654952074
22	17.955271565495206	39.04153354632588	21.72523961661342	21.277955271565496
23	19.808306709265175	21.46964856230032	22.93929712460064	35.78274760383386
24	35.71884984025559	23.130990415335464	22.55591054313099	18.594249201277954
25	20.447284345047922	38.65814696485623	21.405750798722046	19.488817891373802
26	36.16613418530352	23.067092651757186	22.683706070287542	18.08306709265176
27	19.616613418530353	40.2555910543131	21.597444089456868	18.53035143769968
28	20.383386581469647	22.81150159744409	21.022364217252395	35.78274760383386
29	24.98402555910543	25.495207667731627	31.24600638977636	18.27476038338658
30	18.849840255591054	30.47923322683706	29.26517571884984	21.405750798722046
31	24.15335463258786	25.111821086261983	27.47603833865815	23.258785942492015
32	23.258785942492015	27.3482428115016	26.00638977635783	23.38658146964856
33	19.936102236421725	25.878594249201274	32.97124600638978	21.21405750798722
34	26.5814696485623	21.916932907348244	27.092651757188495	24.40894568690096
35	23.706070287539934	27.284345047923324	24.472843450479235	24.536741214057507
36	17.316293929712458	27.539936102236425	33.9297124600639	21.21405750798722
37	17.57188498402556	36.99680511182108	24.728434504792332	20.702875399361023
38	16.421725239616613	18.210862619808307	44.66453674121406	20.702875399361023
39	17.252396166134183	18.594249201277954	27.79552715654952	36.35782747603834
40	17.316293929712458	17.89137380191693	42.30031948881789	22.492012779552716
41	17.57188498402556	17.57188498402556	27.47603833865815	37.38019169329074
42	33.418530351437695	17.18849840255591	28.817891373801917	20.575079872204473
43	14.88817891373802	18.91373801916933	44.34504792332268	21.85303514376997
44	13.418530351437699	36.29392971246006	30.095846645367413	20.191693290734825
45	14.504792332268371	19.297124600638977	45.62300319488818	20.575079872204473
46	30.415335463258785	19.680511182108624	28.306709265175716	21.597444089456868
47	13.482428115015974	20.447284345047922	26.517571884984026	39.552715654952074
48	13.354632587859424	19.7444089456869	26.389776357827476	40.5111821086262
49	28.753993610223645	19.936102236421725	27.987220447284344	23.322683706070286
50	13.546325878594248	21.08626198083067	41.66134185303515	23.706070287539934
51	11.437699680511182	22.364217252396166	24.600638977635782	41.59744408945687
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.5
2	1.0
3	1.0
4	1.0
5	1.0
6	1.0
7	0.5
8	0.0
9	0.0
10	0.0
11	0.5
12	1.0
13	1.0
14	1.0
15	1.0
16	1.0
17	1.5
18	2.0
19	1.0
20	0.0
21	0.5
22	1.0
23	1.5
24	2.0
25	1.5
26	1.5
27	2.0
28	6.0
29	10.0
30	11.5
31	13.0
32	16.5
33	20.0
34	35.5
35	51.0
36	67.5
37	84.0
38	96.5
39	109.0
40	118.0
41	127.0
42	124.5
43	122.0
44	131.0
45	140.0
46	129.5
47	119.0
48	144.0
49	169.0
50	194.0
51	219.0
52	213.0
53	207.0
54	135.5
55	64.0
56	50.0
57	36.0
58	28.0
59	20.0
60	13.5
61	7.0
62	10.5
63	14.0
64	11.0
65	8.0
66	5.5
67	3.0
68	3.5
69	4.0
70	3.0
71	2.0
72	2.0
73	2.0
74	1.5
75	0.5
76	0.0
77	0.0
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.06389776357827476
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1565.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	82.74760383386581
#Duplication Level	Percentage of deduplicated	Percentage of total
1	98.06949806949807	81.15015974440894
2	0.5405405405405406	0.8945686900958467
3	0.15444015444015444	0.38338658146964855
4	0.4633204633204633	1.5335463258785942
5	0.15444015444015444	0.6389776357827476
6	0.0	0.0
7	0.07722007722007722	0.44728434504792336
8	0.0	0.0
9	0.0	0.0
>10	0.4633204633204633	10.60702875399361
>50	0.07722007722007722	4.345047923322683
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGTC	68	4.345047923322683	No Hit
CTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGTC	44	2.8115015974440896	No Hit
CTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGTC	43	2.747603833865815	No Hit
CTTATACACATCTCCGAGCCCACGAGACATAGACAAATCTCGTATGCCGTC	31	1.9808306709265175	No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGTC	24	1.5335463258785942	No Hit
CTTATACACATCTCCGAGCCCACGAGACGAATTGCTATCTCGTATGCCGTC	12	0.7667731629392971	RNA PCR Primer, Index 27 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACGTCTTAGTATCTCGTATGCCGTC	12	0.7667731629392971	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATAGACAAATCTCGTATGCCG	7	0.44728434504792336	No Hit
CTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGTC	5	0.3194888178913738	No Hit
CTTATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGTC	5	0.3194888178913738	TruSeq Adapter, Index 12 (95% over 21bp)
TTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGTCT	4	0.25559105431309903	Illumina PCR Primer Index 8 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACATAGACAAATCTCGTATGCCGT	4	0.25559105431309903	No Hit
TCTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGT	4	0.25559105431309903	No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGTC	4	0.25559105431309903	No Hit
TTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGTCT	4	0.25559105431309903	No Hit
CTTATACACATCTCCGAGCCCACGAGACAATGCGTTATCTCGTATGCCGTC	4	0.25559105431309903	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCG	3	0.19169329073482427	No Hit
CTTATACACATCTCCGAGCCCACGAGACCTGATTAAATCTCGTATGCCGTC	3	0.19169329073482427	No Hit
CTTATACACATCTCCGAGCCCACGAGACTGATTACAATCTCGTATGCCGTC	2	0.12779552715654952	No Hit
CCCCCACACGCGACAACCGCCCCAGCCAACGTAGAAAAGCCAGAGCCGCGG	2	0.12779552715654952	No Hit
TCTTATACACATCTCCGAGCCCACGAGACGTCTTAGTATCTCGTATGCCGT	2	0.12779552715654952	No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCTACTTATCTCGTATGCCGTC	2	0.12779552715654952	No Hit
TCTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGT	2	0.12779552715654952	No Hit
CCTCATCATTAACTGTGGCAACTTGGTTTATCAGTCCTGCGGAACCACTGC	2	0.12779552715654952	No Hit
TATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGTCTT	2	0.12779552715654952	Illumina PCR Primer Index 4 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA Adapter	Nextera Transposase Sequence
1	0.0	0.0	0.0
2	0.0	0.0	0.0
3	0.0	0.0	0.0
4	0.0	0.0	0.0
5	0.0	0.0	0.0
6	0.0	0.0	0.0
7	0.0	0.0	0.0
8	0.0	0.0	0.0
9	0.0	0.0	0.0
10	0.0	0.0	0.0
11	0.0	0.0	0.0
12	0.0	0.0	0.0
13	0.0	0.0	0.0
14	0.0	0.0	0.0
15	0.0	0.0	0.0
16	0.0	0.0	0.0
17	0.0	0.0	0.0
18	0.0	0.0	0.0
19	0.0	0.0	0.0
20	0.0	0.0	0.0
21	0.0	0.0	0.0
22	0.0	0.0	0.06389776357827476
23	0.0	0.0	0.06389776357827476
24	0.0	0.0	0.25559105431309903
25	0.0	0.0	0.25559105431309903
26	0.0	0.0	0.3194888178913738
27	0.0	0.0	0.3194888178913738
28	0.0	0.0	0.4472843450479233
29	0.0	0.0	0.7667731629392971
30	0.0	0.0	1.2779552715654952
31	0.0	0.0	2.5559105431309903
32	0.0	0.0	3.7060702875399363
33	0.0	0.0	4.984025559105431
34	0.0	0.0	6.198083067092652
35	0.0	0.0	7.220447284345048
36	0.0	0.0	9.00958466453674
37	0.0	0.0	11.309904153354633
38	0.0	0.0	13.993610223642172
39	0.0	0.0	16.230031948881788
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCTATCT	20	5.0708407E-4	45.000004	34
CGATAAC	20	5.0708407E-4	45.000004	28
GATAACC	20	5.0708407E-4	45.000004	29
AACCTAT	20	5.0708407E-4	45.000004	32
ATAACCT	20	5.0708407E-4	45.000004	30
TAACCTA	20	5.0708407E-4	45.000004	31
AGACGAT	20	5.0708407E-4	45.000004	25
GACGATA	20	5.0708407E-4	45.000004	26
ACCTATC	20	5.0708407E-4	45.000004	33
ACGATAA	20	5.0708407E-4	45.000004	27
CTATCTC	20	5.0708407E-4	45.000004	35
TATCTCG	25	2.36841E-5	45.0	36
CGAGACG	25	2.36841E-5	45.0	23
GAGACGA	25	2.36841E-5	45.0	24
TGCCGTC	30	1.0716958E-6	44.999996	45
ATGCCGT	30	1.0716958E-6	44.999996	44
AGCCCAC	35	3.1055934E-6	38.571426	17
GTATGCC	35	3.1055934E-6	38.571426	42
ATCTCGT	35	3.1055934E-6	38.571426	37
ATCTCCG	35	3.1055934E-6	38.571426	10
>>END_MODULE