H23NVBCXX l02n01 30h_61.34100000005922.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	H23NVBCXX l02n01 30h_61.34100000005922.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1565
Sequences flagged as poor quality	0
Sequence length	51
%GC	46

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGTC	68	4.345047923322683	No Hit
CTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGTC	44	2.8115015974440896	No Hit
CTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGTC	43	2.747603833865815	No Hit
CTTATACACATCTCCGAGCCCACGAGACATAGACAAATCTCGTATGCCGTC	31	1.9808306709265175	No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGTC	24	1.5335463258785942	No Hit
CTTATACACATCTCCGAGCCCACGAGACGAATTGCTATCTCGTATGCCGTC	12	0.7667731629392971	RNA PCR Primer, Index 27 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACGTCTTAGTATCTCGTATGCCGTC	12	0.7667731629392971	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATAGACAAATCTCGTATGCCG	7	0.44728434504792336	No Hit
CTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGTC	5	0.3194888178913738	No Hit
CTTATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGTC	5	0.3194888178913738	TruSeq Adapter, Index 12 (95% over 21bp)
TTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGTCT	4	0.25559105431309903	Illumina PCR Primer Index 8 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACATAGACAAATCTCGTATGCCGT	4	0.25559105431309903	No Hit
TCTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGT	4	0.25559105431309903	No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGTC	4	0.25559105431309903	No Hit
TTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGTCT	4	0.25559105431309903	No Hit
CTTATACACATCTCCGAGCCCACGAGACAATGCGTTATCTCGTATGCCGTC	4	0.25559105431309903	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCG	3	0.19169329073482427	No Hit
CTTATACACATCTCCGAGCCCACGAGACCTGATTAAATCTCGTATGCCGTC	3	0.19169329073482427	No Hit
CTTATACACATCTCCGAGCCCACGAGACTGATTACAATCTCGTATGCCGTC	2	0.12779552715654952	No Hit
CCCCCACACGCGACAACCGCCCCAGCCAACGTAGAAAAGCCAGAGCCGCGG	2	0.12779552715654952	No Hit
TCTTATACACATCTCCGAGCCCACGAGACGTCTTAGTATCTCGTATGCCGT	2	0.12779552715654952	No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCTACTTATCTCGTATGCCGTC	2	0.12779552715654952	No Hit
TCTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGT	2	0.12779552715654952	No Hit
CCTCATCATTAACTGTGGCAACTTGGTTTATCAGTCCTGCGGAACCACTGC	2	0.12779552715654952	No Hit
TATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGTCTT	2	0.12779552715654952	Illumina PCR Primer Index 4 (95% over 21bp)

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCTATCT	20	5.0708407E-4	45.000004	34
CGATAAC	20	5.0708407E-4	45.000004	28
GATAACC	20	5.0708407E-4	45.000004	29
AACCTAT	20	5.0708407E-4	45.000004	32
ATAACCT	20	5.0708407E-4	45.000004	30
TAACCTA	20	5.0708407E-4	45.000004	31
AGACGAT	20	5.0708407E-4	45.000004	25
GACGATA	20	5.0708407E-4	45.000004	26
ACCTATC	20	5.0708407E-4	45.000004	33
ACGATAA	20	5.0708407E-4	45.000004	27
CTATCTC	20	5.0708407E-4	45.000004	35
TATCTCG	25	2.36841E-5	45.0	36
CGAGACG	25	2.36841E-5	45.0	23
GAGACGA	25	2.36841E-5	45.0	24
TGCCGTC	30	1.0716958E-6	44.999996	45
ATGCCGT	30	1.0716958E-6	44.999996	44
AGCCCAC	35	3.1055934E-6	38.571426	17
GTATGCC	35	3.1055934E-6	38.571426	42
ATCTCGT	35	3.1055934E-6	38.571426	37
ATCTCCG	35	3.1055934E-6	38.571426	10

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content