FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 30h_60.34100000005915.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 30h_60.34100000005915.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences522282
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCACTA250.002106344635.99900416
CTATACC551.1259726E-732.7263684
GGTATCA3400.031.1169741
GTATCAA8000.026.4494291
TTAGATA500.002263332322.4993764
GGCCTAG550.00392343920.4637761
GTATACT550.00393433720.453984
ATCTCCG6450.019.88507545
GTATGGG807.526763E-519.6963831
CATCTCC17250.019.30566245
ACGGGGC2100.019.28518116
CTAGGCA706.977787E-419.2851819
TATCAAC11050.018.9361292
CGGGGCT1253.8244252E-717.99950217
GGGGGGG2001.8189894E-1217.99950218
GTAGGGG1153.4422865E-617.6082083
TCAACGC12000.017.4370174
AACGCAG12050.017.3646666
CAACGCA12250.017.0811615
ACGCAGA12400.016.8745357
Produced by FastQC (version 0.11.2)