FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 30h_6.34100000005583.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 30h_6.34100000005583.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences532934
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAATAATAGGATTCCCAATCGTTGTAGCCATCATTATATTTCCTTCAATCC19890.37321694618845863No Hit
GAATAGGATTGAAGGAAATATAATGATGGCTACAACGATTGGGAATCCTAT10010.1878281363170674No Hit
TTCTAGGAGTCTGCCTAATAGTCCAAATCATTACAGGTCTTTTCTTAGCCA9730.18257420243407252No Hit
CATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCATTCCAC9670.18144835945914503No Hit
GTTGTTGATTAGGCGTTTTGAGGATGGGAATAGGATTGAAGGAAATATAAT8100.1519888016152094No Hit
CTACACATCAGATACAATAACAGCCTTTTCATCAGTAACACACATTTGTCG7450.1397921693868284No Hit
TCATTATATTTCCTTCAATCCTATTCCCATCCTCAAAACGCCTAATCAACA7180.13472587599965474No Hit
GTATATATCGGATTAGTCACCCGTAATTTACGTCTCGACAAATGTGTGTTA6610.1240303677378437No Hit
ATTCTAGAATTTGCAGTAGCATTAATTCAAGCCTACGTATTCACCCTCCTA6050.11352249997185393No Hit
CTATTATTGTTGGGGTAATGAATGAGGCAAATAGATTTTCGTTCATTTTAA5810.10901912807214402No Hit
CCATAAAAGTAAAAACCCCTTGAGAATTAAAATGAACGAAAATCTATTTGC5700.10695508261811032No Hit
GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACTG5470.10263935121422164No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAACGA300.00514564929.9989995
TAAGCCG901.2732926E-1129.99899932
CCGGACT952.3646862E-1128.42010336
TATGCCG406.1629695E-428.12406343
GTATCAA5150.027.9654791
TATCTCG507.152942E-526.99909836
GATCGTA802.9658113E-622.50347312
ATATGTA1800.022.5034711
GCTAATG1204.5110937E-1022.4992543
AAGCGTG702.7001763E-522.49924923
AACTATC5200.022.49924919
CCACGAG500.00226346722.49924920
ACTACCC5150.022.2849912
ACTATCC5250.022.28497120
GGTATCA1750.021.8605161
CGGACTG1257.4942363E-1021.59928137
TCCGATT2200.021.48058716
CTATCCA5500.021.27201821
TGTCCGC754.597866E-520.999310
CCAACTA5700.020.9242817
Produced by FastQC (version 0.11.2)