FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 30h_59.34100000005908.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 30h_59.34100000005908.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences488650
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCCG301.1385347E-437.5023885
GGTAGCG250.00210526836.0022937
TATGCCG606.4192136E-933.74869543
ACGCCTA352.818659E-432.14161721
TAGGCGT300.00514536929.99884231
CGTAAAC507.1520044E-526.99895532
TCGTATG957.403287E-1026.05162440
GGTATCA3400.025.8131081
GTATCAA6400.025.3167041
CGCCTAA450.00122582224.99903322
CCGACCC450.00122582224.9990334
CCCGACC450.00122582224.9990333
ACTTTAG702.6954263E-522.5037361
GTAATAG500.002260579322.5037351
GACCCGG702.6975587E-522.5014326
GCTCGCA500.002261924322.50143215
CGCAATG602.465339E-422.49913218
CGTATGC1003.6350684E-822.4991341
CCACGAG1156.7484507E-921.52090620
CGAGCCC1056.085611E-821.42993515
Produced by FastQC (version 0.11.2)