FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 30h_58.341000000058fa.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 30h_58.341000000058fa.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences462460
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA2950.032.0570031
GTATCAA7500.027.6199071
CGTCCCC507.1510614E-526.99902714
GCCCACG608.180006E-626.24905418
TATGCCG608.180006E-626.24905443
TATAGTA608.180006E-626.2490542
GCTTTAA500.002253116622.516231
TAATCGC602.463214E-422.50162325
CGCCTAA903.2758726E-722.50162122
TAGGGGT500.00226305322.499197
ACGCCTA955.513175E-721.31732721
ATCGCCA855.0230483E-621.17570927
CCACGAG754.5924444E-521.00151420
TGGCGAT550.003931393420.4560222
ATTAAAT550.003933856720.45380832
GAGCGTA550.003933856720.45380832
ATAGTAC550.003933856720.4538083
CGCCATA550.003933856720.45380829
TATCAAC10250.019.9748922
ACGAGAC807.5463424E-519.6889222
Produced by FastQC (version 0.11.2)