FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 30h_57.341000000058ed.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 30h_57.341000000058ed.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences290327
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCCTCG207.025757E-444.998458
CTCGTAT301.1380518E-437.49870739
TCGTATG352.8157892E-432.1417540
GTGCAAG300.00513320530.0093061
TTCCGAA300.00514186229.99896620
GCCCACG406.1561953E-428.12403318
AAATCCG450.001223362925.00344724
CGTTAAA450.001224592424.9991413
ATACCGT450.001224592424.999146
TGCCGTC450.001224592424.9991445
CCGAAGT500.002258754822.50310128
CCCACGA500.002261017422.49922619
AATCTCG500.002261017422.49922636
GGATTTA500.002261017422.49922620
CGAGCCC602.4619247E-422.49922415
GTATAGT654.218882E-420.7756731
TGATACA550.00393034620.453844
TGCGGCT550.00393034620.4538415
GCGGCTT550.00393034620.4538416
GACTCAT550.00393034620.4538432
Produced by FastQC (version 0.11.2)