FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 30h_55.341000000058c3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 30h_55.341000000058c3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences476758
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGATCGTGTATCTCGTATGCCGTC10090.21163777010558818No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGCT207.030258E-444.998537
ATCGTGT1200.039.37784630
CTCGTAT1600.035.155139
TCGTGTA1400.033.748931
TATCTCG1650.032.72620436
CGTGTAT1450.032.58514432
TATGCCG1750.032.1418143
GATCGTG1500.031.50227729
TCGTATG1800.031.2489840
ACGATCG1600.030.93973527
GTATCTC1700.030.44018435
CGAGACG1700.030.44018423
CGTATGC1950.029.99902241
TCTCGTA1900.029.60429838
GTATCAA7100.028.2210621
TACACTA406.161984E-428.1240835
ACGAGAC2100.027.85623422
GACGATC1800.027.50198726
CGATCGT1750.027.00195128
GTATGCC2200.026.59004242
Produced by FastQC (version 0.11.2)