FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 30h_54.341000000058b6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 30h_54.341000000058b6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences490649
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTTGCG602.4656963E-422.4986742
GTATCAA7350.021.740061
TTGCGGT754.594205E-521.00090417
GTATGGG654.2264105E-420.774361
TTTGCGT654.235144E-420.768013
TAAGAGT1201.1021257E-820.6237854
ATCTCCG7000.020.25293545
CGGGGTG1555.311449E-1018.8717817
GCACCGT600.00649985918.7527186
GCCTAGT600.00650368618.75080512
CCCGAAC600.006507514518.7488963
CCGTGCG1354.2502506E-818.335998
CATCTCC16100.018.31010845
GTCTTAC750.001107154218.0044461
GGTATCA4250.018.0044441
CTCCGAG2900.017.84741645
CTTACAC901.8637674E-417.498973
GCGTACT1459.629366E-817.069712
CCACTAC800.001706136316.8791681
TGCGGTA952.8143005E-416.5796618
Produced by FastQC (version 0.11.2)