FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 30h_5.34100000005569.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 30h_5.34100000005569.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences412441
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAATGCGTTATCTCGTATGCCGTC4490.10886405570736178No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGGTG250.00210487736.0023325
TACCACG250.002106130135.9979634
TGGCGAT300.005141850630.0019422
GCCTAGG500.002262910622.49872835
TAACTGG500.002262910622.4987286
CATCTCC10400.022.28509545
TTATAGA654.2335002E-420.7680554
ACGGAAC654.2335002E-420.7680553
GTATCAA5500.020.4633121
ATTTATA550.00392809120.458352
TACCATA550.00392809120.458352
ACGCCTA1109.9384124E-820.45586821
CGCCTAA1109.9384124E-820.45586822
GCTGCGG550.003933613720.4533888
AACGCCT1202.4853216E-718.75121320
GATTACG600.00650570918.7489411
CGGAACA851.202587E-418.5283644
CAAAACG1354.249341E-818.33451817
AGGGTGT1002.0424626E-517.9989816
AAACGCC1253.820751E-717.99898119
Produced by FastQC (version 0.11.2)