FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 30h_48.3410000000585a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 30h_48.3410000000585a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences415776
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGATATAAATCTCGTATGCCGTC107942.596109443546525No Hit
ACTTAGAGCTGCATTCCCAAACAACTCGACTCTTCGAAGGAACTTTACATA28870.6943642730701147No Hit
GTCCCAGACCTATGTAAAGTTCCTTCGAAGAGTCGAGTTGTTTGGGAATGC17090.411038636188717No Hit
TGGTAGGAGAGCGTTCTAAGTGCGGTGAAGTCTAATCGAGAGGATAGATGG15710.37784768721619333No Hit
TCCCAGACCTATGTAAAGTTCCTTCGAAGAGTCGAGTTGTTTGGGAATGCA13100.31507350111598553No Hit
CTGTACTTGTTCGCTATCGGTCTCTCGCCAATATTTAGCTTTAGATGGAAT12720.305933964442392No Hit
CAGTAGGCTAATGGCGCATGTAACTGGAATCATGTGTAAGCGTCAAGGCTT11110.2672111906411144No Hit
TTCCCAAACAACTCGACTCTTCGAAGGAACTTTACATAGGTCTGGGACATC10100.24291926421919496No Hit
CCATCTATCCTCTCGATTAGACTTCACCGCACTTAGAACGCTCTCCTACCA9660.23233664280766564No Hit
GTACTTGTTCGCTATCGGTCTCTCGCCAATATTTAGCTTTAGATGGAATTT9040.21742476718232895No Hit
TTCCATCACTGTACTTGTTCGCTATCGGTCTCTCGCCAATATTTAGCTTTA8810.21189294235357503No Hit
GGGTGAGAATCCCGTGCGATGAGATGTCCCAGACCTATGTAAAGTTCCTTC8740.2102093434926499No Hit
CTTCTAAGCGCTCCATCTATCCTCTCGATTAGACTTCACCGCACTTAGAAC8740.2102093434926499No Hit
GAATTTACCACCCACTTAGAGCTGCATTCCCAAACAACTCGACTCTTCGAA8710.20948780112368198No Hit
CTTCAAGCCTTGACGCTTACACATGATTCCAGTTACATGCGCCATTAGCCT8610.20708265989378896No Hit
CTCTTTGAATGATGGCTGCTTCTAAGCCAACGTCCTAGTTGTCTACGCAAC8320.2001077503270992No Hit
GCTCTAAGTGGGTGGTAAATTCCATCTAAAGCTAAATATTGGCGAGAGACC8270.1989051797121527No Hit
CACCTATCTTTCGCTACTCATACCGGCATTCTCACTTCTAAGCGCTCCATC8040.19337335488339877No Hit
GTAGGAGAGCGTTCTAAGTGCGGTGAAGTCTAATCGAGAGGATAGATGGAG8010.19265181251443084No Hit
ACTTTATTTCGCTCCGCATCACGCCTCAGCCTTTTAGAAGCAAGCATTTGA7840.18856307242361273No Hit
TCACTGTACTTGTTCGCTATCGGTCTCTCGCCAATATTTAGCTTTAGATGG7760.1866389594396983No Hit
GTGTGACATCATGACCTTCGCTACTTTATTTCGCTCCGCATCACGCCTCAG7460.17942353575001924No Hit
CCTATGTAAAGTTCCTTCGAAGAGTCGAGTTGTTTGGGAATGCAGCTCTAA7400.17798045101208343No Hit
CCCACTTAGAGCTGCATTCCCAAACAACTCGACTCTTCGAAGGAACTTTAC7070.17004348495343646No Hit
GTGAGAATCCCGTGCGATGAGATGTCCCAGACCTATGTAAAGTTCCTTCGA6820.1640306318787039No Hit
CGCCAATATTTAGCTTTAGATGGAATTTACCACCCACTTAGAGCTGCATTC6710.1613849765258216No Hit
GAACGTCACAGAGGGTGAGAATCCCGTGCGATGAGATGTCCCAGACCTATG6340.15248595397521744No Hit
GCATAGTACCGAAACTGTGGATGTGCGAAAGCACGTGGTAGGAGAGCGTTC6270.1508023551142923No Hit
ACTCATACCGGCATTCTCACTTCTAAGCGCTCCATCTATCCTCTCGATTAG6100.14671361502347416No Hit
GGGCTAAGCATAGTACCGAAACTGTGGATGTGCGAAAGCACGTGGTAGGAG6070.14599207265450628No Hit
GCCTTTTAGAAGCAAGCATTTGACTCACTTCAAGCCTTGACGCTTACACAT6060.14575155853151697No Hit
ATTACGCACTCTTTGAATGATGGCTGCTTCTAAGCCAACGTCCTAGTTGTC5810.13973870545678443No Hit
CTATCGGTCTCTCGCCAATATTTAGCTTTAGATGGAATTTACCACCCACTT5670.1363715077349342No Hit
GTACCGAAACTGTGGATGTGCGAAAGCACGTGGTAGGAGAGCGTTCTAAGT5570.13396636650504118No Hit
CTTTGAATGATGGCTGCTTCTAAGCCAACGTCCTAGTTGTCTACGCAACTC5560.13372585238205187No Hit
CTTCTAAGCCAACGTCCTAGTTGTCTACGCAACTCCACATCCTTTTCCACT5410.13011814053721235No Hit
GGCTTGAAGTGAGTCAAATGCTTGCTTCTAAAAGGCTGAGGCGTGATGCGG5390.12963711229123373No Hit
CCATATATCTATACGCGGGAGTCAGACTGCGAGTGATAAGATCCGTAGTCG5390.12963711229123373No Hit
GTATCAACGCAGAGTACGGGTAATATCTCTCTGGCTAGAAGCTTTTCTCGG5370.12915608404525514No Hit
GTTTGGGAATGCAGCTCTAAGTGGGTGGTAAATTCCATCTAAAGCTAAATA5190.12482682983144769No Hit
CATCTATCCTCTCGATTAGACTTCACCGCACTTAGAACGCTCTCCTACCAC5050.12145963210959748No Hit
GGCTAATGGCGCATGTAACTGGAATCATGTGTAAGCGTCAAGGCTTGAAGT5030.12097860386361886No Hit
TCCTAGTTGTCTACGCAACTCCACATCCTTTTCCACTTAGCATAGATTTGG4830.11616832140383282No Hit
GCTTTAGATGGAATTTACCACCCACTTAGAGCTGCATTCCCAAACAACTCG4820.11592780728084354No Hit
CCCCAAATCTATGCTAAGTGGAAAAGGATGTGGAGTTGCGTAGACAACTAG4730.11376318017393983No Hit
GTCTAATCGAGAGGATAGATGGAGCGCTTAGAAGTGAGAATGCCGGTATGA4720.11352266605095052No Hit
GGATAGATGGAGCGCTTAGAAGTGAGAATGCCGGTATGAGTAGCGAAAGAT4700.11304163780497191No Hit
GGTCTGGGACATCTCATCGCACGGGATTCTCACCCTCTGTGACGTTCTGTT4680.1125606095589933No Hit
CTTCGAAGGAACTTTACATAGGTCTGGGACATCTCATCGCACGGGATTCTC4600.11063649657507889No Hit
ACTTTACATAGGTCTGGGACATCTCATCGCACGGGATTCTCACCCTCTGTG4570.10991495420611097No Hit
CTATTACGCACTCTTTGAATGATGGCTGCTTCTAAGCCAACGTCCTAGTTG4500.10823135534518587No Hit
GCTAAGCATAGTACCGAAACTGTGGATGTGCGAAAGCACGTGGTAGGAGAG4490.10799084122219656No Hit
CACTTAGAACGCTCTCCTACCACGTGCTTTCGCACATCCACAGTTTCGGTA4470.10750981297621795No Hit
TCTTGGAACAGAACGTCACAGAGGGTGAGAATCCCGTGCGATGAGATGTCC4260.10245901639344263No Hit
ATTTACCACCCACTTAGAGCTGCATTCCCAAACAACTCGACTCTTCGAAGG4230.10173747402447472No Hit
ATCATGACCTTCGCTACTTTATTTCGCTCCGCATCACGCCTCAGCCTTTTA4220.10149695990148541No Hit
GAACAGAACGTCACAGAGGGTGAGAATCCCGTGCGATGAGATGTCCCAGAC4160.1000538751635496No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC11150.042.7776445
ATATAAA10950.042.120731
TATAAAT10950.042.120732
GTATGCC11350.042.0238542
ATCTCGT11250.041.99741737
AATCTCG11000.041.9292436
TATGCCG11400.041.83953543
ATGCCGT11400.041.83953544
ACAGATA11200.041.79319827
GATATAA11100.041.76423330
CTCGTAT11350.041.62739639
CAGATAT11250.041.60745228
AAATCTC11000.041.52017635
TCGTATG11450.041.46033540
ATAAATC11200.040.97962633
TCTCGTA11600.040.7302538
CGAGACA11550.040.71178423
TAAATCT11300.040.6169734
CGAGCCC12000.040.5023815
CGTATGC11800.040.42124641
Produced by FastQC (version 0.11.2)