##FastQC	0.11.2
>>Basic Statistics	pass
#Measure	Value
Filename	H23NVBCXX l02n01 30h_47.3410000000584d.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1487
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	34.591123066577	38.0	32.0	38.0	32.0	38.0
2	35.1546738399462	38.0	32.0	38.0	32.0	38.0
3	35.11566913248151	38.0	32.0	38.0	32.0	38.0
4	35.076664425016816	38.0	32.0	38.0	32.0	38.0
5	35.39475453934096	38.0	32.0	38.0	32.0	38.0
6	37.069939475453936	38.0	38.0	40.0	32.0	40.0
7	36.477471418964356	38.0	38.0	40.0	32.0	40.0
8	36.22864828513786	38.0	38.0	40.0	32.0	40.0
9	36.75050437121722	38.0	38.0	40.0	32.0	40.0
10	36.45595158036315	38.0	38.0	40.0	32.0	40.0
11	36.723604572965705	38.0	38.0	40.0	32.0	40.0
12	36.09482178883658	38.0	38.0	40.0	32.0	40.0
13	36.367182246133154	38.0	38.0	40.0	32.0	40.0
14	36.49562878278413	38.0	38.0	40.0	32.0	40.0
15	36.75386684599866	38.0	38.0	40.0	32.0	40.0
16	35.94149293880296	38.0	38.0	40.0	27.0	40.0
17	36.03900470746469	38.0	38.0	40.0	27.0	40.0
18	35.85137861466039	38.0	38.0	40.0	27.0	40.0
19	35.9280430396772	38.0	38.0	40.0	27.0	40.0
20	36.128446536650976	38.0	38.0	40.0	32.0	40.0
21	36.53799596503026	38.0	38.0	40.0	32.0	40.0
22	36.36852723604573	38.0	38.0	40.0	32.0	40.0
23	36.5305985205111	38.0	38.0	40.0	32.0	40.0
24	36.32347007397445	38.0	38.0	40.0	32.0	40.0
25	35.96503026227303	38.0	38.0	40.0	32.0	40.0
26	36.06724949562878	38.0	38.0	40.0	32.0	40.0
27	35.86012104909213	38.0	38.0	40.0	27.0	40.0
28	36.062542030934765	38.0	38.0	40.0	32.0	40.0
29	36.111634162743776	38.0	38.0	40.0	27.0	40.0
30	35.76059179556153	38.0	38.0	40.0	27.0	40.0
31	35.7626092804304	38.0	38.0	40.0	27.0	40.0
32	35.79354404841963	38.0	38.0	40.0	27.0	40.0
33	35.95427034297243	38.0	38.0	40.0	27.0	40.0
34	35.76933422999328	38.0	38.0	40.0	27.0	40.0
35	35.79623402824479	38.0	38.0	40.0	27.0	40.0
36	35.99932750504371	38.0	38.0	40.0	27.0	40.0
37	35.44586415601883	38.0	32.0	40.0	27.0	40.0
38	35.44048419636853	38.0	32.0	40.0	27.0	40.0
39	35.357767316745125	38.0	38.0	40.0	27.0	40.0
40	35.289845326160055	38.0	32.0	40.0	27.0	40.0
41	35.53530598520511	38.0	38.0	40.0	27.0	40.0
42	35.10625420309348	38.0	32.0	40.0	27.0	40.0
43	35.64357767316745	38.0	38.0	40.0	27.0	40.0
44	35.23402824478816	38.0	32.0	40.0	27.0	40.0
45	35.26765299260256	38.0	32.0	40.0	27.0	40.0
46	34.77269670477472	38.0	32.0	40.0	27.0	40.0
47	34.72494956287828	38.0	32.0	40.0	27.0	40.0
48	34.71889710827169	38.0	32.0	40.0	27.0	40.0
49	34.577673167451245	38.0	32.0	40.0	27.0	40.0
50	34.50773369199731	38.0	32.0	40.0	13.0	40.0
51	34.08675184936113	38.0	32.0	38.0	13.0	40.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
2201	1	-1.4007936507936591
2201	2	3.1600529100529116
2201	3	-1.1058201058201078
2201	4	2.6441798941798993
2201	5	0.8095238095238173
2201	6	1.3769841269841265
2201	7	2.148148148148138
2201	8	2.041005291005291
2201	9	2.539682539682545
2201	10	2.8029100529100432
2201	11	0.47089947089947515
2201	12	2.544973544973544
2201	13	2.7195767195767218
2201	14	1.8822751322751259
2201	15	1.462962962962969
2201	16	2.525132275132272
2201	17	2.5211640211640187
2201	18	2.7037037037037095
2201	19	2.654761904761898
2201	20	0.3783068783068728
2201	21	2.2010582010581956
2201	22	2.1190476190476133
2201	23	2.929894179894184
2201	24	3.006613756613767
2201	25	-4.857142857142858
2201	26	2.869047619047606
2201	27	1.863756613756614
2201	28	1.4510582010581885
2201	29	2.824074074074062
2201	30	4.161375661375658
2201	31	0.45634920634920206
2201	32	3.595238095238088
2201	33	3.7142857142857153
2201	34	2.333333333333343
2201	35	3.4060846560846514
2201	36	4.105820105820115
2201	37	3.8399470899470955
2201	38	3.4576719576719555
2201	39	4.019841269841272
2201	40	3.8174603174603163
2201	41	3.294973544973537
2201	42	2.0753968253968296
2201	43	0.48015873015872046
2201	44	-0.0039682539682459606
2201	45	-4.523809523809522
2201	46	4.481481481481474
2201	47	1.900793650793645
2201	48	1.1640211640211575
2201	49	1.4510582010581956
2201	50	1.7552910052909994
2201	51	-5.534391534391535
1101	1	-0.40079365079365914
1101	2	3.1600529100529116
1101	3	-2.605820105820108
1101	4	-2.8558201058201007
1101	5	-0.1904761904761827
1101	6	2.3769841269841265
1101	7	-1.5185185185185262
1101	8	-0.6256613756613731
1101	9	2.8730158730158806
1101	10	0.13624338624337895
1101	11	2.804232804232811
1101	12	1.87830687830688
1101	13	0.05291005291005746
1101	14	2.2156084656084616
1101	15	2.462962962962969
1101	16	3.525132275132272
1101	17	3.5211640211640187
1101	18	3.7037037037037095
1101	19	-0.011904761904766303
1101	20	-0.2883597883597915
1101	21	2.5343915343915313
1101	22	-0.547619047619051
1101	23	2.5965608465608483
1101	24	-0.32671957671956875
1101	25	-8.69047619047619
1101	26	-2.297619047619058
1101	27	2.1970899470899496
1101	28	2.4510582010581885
1101	29	-9.342592592592602
1101	30	-2.338624338624342
1101	31	3.456349206349202
1101	32	3.9285714285714235
1101	33	0.3809523809523796
1101	34	-9.166666666666657
1101	35	-2.4272486772486843
1101	36	-1.7275132275132208
1101	37	-1.3267195767195687
1101	38	-8.708994708994709
1101	39	2.6865079365079367
1101	40	2.4841269841269806
1101	41	-8.871693121693127
1101	42	0.7420634920634939
1101	43	3.4801587301587205
1101	44	-8.503968253968246
1101	45	-0.6904761904761898
1101	46	-7.6851851851851904
1101	47	4.234126984126981
1101	48	4.1640211640211575
1101	49	4.451058201058196
1101	50	-7.744708994709001
1101	51	-0.367724867724867
2202	1	2.599206349206341
2202	2	3.1600529100529116
2202	3	-5.43915343915344
2202	4	2.6441798941798993
2202	5	0.8095238095238173
2202	6	2.043650793650791
2202	7	2.8148148148148096
2202	8	2.041005291005291
2202	9	3.2063492063492163
2202	10	3.4695767195767147
2202	11	0.47089947089947515
2202	12	2.544973544973544
2202	13	3.386243386243393
2202	14	2.5489417989417973
2202	15	0.7962962962963047
2202	16	3.191798941798936
2202	17	3.8544973544973544
2202	18	4.037037037037045
2202	19	2.654761904761898
2202	20	2.378306878306873
2202	21	0.867724867724867
2202	22	2.1190476190476133
2202	23	2.2632275132275126
2202	24	3.006613756613767
2202	25	4.80952380952381
2202	26	4.202380952380942
2202	27	2.5304232804232853
2202	28	2.117724867724853
2202	29	2.824074074074062
2202	30	2.828042328042322
2202	31	3.1230158730158664
2202	32	4.261904761904759
2202	33	1.7142857142857153
2202	34	3.6666666666666785
2202	35	-5.593915343915352
2202	36	-0.22751322751322078
2202	37	0.17328042328043125
2202	38	2.124338624338627
2202	39	4.019841269841272
2202	40	3.8174603174603163
2202	41	1.2949735449735371
2202	42	3.408730158730158
2202	43	3.1468253968253848
2202	44	3.6626984126984183
2202	45	5.142857142857146
2202	46	5.148148148148145
2202	47	3.900793650793645
2202	48	4.497354497354493
2202	49	5.451058201058196
2202	50	4.421957671957664
2202	51	4.132275132275133
1102	1	-0.40079365079365914
1102	2	3.1600529100529116
1102	3	-0.10582010582010781
1102	4	2.6441798941798993
1102	5	2.8095238095238173
1102	6	3.3769841269841265
1102	7	2.481481481481474
1102	8	1.3743386243386269
1102	9	-1.1269841269841194
1102	10	2.136243386243379
1102	11	-10.69576719576719
1102	12	-3.62169312169312
1102	13	3.0529100529100575
1102	14	2.2156084656084616
1102	15	2.462962962962969
1102	16	0.5251322751322718
1102	17	3.5211640211640187
1102	18	-0.2962962962962905
1102	19	1.9880952380952337
1102	20	1.7116402116402085
1102	21	1.5343915343915313
1102	22	1.452380952380949
1102	23	-1.4034391534391517
1102	24	1.6732804232804313
1102	25	0.8095238095238102
1102	26	2.202380952380942
1102	27	1.1970899470899496
1102	28	2.4510582010581885
1102	29	3.1574074074073977
1102	30	-10.338624338624342
1102	31	-10.043650793650798
1102	32	-3.0714285714285765
1102	33	2.3809523809523796
1102	34	2.333333333333343
1102	35	3.0727513227513157
1102	36	2.772486772486779
1102	37	3.1732804232804313
1102	38	3.791005291005291
1102	39	-9.813492063492063
1102	40	2.4841269841269806
1102	41	2.628306878306873
1102	42	3.742063492063494
1102	43	3.4801587301587205
1102	44	-0.0039682539682459606
1102	45	0.8095238095238102
1102	46	-8.68518518518519
1102	47	-1.2658730158730194
1102	48	-2.3359788359788425
1102	49	1.4510582010581956
1102	50	0.7552910052909994
1102	51	-1.867724867724867
2203	1	-0.40079365079365914
2203	2	3.1600529100529116
2203	3	2.894179894179892
2203	4	2.6441798941798993
2203	5	-0.1904761904761827
2203	6	3.3769841269841265
2203	7	2.481481481481474
2203	8	-10.125661375661373
2203	9	2.8730158730158806
2203	10	-2.363756613756621
2203	11	-0.19576719576718915
2203	12	2.87830687830688
2203	13	2.0529100529100575
2203	14	1.2156084656084616
2203	15	2.462962962962969
2203	16	2.525132275132272
2203	17	-2.9788359788359813
2203	18	-0.2962962962962905
2203	19	1.9880952380952337
2203	20	1.7116402116402085
2203	21	2.5343915343915313
2203	22	3.452380952380949
2203	23	3.5965608465608483
2203	24	2.6732804232804313
2203	25	0.8095238095238102
2203	26	-0.7976190476190581
2203	27	1.1970899470899496
2203	28	2.4510582010581885
2203	29	3.1574074074073977
2203	30	3.161375661375658
2203	31	3.456349206349202
2203	32	-1.5714285714285765
2203	33	-0.6190476190476204
2203	34	2.333333333333343
2203	35	-0.9272486772486843
2203	36	-0.22751322751322078
2203	37	-2.8267195767195687
2203	38	2.791005291005291
2203	39	2.6865079365079367
2203	40	2.4841269841269806
2203	41	2.628306878306873
2203	42	-0.25793650793650613
2203	43	2.4801587301587205
2203	44	4.996031746031754
2203	45	5.80952380952381
2203	46	3.8148148148148096
2203	47	5.234126984126981
2203	48	5.1640211640211575
2203	49	-2.0489417989418044
2203	50	4.755291005290999
2203	51	-8.367724867724867
1103	1	0.5992063492063409
1103	2	1.1600529100529116
1103	3	-3.105820105820108
1103	4	-5.689153439153433
1103	5	-11.190476190476183
1103	6	-0.6230158730158735
1103	7	2.148148148148138
1103	8	1.3743386243386269
1103	9	-6.460317460317452
1103	10	0.8029100529100432
1103	11	2.470899470899475
1103	12	2.544973544973544
1103	13	-1.6137566137566068
1103	14	1.2156084656084616
1103	15	-0.537037037037031
1103	16	2.525132275132272
1103	17	2.5211640211640187
1103	18	2.7037037037037095
1103	19	-0.011904761904766303
1103	20	-0.2883597883597915
1103	21	-0.4656084656084687
1103	22	-0.547619047619051
1103	23	-2.070105820105816
1103	24	-0.32671957671956875
1103	25	2.80952380952381
1103	26	-5.464285714285726
1103	27	-2.8029100529100504
1103	28	-8.882275132275144
1103	29	-5.50925925925927
1103	30	-1.5052910052910065
1103	31	-14.210317460317466
1103	32	-9.071428571428577
1103	33	-9.952380952380953
1103	34	-9.666666666666657
1103	35	-10.260582010582016
1103	36	-5.560846560846553
1103	37	-5.160052910052901
1103	38	-5.542328042328041
1103	39	-0.31349206349206327
1103	40	-5.8492063492063515
1103	41	-1.0383597883597915
1103	42	-5.591269841269838
1103	43	-5.853174603174612
1103	44	-5.337301587301578
1103	45	-4.523809523809522
1103	46	0.8148148148148096
1103	47	-6.432539682539687
1103	48	0.16402116402115752
1103	49	4.11772486772486
1103	50	-3.9113756613756685
1103	51	-0.867724867724867
2204	1	-2.900793650793659
2204	2	-2.3399470899470884
2204	3	-0.10582010582010781
2204	4	-0.3558201058201007
2204	5	-2.6904761904761827
2204	6	-3.1230158730158735
2204	7	-10.018518518518526
2204	8	-3.125661375661373
2204	9	-2.6269841269841194
2204	10	-0.863756613756621
2204	11	-10.69576719576719
2204	12	-10.62169312169312
2204	13	-10.447089947089943
2204	14	-11.284391534391538
2204	15	-1.537037037037031
2204	16	-12.974867724867728
2204	17	-2.9788359788359813
2204	18	2.7037037037037095
2204	19	1.9880952380952337
2204	20	-4.2883597883597915
2204	21	-1.4656084656084687
2204	22	-4.547619047619051
2204	23	-3.9034391534391517
2204	24	-3.8267195767195687
2204	25	-9.69047619047619
2204	26	-3.797619047619058
2204	27	-1.8029100529100504
2204	28	-1.5489417989418115
2204	29	2.1574074074073977
2204	30	-3.838624338624342
2204	31	-6.043650793650798
2204	32	-9.571428571428577
2204	33	-22.61904761904762
2204	34	-3.166666666666657
2204	35	-3.9272486772486843
2204	36	-3.227513227513221
2204	37	-9.326719576719569
2204	38	-2.708994708994709
2204	39	2.6865079365079367
2204	40	-3.0158730158730194
2204	41	-2.871693121693127
2204	42	-2.757936507936506
2204	43	2.4801587301587205
2204	44	-0.0039682539682459606
2204	45	-14.19047619047619
2204	46	0.8148148148148096
2204	47	0.23412698412698063
2204	48	-2.8359788359788425
2204	49	-2.0489417989418044
2204	50	-8.744708994709
2204	51	-11.367724867724867
2205	1	-3.400793650793659
2205	2	-2.8399470899470884
2205	3	-3.105820105820108
2205	4	2.6441798941798993
2205	5	2.8095238095238173
2205	6	2.3769841269841265
2205	7	-1.5185185185185262
2205	8	-1.6256613756613731
2205	9	1.8730158730158806
2205	10	2.136243386243379
2205	11	2.804232804232811
2205	12	-1.12169312169312
2205	13	2.0529100529100575
2205	14	1.2156084656084616
2205	15	-11.037037037037031
2205	16	-9.974867724867728
2205	17	-5.978835978835981
2205	18	-0.2962962962962905
2205	19	-2.5119047619047663
2205	20	-0.2883597883597915
2205	21	-3.9656084656084687
2205	22	-1.547619047619051
2205	23	-1.4034391534391517
2205	24	2.6732804232804313
2205	25	-0.1904761904761898
2205	26	-6.297619047619058
2205	27	-1.8029100529100504
2205	28	-4.5489417989418115
2205	29	-0.8425925925926023
2205	30	-10.338624338624342
2205	31	-10.043650793650798
2205	32	-2.5714285714285765
2205	33	-6.11904761904762
2205	34	-10.166666666666657
2205	35	-10.427248677248684
2205	36	-2.727513227513221
2205	37	-12.326719576719569
2205	38	-5.708994708994709
2205	39	-0.31349206349206327
2205	40	2.4841269841269806
2205	41	-0.3716931216931272
2205	42	-0.25793650793650613
2205	43	3.4801587301587205
2205	44	2.996031746031754
2205	45	0.8095238095238102
2205	46	-8.68518518518519
2205	47	-2.2658730158730194
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1209	13	0.7195767195767218
1209	14	1.8822751322751259
1209	15	0.12962962962963331
1209	16	-5.141534391534396
1209	17	3.187830687830683
1209	18	3.370370370370374
1209	19	1.3214285714285694
1209	20	2.378306878306873
1209	21	2.2010582010581956
1209	22	0.1190476190476133
1209	23	2.2632275132275126
1209	24	2.3399470899470955
1209	25	1.4761904761904745
1209	26	2.869047619047606
1209	27	1.863756613756614
1209	28	2.784391534391524
1209	29	3.4907407407407334
1209	30	-7.50529100529101
1209	31	3.1230158730158664
1209	32	1.5952380952380878
1209	33	3.047619047619044
1209	34	3.000000000000007
1209	35	3.4060846560846514
1209	36	3.4391534391534435
1209	37	3.8399470899470955
1209	38	3.4576719576719555
1209	39	3.353174603174601
1209	40	3.150793650793645
1209	41	3.294973544973537
1209	42	3.408730158730158
1209	43	-0.5198412698412795
1209	44	-0.0039682539682459606
1209	45	2.4761904761904745
1209	46	0.48148148148147385
1209	47	-4.432539682539687
1209	48	-0.16931216931217818
1209	49	2.11772486772486
1209	50	2.4219576719576636
1209	51	4.798941798941797
1214	1	-3.400793650793659
1214	2	-2.3399470899470884
1214	3	2.894179894179892
1214	4	2.6441798941798993
1214	5	-0.1904761904761827
1214	6	1.3769841269841265
1214	7	-0.5185185185185262
1214	8	-1.6256613756613731
1214	9	-9.62698412698412
1214	10	-10.363756613756621
1214	11	-0.19576719576718915
1214	12	1.87830687830688
1214	13	-0.9470899470899425
1214	14	2.2156084656084616
1214	15	1.462962962962969
1214	16	-3.474867724867728
1214	17	-0.4788359788359813
1214	18	2.7037037037037095
1214	19	-10.511904761904766
1214	20	1.7116402116402085
1214	21	-1.4656084656084687
1214	22	2.452380952380949
1214	23	2.5965608465608483
1214	24	-4.326719576719569
1214	25	-0.1904761904761898
1214	26	-0.7976190476190581
1214	27	-1.8029100529100504
1214	28	1.4510582010581885
1214	29	2.1574074074073977
1214	30	-10.338624338624342
1214	31	-0.5436507936507979
1214	32	-9.571428571428577
1214	33	-10.11904761904762
1214	34	-3.166666666666657
1214	35	-2.4272486772486843
1214	36	-9.72751322751322
1214	37	-2.3267195767195687
1214	38	-8.708994708994709
1214	39	-1.8134920634920633
1214	40	-2.0158730158730194
1214	41	-1.8716931216931272
1214	42	-2.757936507936506
1214	43	-10.01984126984128
1214	44	-9.503968253968246
1214	45	-1.6904761904761898
1214	46	-8.68518518518519
1214	47	-9.26587301587302
1214	48	-2.3359788359788425
1214	49	-8.048941798941804
1214	50	-8.744708994709
1214	51	-7.367724867724867
1215	1	-1.0674603174603234
1215	2	3.1600529100529116
1215	3	0.8941798941798922
1215	4	-3.022486772486765
1215	5	-5.523809523809515
1215	6	-1.6230158730158735
1215	7	2.8148148148148096
1215	8	-0.9589947089947088
1215	9	-5.126984126984119
1215	10	3.4695767195767147
1215	11	-5.195767195767189
1215	12	2.544973544973544
1215	13	-13.947089947089943
1215	14	-1.7843915343915384
1215	15	-5.203703703703699
1215	16	-7.141534391534396
1215	17	-7.145502645502649
1215	18	-0.2962962962962905
1215	19	-3.0119047619047663
1215	20	1.7116402116402085
1215	21	-0.4656084656084687
1215	22	2.1190476190476133
1215	23	2.2632275132275126
1215	24	2.3399470899470955
1215	25	3.4761904761904745
1215	26	3.5357142857142776
1215	27	2.5304232804232853
1215	28	2.784391534391524
1215	29	3.4907407407407334
1215	30	3.4947089947089935
1215	31	0.45634920634920206
1215	32	2.261904761904759
1215	33	3.7142857142857153
1215	34	3.000000000000007
1215	35	2.73941798941798
1215	36	1.4391534391534435
1215	37	0.17328042328043125
1215	38	-2.875661375661373
1215	39	-7.646825396825395
1215	40	-7.8492063492063515
1215	41	-5.038359788359795
1215	42	-0.25793650793650613
1215	43	-5.186507936507947
1215	44	-5.337301587301578
1215	45	-4.523809523809522
1215	46	-4.518518518518523
1215	47	-4.432539682539687
1215	48	-7.169312169312175
1215	49	0.4510582010581956
1215	50	0.7552910052909994
1215	51	-3.534391534391535
1212	1	2.599206349206341
1212	2	3.1600529100529116
1212	3	2.894179894179892
1212	4	2.6441798941798993
1212	5	-3.1904761904761827
1212	6	1.3769841269841265
1212	7	1.4814814814814738
1212	8	3.374338624338627
1212	9	1.8730158730158806
1212	10	4.136243386243379
1212	11	3.804232804232811
1212	12	3.87830687830688
1212	13	4.0529100529100575
1212	14	3.2156084656084616
1212	15	3.462962962962969
1212	16	4.525132275132272
1212	17	2.5211640211640187
1212	18	2.7037037037037095
1212	19	1.9880952380952337
1212	20	1.7116402116402085
1212	21	3.5343915343915313
1212	22	3.452380952380949
1212	23	3.5965608465608483
1212	24	1.6732804232804313
1212	25	2.80952380952381
1212	26	2.202380952380942
1212	27	3.1970899470899496
1212	28	3.4510582010581885
1212	29	4.157407407407398
1212	30	4.161375661375658
1212	31	2.456349206349202
1212	32	2.9285714285714235
1212	33	2.3809523809523796
1212	34	2.333333333333343
1212	35	2.0727513227513157
1212	36	2.772486772486779
1212	37	5.173280423280431
1212	38	4.791005291005291
1212	39	4.686507936507937
1212	40	4.484126984126981
1212	41	4.628306878306873
1212	42	4.742063492063494
1212	43	4.4801587301587205
1212	44	2.996031746031754
1212	45	5.80952380952381
1212	46	5.8148148148148096
1212	47	5.234126984126981
1212	48	5.1640211640211575
1212	49	3.4510582010581956
1212	50	3.7552910052909994
1212	51	4.132275132275133
1213	1	2.599206349206341
1213	2	3.1600529100529116
1213	3	2.894179894179892
1213	4	2.6441798941798993
1213	5	2.8095238095238173
1213	6	1.3769841269841265
1213	7	1.4814814814814738
1213	8	3.374338624338627
1213	9	1.8730158730158806
1213	10	4.136243386243379
1213	11	-4.195767195767189
1213	12	1.87830687830688
1213	13	-3.9470899470899425
1213	14	-4.784391534391538
1213	15	1.462962962962969
1213	16	-3.474867724867728
1213	17	-3.4788359788359813
1213	18	-3.2962962962962905
1213	19	1.9880952380952337
1213	20	-4.2883597883597915
1213	21	1.5343915343915313
1213	22	1.452380952380949
1213	23	-4.403439153439152
1213	24	1.6732804232804313
1213	25	-3.19047619047619
1213	26	2.202380952380942
1213	27	1.1970899470899496
1213	28	-4.5489417989418115
1213	29	-8.842592592592602
1213	30	2.161375661375658
1213	31	-22.543650793650798
1213	32	2.9285714285714235
1213	33	2.3809523809523796
1213	34	2.333333333333343
1213	35	4.072751322751316
1213	36	2.772486772486779
1213	37	3.1732804232804313
1213	38	2.791005291005291
1213	39	2.6865079365079367
1213	40	2.4841269841269806
1213	41	2.628306878306873
1213	42	2.742063492063494
1213	43	4.4801587301587205
1213	44	2.996031746031754
1213	45	5.80952380952381
1213	46	5.8148148148148096
1213	47	3.2341269841269806
1213	48	3.1640211640211575
1213	49	3.4510582010581956
1213	50	3.7552910052909994
1213	51	-1.867724867724867
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
23	3.0
24	5.0
25	12.0
26	19.0
27	17.0
28	32.0
29	47.0
30	45.0
31	68.0
32	76.0
33	84.0
34	112.0
35	104.0
36	140.0
37	178.0
38	272.0
39	273.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.157469717362048	12.85329744279946	9.757738896366083	46.23149394347241
2	11.903160726294553	15.534633490248822	51.5803631472764	20.98184263618023
3	14.794889038332212	19.83860121049092	46.40215198386012	18.964357767316745
4	10.087424344317418	37.65971755211836	27.50504371217216	24.747814391392065
5	9.818426361802286	27.50504371217216	49.630127774041696	13.04640215198386
6	23.67182246133154	49.29388029589778	13.180901143241424	13.853396099529252
7	19.031607262945528	26.832548755884332	15.601882985877605	38.53396099529254
8	17.753866845998655	51.1768661735037	14.996637525218562	16.072629455279085
9	19.30060524546066	15.534633490248822	14.122394082044384	51.04236718224613
10	11.36516476126429	43.77942165433759	23.470073974445192	21.385339609952926
11	24.6133154001345	18.695359784801614	40.08069939475454	16.610625420309347
12	15.66913248150639	19.973100201748487	24.277067921990586	40.08069939475454
13	22.19233355749832	15.534633490248822	41.08944182918628	21.183591123066577
14	16.47612642905178	17.821116341627437	19.56960322797579	46.13315400134499
15	19.031607262945528	20.107599193006052	19.30060524546066	41.56018829858776
16	38.93745796906523	21.856086079354405	18.291862811028917	20.914593140551446
17	19.166106254203093	42.23268325487559	20.51109616677875	18.09011432414257
18	41.425689307330195	21.385339609952926	20.3093476798924	16.87962340282448
19	19.36785474108944	21.04909213180901	18.62811028917283	40.95494283792871
20	18.426361802286483	20.847343644922663	20.3093476798924	40.41694687289845
21	17.88836583725622	21.654337592468057	19.36785474108944	41.08944182918628
22	16.812373907195695	44.720914593140556	20.578345662407532	17.88836583725622
23	16.94687289845326	21.788836583725622	19.636852723604573	41.62743779421654
24	37.794216543375924	23.67182246133154	19.704102219233356	18.82985877605918
25	18.897108271687962	42.972427706792196	19.636852723604573	18.493611297915265
26	39.071956960322794	22.595830531271016	21.587088096839274	16.745124411566913
27	17.61936785474109	45.66240753194351	18.897108271687962	17.821116341627437
28	14.862138533960994	21.788836583725622	21.45258910558171	41.89643577673167
29	29.05178211163416	27.37054472091459	26.227303295225283	17.350369872225958
30	18.493611297915265	32.817753866846	30.060524546065903	18.62811028917283
31	21.45258910558171	22.259583053127102	27.101546738399463	29.186281102891726
32	19.233355749831876	28.04303967720242	32.4142568930733	20.3093476798924
33	19.56960322797579	23.40282447881641	39.004707464694015	18.022864828513786
34	19.83860121049092	30.396772024209817	24.747814391392065	25.016812373907193
35	27.37054472091459	22.19233355749832	24.747814391392065	25.68930733019502
36	14.660390047074648	23.40282447881641	43.3759246805649	18.560860793544048
37	15.46738399462004	41.761936785474106	25.353059852051107	17.41761936785474
38	14.52589105581708	19.233355749831876	46.53665097511769	19.704102219233356
39	15.534633490248822	17.484868863483523	24.34431741761937	42.636180228648286
40	13.382649630127775	18.426361802286483	47.61264290517821	20.578345662407532
41	17.014122394082047	17.552118359112306	25.285810356422328	40.147948890383326
42	36.3147276395427	18.3591123066577	26.832548755884332	18.493611297915265
43	14.929388029589777	18.897108271687962	47.209145931405516	18.964357767316745
44	11.903160726294553	39.071956960322794	28.98453261600538	20.04034969737727
45	13.180901143241424	19.233355749831876	47.881640887693344	19.704102219233356
46	35.373234700739744	17.081371889710827	26.29455279085407	21.25084061869536
47	13.651647612642906	17.821116341627437	26.563550773369197	41.96368527236046
48	12.911903160726295	20.376597175521184	22.595830531271016	44.11566913248151
49	33.01950235373235	17.753866845998655	26.967047747141898	22.259583053127102
50	11.36516476126429	20.376597175521184	46.26765299260256	21.99058507061197
51	9.41492938802959	17.350369872225958	27.706792199058505	45.52790854068595
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1.0
1	1.0
2	1.0
3	0.5
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.5
12	1.0
13	1.0
14	1.0
15	2.5
16	4.0
17	3.5
18	3.0
19	2.0
20	1.0
21	4.0
22	7.0
23	6.0
24	5.0
25	5.5
26	5.0
27	4.0
28	5.5
29	7.0
30	12.5
31	18.0
32	22.0
33	26.0
34	34.5
35	43.0
36	53.5
37	64.0
38	68.0
39	72.0
40	76.0
41	80.0
42	88.5
43	97.0
44	103.0
45	109.0
46	133.0
47	157.0
48	149.5
49	142.0
50	239.0
51	336.0
52	243.5
53	151.0
54	108.5
55	66.0
56	47.0
57	28.0
58	24.5
59	21.0
60	17.0
61	13.0
62	8.0
63	3.0
64	4.5
65	6.0
66	5.0
67	4.0
68	3.5
69	3.0
70	3.0
71	3.0
72	2.5
73	2.0
74	1.0
75	1.0
76	2.0
77	1.0
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.06724949562878278
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1487.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	79.35440484196369
#Duplication Level	Percentage of deduplicated	Percentage of total
1	98.38983050847457	78.07666442501682
2	0.3389830508474576	0.5379959650302623
3	0.2542372881355932	0.605245460659045
4	0.2542372881355932	0.8069939475453934
5	0.0	0.0
6	0.0847457627118644	0.4034969737726967
7	0.0	0.0
8	0.0	0.0
9	0.0	0.0
>10	0.5932203389830508	11.499663752521856
>50	0.0	0.0
>100	0.0847457627118644	8.069939475453934
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACGTCTTAGTATCTCGTATGCCGTC	120	8.069939475453934	No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGTC	41	2.757229320780094	No Hit
CTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGTC	31	2.0847343644922667	No Hit
CTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGTC	28	1.882985877605918	No Hit
CTTATACACATCTCCGAGCCCACGAGACAGTGTCTTATCTCGTATGCCGTC	25	1.6812373907195695	No Hit
CTTATACACATCTCCGAGCCCACGAGACGATCGTGTATCTCGTATGCCGTC	22	1.4794889038332213	No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGTC	12	0.8069939475453934	No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGTC	12	0.8069939475453934	No Hit
CTTATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGTC	6	0.4034969737726967	TruSeq Adapter, Index 12 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACGAATTGCTATCTCGTATGCCGTC	4	0.26899798251513113	RNA PCR Primer, Index 27 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACTCAACTGTATCTCGTATGCCGTC	4	0.26899798251513113	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4	0.26899798251513113	No Hit
TCTTATACACATCTCCGAGCCCACGAGACAGTGTCTTATCTCGTATGCCGT	3	0.20174848688634836	No Hit
TTATACACATCTCCGAGCCCACGAGACGTCTTAGTATCTCGTATGCCGTCT	3	0.20174848688634836	No Hit
CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3	0.20174848688634836	No Hit
CTTATACACATCTCCGAGCCCAGAGACAACATAATATCTCGTATGCCGTCT	2	0.13449899125756556	RNA PCR Primer, Index 44 (100% over 21bp)
TATCAACGCAGAGTACTTTTTTTTTTTTGCGAAGCAGTGGTATCAACGCAG	2	0.13449899125756556	No Hit
TTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGTCT	2	0.13449899125756556	No Hit
GCGTTGATACCACTGCTTCGCAAAAAAAAAAAAGTACTCTGCGTTGATACC	2	0.13449899125756556	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA Adapter	Nextera Transposase Sequence
1	0.0	0.0	0.0
2	0.0	0.0	0.0
3	0.0	0.0	0.0
4	0.0	0.0	0.0
5	0.0	0.0	0.0
6	0.0	0.0	0.0
7	0.0	0.0	0.0
8	0.0	0.0	0.0
9	0.0	0.0	0.0
10	0.0	0.0	0.0
11	0.0	0.0	0.0
12	0.0	0.0	0.0
13	0.0	0.0	0.0
14	0.0	0.0	0.0
15	0.0	0.0	0.0
16	0.0	0.0	0.0
17	0.0	0.0	0.0
18	0.0	0.0	0.0
19	0.0	0.0	0.0
20	0.0	0.0	0.06724949562878278
21	0.0	0.0	0.06724949562878278
22	0.0	0.0	0.13449899125756556
23	0.0	0.0	0.13449899125756556
24	0.0	0.0	0.13449899125756556
25	0.0	0.0	0.13449899125756556
26	0.0	0.0	0.26899798251513113
27	0.0	0.0	0.3362474781439139
28	0.0	0.0	0.47074646940147946
29	0.0	0.0	0.9414929388029589
30	0.0	0.0	1.3449899125756557
31	0.0	0.0	2.0174848688634834
32	0.0	0.0	2.9589778076664426
33	0.0	0.0	4.303967720242098
34	0.0	0.0	5.78345662407532
35	0.0	0.0	6.99394754539341
36	0.0	0.0	8.338937457969065
37	0.0	0.0	10.1546738399462
38	0.0	0.0	11.499663752521856
39	0.0	0.0	14.4586415601883
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCTC	25	0.0014878942	36.0	35
CGAGACG	25	0.0014878942	36.0	23
AGCCCAC	35	1.6675342E-4	32.14286	17
GTATGCC	35	1.6675342E-4	32.14286	42
ATCTCGT	35	1.6675342E-4	32.14286	37
ATCTCCG	35	1.6675342E-4	32.14286	10
CACATCT	35	1.6675342E-4	32.14286	7
GCCCACG	35	1.6675342E-4	32.14286	18
TGCCGTC	35	1.6675342E-4	32.14286	45
CCACGAG	35	1.6675342E-4	32.14286	20
TATGCCG	35	1.6675342E-4	32.14286	43
GAGCCCA	35	1.6675342E-4	32.14286	16
CACGAGA	35	1.6675342E-4	32.14286	21
CCCACGA	35	1.6675342E-4	32.14286	19
ACACATC	35	1.6675342E-4	32.14286	6
TCTCGTA	35	1.6675342E-4	32.14286	38
ACGAGAC	35	1.6675342E-4	32.14286	22
TATCTCG	35	1.6675342E-4	32.14286	36
ATGCCGT	35	1.6675342E-4	32.14286	44
CGTATGC	35	1.6675342E-4	32.14286	41
>>END_MODULE