FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 30h_47.3410000000584d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 30h_47.3410000000584d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1487
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTCTTAGTATCTCGTATGCCGTC1208.069939475453934No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGTC412.757229320780094No Hit
CTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGTC312.0847343644922667No Hit
CTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGTC281.882985877605918No Hit
CTTATACACATCTCCGAGCCCACGAGACAGTGTCTTATCTCGTATGCCGTC251.6812373907195695No Hit
CTTATACACATCTCCGAGCCCACGAGACGATCGTGTATCTCGTATGCCGTC221.4794889038332213No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGTC120.8069939475453934No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGTC120.8069939475453934No Hit
CTTATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGTC60.4034969737726967TruSeq Adapter, Index 12 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACGAATTGCTATCTCGTATGCCGTC40.26899798251513113RNA PCR Primer, Index 27 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACTCAACTGTATCTCGTATGCCGTC40.26899798251513113No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40.26899798251513113No Hit
TCTTATACACATCTCCGAGCCCACGAGACAGTGTCTTATCTCGTATGCCGT30.20174848688634836No Hit
TTATACACATCTCCGAGCCCACGAGACGTCTTAGTATCTCGTATGCCGTCT30.20174848688634836No Hit
CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30.20174848688634836No Hit
CTTATACACATCTCCGAGCCCAGAGACAACATAATATCTCGTATGCCGTCT20.13449899125756556RNA PCR Primer, Index 44 (100% over 21bp)
TATCAACGCAGAGTACTTTTTTTTTTTTGCGAAGCAGTGGTATCAACGCAG20.13449899125756556No Hit
TTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGTCT20.13449899125756556No Hit
GCGTTGATACCACTGCTTCGCAAAAAAAAAAAAGTACTCTGCGTTGATACC20.13449899125756556No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCTC250.001487894236.035
CGAGACG250.001487894236.023
AGCCCAC351.6675342E-432.1428617
GTATGCC351.6675342E-432.1428642
ATCTCGT351.6675342E-432.1428637
ATCTCCG351.6675342E-432.1428610
CACATCT351.6675342E-432.142867
GCCCACG351.6675342E-432.1428618
TGCCGTC351.6675342E-432.1428645
CCACGAG351.6675342E-432.1428620
TATGCCG351.6675342E-432.1428643
GAGCCCA351.6675342E-432.1428616
CACGAGA351.6675342E-432.1428621
CCCACGA351.6675342E-432.1428619
ACACATC351.6675342E-432.142866
TCTCGTA351.6675342E-432.1428638
ACGAGAC351.6675342E-432.1428622
TATCTCG351.6675342E-432.1428636
ATGCCGT351.6675342E-432.1428644
CGTATGC351.6675342E-432.1428641
Produced by FastQC (version 0.11.2)