FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 30h_46.34100000005830.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 30h_46.34100000005830.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences376897
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATACGT250.002104401436.00318524
GAATACG352.8153416E-432.14570223
ATCGATT300.00514069630.00265316
TATGCCG453.4857374E-529.99867243
ATGCCGT453.4857374E-529.99867244
CTATACT507.148004E-526.9988064
TAGGACA709.2833216E-725.713154
TGCCGTC551.3662306E-424.5443745
CTTTATA500.002260644722.501991
GCCCACG654.2322153E-420.7683118
ACGTAGG654.2322153E-420.7683127
CTACTAA550.003929689520.4563561
CTCTAAT550.003929689520.4563561
TATCATG550.003932711720.4536427
ATATAGG550.003932711720.4536422
GGACCTA550.003932711720.4536426
ACGAGAC706.967403E-419.2874222
CTCGTAT706.9736294E-419.28486339
CTAAGAC600.00650422818.7491723
GCCATAC600.00650422818.7491723
Produced by FastQC (version 0.11.2)