FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 30h_45.34100000005823.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 30h_45.34100000005823.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences437518
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTCGAG406.161182E-428.12407121
TATGCCG507.150045E-526.9991143
TAGACAG709.288142E-725.7134385
ACTAGAC450.001225576324.9991743
TACCGTG450.001225576324.9991747
ACGGGAT1001.3405952E-924.74918416
CGGGATG551.3666181E-424.54464317
GTATCAA7800.022.5146941
GGTATCA4050.022.2367361
CGGCGAC550.00393345420.4538699
ATGTCGA550.00393345420.45386920
AAACGCC550.00393345420.45386919
CAATAGT550.00393345420.4538694
ACGAGAC807.551254E-519.6868522
CTCGTAT706.975559E-419.28507839
TCGTATG706.975559E-419.28507840
TAGGACA706.975559E-419.2850784
AACGCAG8700.018.8786876
GTCTAAA600.006479725218.7622471
GAGTTAC600.006479725218.7622471
Produced by FastQC (version 0.11.2)