FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 30h_44.34100000005816.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 30h_44.34100000005816.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences443559
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC551.1252814E-732.72653641
TACCTAC300.005144457329.9993256
TATACTT300.005144457329.9993255
TATCTCG406.160939E-428.12436536
TATGCCG802.968507E-622.49949343
TCTCGTA802.968507E-622.49949338
CCCACGA855.0216986E-621.17599319
ACGGGAT654.2300034E-420.77110316
CGTCTAT550.003928164520.4586961
CGGGACA550.00393073120.4563918
CTCGTAT908.23411E-619.9995539
ACGAGAC1051.4417947E-619.2852822
CATCTCC18200.018.54353745
TACCCCG851.2025335E-418.52899420
GGGACTA1608.112693E-1018.28289818
GTCATAG1002.0384708E-518.0036531
GTGCAAG750.001107253518.0036531
GGGTTAA750.001107253518.0036531
ATCTCCG8000.017.99959445
TTGCATT901.8627409E-417.4996054
Produced by FastQC (version 0.11.2)