FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 30h_43.34100000005809.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 30h_43.34100000005809.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences307115
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGTGC207.025331E-444.9998369
CGCGCTC250.00210474735.9998718
TTCGTTA250.00210474735.9998727
CCGCGCT250.00210474735.9998717
TGCACCG250.00210474735.999875
CGTGCTC352.815657E-432.14274211
CGAACGT300.005141546529.9998914
CTCCGCG300.005141546529.99989115
CCCTTAA450.001223372325.0039791
AAGTACT1850.023.1080254
AAAAGTA1850.023.1080252
AAAGTAC1850.023.1080253
CTAGACC500.002260908522.499924
TACATGT500.002260908522.499924
GACGGTT500.002260908522.499928
GCCGTGC500.002260908522.4999212
TCTCCGA2900.022.49991845
ATCTCCG5650.022.30080245
ACGGGGC1459.094947E-1221.72405816
CGCTCGC550.00393015520.45447220
Produced by FastQC (version 0.11.2)