FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 30h_42.341000000057fb.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 30h_42.341000000057fb.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences461373
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACCG352.8184554E-432.1415375
GTATCAA7150.029.270881
GTATAGT507.140717E-527.0047471
GGTATCA2950.026.6996081
TAGCGTC602.4614725E-422.50395825
ATCTCCG6300.022.1443545
GTAGCGT654.2279123E-420.77288424
GCGTCGT654.2279123E-420.77288427
ACCGTGC550.003933954520.45370739
CGGGGGT1009.0343747E-720.2491717
TATCAAC10700.019.5552752
CGTCGTG706.966552E-419.28910428
ATCAACG11050.018.9358773
ATATCGG600.00650626518.74923110
TACACTG2400.018.7492315
AACGCAG11200.018.6822686
TCAACGC11250.018.599244
TTCTAAA851.2028359E-418.5286522
CAACGCA11350.018.4353685
TATACAG1102.250912E-618.4083375
Produced by FastQC (version 0.11.2)