FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 30h_41.341000000057ee.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 30h_41.341000000057ee.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences264346
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACGGGGTGGTATCCTGTCTCTTATACACATCTCCGA15280.5780303087620013No Hit
GGTATCAACGCAGAGTACGGGGTGGTATCCTGTCTCTTATACACATCTCCG14670.5549544914619475No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCCTGTCTCTTATACACATC13930.5269608770323743No Hit
GATACCACCCCGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCG12520.4736216927814304No Hit
GATACCACCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGA10910.41271666679276403No Hit
GCGTTGATACCACCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATC8650.3272226551564994No Hit
TTTTTGCGTTGATACCACCCCGTACTCTGCGTTGATACCTGTCTCTTATAC8030.3037685457695596No Hit
GTATCAACGCAGAGTACGGGGTGGTATCACTGTCTCTTATACACATCTCCG7710.2916631989892035No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAACTGTCTCTTATAC7210.2727485946448972No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCCTGTCTCTTATACACATCT6900.2610215399514273No Hit
GAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6800.257238619082566No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCTGTCTCTTATACACATCT6460.24437668812843774No Hit
GGTATCAACGCAGAGTACGGGGTGGTATCAACGCCTGTCTCTTATACACAT6350.24021547517269035No Hit
TTTTTGCGTTGATACCACCCCGTACTCTGCGTTGATACCCTGTCTCTTATA6290.2379457226513736No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA6180.2337845096956262No Hit
GTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6160.23302792552185392No Hit
GCGTTGATACCACCCCGTACTCTGCGTTGATACCCTGTCTCTTATACACAT6070.22962329673987877No Hit
GTATCAACGCAGAGTACGGGCAGTGGTATCTGTCTCTTATACACATCTCCG5880.2224357470890424No Hit
GTATCAACGCAGAGTACGGGCAGTGGTATCAACGCCTGTCTCTTATACACA5730.2167613657857505No Hit
TGATACCACCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCG5680.21486990535131986No Hit
GTATCAACGCAGAGTACGGGCAGTGGTATCCTGTCTCTTATACACATCTCC5670.21449161326443375No Hit
TATCAACGCAGAGTACGGGGTGGTATCCTGTCTCTTATACACATCTCCGAG5670.21449161326443375No Hit
GTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5580.21108698448245858No Hit
GTATCAACGCAGAGTACGGGTGGTATCAACGCCTGTCTCTTATACACATCT5370.20314285065784993No Hit
GTATCAACGCAGAGTACGGGCAGTGGTATCAACGCTGTCTCTTATACACAT5180.19595530100701355No Hit
GGTATCAACGCAGAGTACGGGCAGTGGTATCTGTCTCTTATACACATCTCC5170.1955770089201274No Hit
CGTTGATACCACCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCT5170.1955770089201274No Hit
ATACCACTGCCCGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCC4890.18498483048731587No Hit
GGTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAACTGTCTCTTATA4810.18195849379222684No Hit
TTTTTGCGTTGATACCACTGCCCGTACTCTGCGTTGATACCTGTCTCTTAT4740.17931044918402397No Hit
GATACCACTGCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCC4550.17212289953318757No Hit
TTTTTTGCGTTGATACCACCCCGTACTCTGCGTTGATACCTGTCTCTTATA4520.17098802327252918No Hit
TTTTTGCGTTGATACCACCCCGTACTCTGCGTTGATACTGTCTCTTATACA4510.17060973118564307No Hit
ATCAACGCAGAGTACGGGGTGGTATCAACGCCTGTCTCTTATACACATCTC4510.17060973118564307No Hit
GGTATCAACGCAGAGTACGGGGTGGTATCACTGTCTCTTATACACATCTCC4160.15736950814462863No Hit
GCGTTGATACCACCCCGTACTCTGCGTTGATCTGTCTCTTATACACATCTC4130.15623463188397024No Hit
GGTATCAACGCAGAGTACGGGCAGTGGTATCCTGTCTCTTATACACATCTC4050.15320829518888124No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4040.15283000310199513No Hit
ATACCACTGCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCG4000.1513168347544506No Hit
GGTATCAACGCAGAGTACGGGGTGGTATCTGTCTCTTATACACATCTCCGA3970.15018195849379223No Hit
TGATACCACCCCGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCC3890.14715562179870323No Hit
GCGTTGATACCACCCCGTACTCTGCGTTGATACTGTCTCTTATACACATCT3830.14488586927738645No Hit
GCGTTGATACCACTGCCCGTACTCTGCGTTGATACCTGTCTCTTATACACA3780.14299440884295583No Hit
CGTTGATACCACTGCCCGTACTCTGCGTTGATACCTGTCTCTTATACACAT3740.1414812404954113No Hit
GGTATCAACGCAGAGTACGGGGTGGTATCAACGCTGTCTCTTATACACATC3650.1380766117134362No Hit
GATACCACCCCGTACTCTGCGTTGATACTGTCTCTTATACACATCTCCGAG3610.13656344336589166No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCTGTCTCTTATACACATCTC3570.13505027501834718No Hit
GATACCACTGCCCGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTC3560.13467198293146104No Hit
GCGTTGATACCACCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCT3530.13353710667080265No Hit
GTATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAACTGTCTCTTAT3510.13278052249703043No Hit
ATACCACCCCGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGA3440.13013247788882754No Hit
GGTATCAACGCAGAGTACGGGCAGTGGTATCAACGCCTGTCTCTTATACAC3420.12937589371505526No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAACTGTCTCTTATACA3420.12937589371505526No Hit
CGTTGATACCACCCCGTACTCTGCGTTGATACCCTGTCTCTTATACACATC3410.12899760162816915No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA3360.1271061411937385No Hit
TATCAACGCAGAGTACGGGCAGTGGTATCAACGCCTGTCTCTTATACACAT3170.1199185915429021No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAACTGTCTCTTATA3140.11878371528224371No Hit
GTATCAACGCAGAGTACGGGCAGTGGTATCAACCTGTCTCTTATACACATC3120.11802713110847148No Hit
TATCAACGCAGAGTACGGGTGGTATCAACGCCTGTCTCTTATACACATCTC3050.11537908650026858No Hit
TATCAACGCAGAGTACGGGGTGGTATCACTGTCTCTTATACACATCTCCGA3020.1142442102396102No Hit
CGTTGATACCACTGCCCGTACTCTGCGTTGATACCCTGTCTCTTATACACA3010.1138659181527241No Hit
TATCAACGCAGAGTACGGGCAGTGGTATCAACGCTGTCTCTTATACACATC2990.11310933397895183No Hit
GCGTTGATACCACTGCCCGTACTCTGCGTTGATACCCTGTCTCTTATACAC2950.11159616563140731No Hit
GTTGATACCACTGCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATC2930.11083958145763506No Hit
GAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2890.10932641311009056No Hit
GATACCACCCGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGA2860.10819153684943218No Hit
GTATCAACGCAGAGTACGGGGTATCAACGCCTGTCTCTTATACACATCTCC2830.1070566605887738No Hit
GGTATCAACGCAGAGTACGGGCAGTGGTATCAACGCTGTCTCTTATACACA2820.1066783685018877No Hit
GGTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA2800.10592178432811541No Hit
TTTTTTGCGTTGATACCACCCCGTACTCTGCGTTGATACCCTGTCTCTTAT2650.10024740302482353No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCCGT302.1592623E-644.99962244
TATGCCG356.230406E-638.57110643
GTATGCC356.230406E-638.57110642
TGCCGTC356.230406E-638.57110645
CACTATA504.8388756E-836.013321
GTAAATA250.002100361336.013321
CACCGAC250.002104267235.999712
CGTATGC401.556131E-533.74971841
ATATGTA352.808435E-432.1547551
TTGGAGA352.8146803E-432.142594
ACCGACT352.8146803E-432.1425913
ATGGTAT352.8146803E-432.1425921
CACTCTA300.00513087830.0111031
ACTATAG602.4072506E-729.9997462
CTCGTAT453.4809873E-529.99974639
AGATATA300.00514038429.99974629
GGTCCGG300.00514038429.9997469
AGGTTTG300.00514038429.99974629
ATATAAA300.00514038429.99974631
AATCTCG300.00514038429.99974636
Produced by FastQC (version 0.11.2)