FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 30h_39.341000000057c4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 30h_39.341000000057c4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences487386
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTCTACTTATCTCGTATGCCGTC9870.20250889438761066No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG1650.031.36284843
TATCTCG1650.031.36284836
GGTATCA4350.030.0115641
CCACGAG2000.028.12429420
CGTATGC1850.027.97227141
CTCGTAT2100.026.78504239
CACGAGA2100.026.78504221
TCGTATG2100.025.7136440
CTACTTA1850.025.539931
TCTCGTA2250.024.99937438
CGTAATG450.0012257224.99937414
ACGAGAC2350.024.89299222
TACTTAT1900.024.86779832
ACTTATC1900.024.86779833
GTATCAA10300.024.4754491
CCCACGA2300.024.45590819
CTTATCT1850.024.32371334
TCTACTT2000.023.62683130
TTATCTC1950.023.07634435
ATGCCGT2250.022.99942444
Produced by FastQC (version 0.11.2)