FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 30h_38.341000000057b7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 30h_38.341000000057b7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences418695
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTCTACTTATCTCGTATGCCGTC5970.14258589187833626No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG1250.034.19981843
TATCTCG1300.031.1536836
TGCCGTC1400.030.5355545
TCTCGTA1450.029.48260338
CTCGTAT1550.027.580539
TCGTATG1550.027.580540
ACTTATC1400.027.32128133
CGTATGC1600.026.71860941
ACGAGAC1600.026.71860922
CCACGAG1700.026.47044820
ATGCCGT1650.025.90895344
GCCCACG1700.025.14692518
TTAGGCG450.001225290224.99986630
TGGACCG450.001225290224.9998665
CTACTTA1550.024.67728831
TACTTAT1550.024.67728832
CGAGACC1550.024.67728823
CCCACGA1750.024.42844219
GGTATCA6350.023.7457051
CTTATCT1650.023.18169634
Produced by FastQC (version 0.11.2)