FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 30h_37.341000000057ab.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 30h_37.341000000057ab.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences587930
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTCTACTTATCTCGTATGCCGTC7110.12093276410457027No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC1350.033.34013745
TATGCCG1400.032.14394843
GGACCGT300.005142241730.0035726
ATCGATA300.00514652129.9984723
CCCACGA1550.029.03077719
ACGAGAC1600.028.12356422
CTCGTAT1650.027.27133639
CACGAGA1650.027.27133621
CCACGAG1650.027.27133620
GCCCACG1800.026.24866118
ATGACCG450.001224990925.002985
TCGTATG1800.024.99872640
CTACTTA1301.8189894E-1224.22953431
TACTTAT1301.8189894E-1224.22953432
ACTTATC1353.6379788E-1223.33214433
ATGCCGT2050.023.04956644
CGCGCAA500.00226397522.49885220
TGCGCGT500.00226397522.49885227
TCTCGTA2000.022.49885238
TAATACT855.027532E-621.175394
Produced by FastQC (version 0.11.2)