FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 30h_36.3410000000579e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 30h_36.3410000000579e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences506877
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTCTACTTATCTCGTATGCCGTC5830.11501804185236261No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAAGG403.452824E-739.375432
TATGCCG1350.031.6638943
TCGTATG1450.029.48017340
TACTTAT1250.028.79747432
CGGGGGA406.157401E-428.12808217
TATCTCG1500.026.99763336
CGTATGC1750.025.71202941
TTATCTC1450.024.82540935
CACGAGA1750.024.42883921
CTCGTAT1750.024.42642839
ACTTATC1400.024.1050333
ACGAGAC1750.023.1431122
GCCCACG1850.023.11064118
CCACGAG1850.023.1083620
GTATATA802.9639468E-622.5046861
TGCCGTC1800.022.50024845
ACGCCTA500.002262732722.50024621
CTACTTA1500.022.49802831
TCTCGTA1900.022.49802838
ATGCCGT1800.022.49802644
Produced by FastQC (version 0.11.2)