FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 30h_35.34100000005781.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 30h_35.34100000005781.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences404043
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTCTACTTATCTCGTATGCCGTC6340.15691399182760252No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCCCGA250.002105580435.99970216
TGCCGTC751.6971171E-929.99975245
GCTTACC507.1294606E-527.0098061
TATGCCG957.385097E-1026.05241643
ACAGCGC450.001225236624.9997948
CTCGTAT1001.3387762E-924.74979639
CACGAGA1052.3555913E-923.57123421
GGCCGAC500.002262196522.4998157
TTATCGT702.6975635E-522.4998134
AGTGATA702.6975635E-522.4998138
TCGTATG1104.0326995E-922.49981340
AAAAAGT1551.8189894E-1221.78211
CGTCTAT754.580312E-521.0076271
TATCGTT654.231914E-420.7690585
ACGAGAC1201.0999429E-820.6248322
ACTGATA550.003932376420.4543768
TCTAGGG550.003932376420.4543763
ACGGGTA550.003932376420.4543765
CGGGGGT550.003932376420.45437617
TATCTCG1109.944779E-820.45437636
Produced by FastQC (version 0.11.2)