FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 30h_34.34100000005774.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 30h_34.34100000005774.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences383376
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTCTACTTATCTCGTATGCCGTC5030.1312027878636117No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC751.8189894E-1236.0042845
GTCTAGG458.6540604E-735.0132941
GTATAGG352.8106227E-432.1550671
GCCTCGA352.8170858E-432.14248311
CGGTTTT300.00514332229.99965320
CCGTCCA300.00514332229.9996539
TATGCCG952.3646862E-1128.42072343
CGTGCAA406.159005E-428.1246749
ATGCCGT1004.5474735E-1126.99968744
ACGAGAC1058.367351E-1125.7139922
ACGGGTC450.001225146524.99970816
GTATTGG702.689023E-522.5085471
CAATAGG500.00225689122.5085471
ACCGTGC602.4635432E-422.499747
TACTTAT1104.0326995E-922.49973932
CCGTGCA500.002262030722.4997398
CAATTAC754.5789388E-521.0079771
CTACTCA754.5927736E-520.9997584
TATCTCG1201.0995791E-820.62476236
GCTACAC550.00393209220.4543083
Produced by FastQC (version 0.11.2)