FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 30h_33.34100000005767.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 30h_33.34100000005767.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences446441
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATACCACCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGA14940.33464668343633314No Hit
GATACCACCCCGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCG12470.2793202237249715No Hit
GTATCAACGCAGAGTACGGGGTGGTATCCTGTCTCTTATACACATCTCCGA12140.27192842951252233No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCCTGTCTCTTATACACATC10970.24572115912293002No Hit
GCGTTGATACCACCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATC10830.2425852464267395No Hit
GGTATCAACGCAGAGTACGGGGTGGTATCCTGTCTCTTATACACATCTCCG10090.22600970788973235No Hit
TGATACCACCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCG9330.208986181824698No Hit
GTATCAACGCAGAGTACGGGGTGGTATCACTGTCTCTTATACACATCTCCG8980.20114640008422166No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAACTGTCTCTTATAC7770.17404315463857487No Hit
TTTTTGCGTTGATACCACCCCGTACTCTGCGTTGATACCTGTCTCTTATAC7500.1679953230102074No Hit
CGTTGATACCACCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCT6430.14402799026075114No Hit
GCGTTGATACCACCCCGTACTCTGCGTTGATACTGTCTCTTATACACATCT6070.13596421475626116No Hit
GATACCACCCCGTACTCTGCGTTGATACTGTCTCTTATACACATCTCCGAG5910.1323803145320434No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCTGTCTCTTATACACATCT5900.1321563207680298No Hit
TGATACCACCCCGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCC5900.1321563207680298No Hit
GGTATCAACGCAGAGTACGGGGTGGTATCAACGCCTGTCTCTTATACACAT5880.1317083332400026No Hit
GCGTTGATACCACCCCGTACTCTGCGTTGATACCCTGTCTCTTATACACAT5830.13058836441993454No Hit
GGTATCAACGCAGAGTACGGGGTGGTATCACTGTCTCTTATACACATCTCC5540.12409254526353987No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCTACTTATCTCGTATGCCGTC5490.12297257644347181No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCCTGTCTCTTATACACATCT5440.12185260762340377No Hit
TATCAACGCAGAGTACGGGGTGGTATCCTGTCTCTTATACACATCTCCGAG5280.11826870739918602No Hit
GTATCAACGCAGAGTACGGGACTAATCCTAGCCCTAGCCCTACACCTGTCT4910.10998093813068244No Hit
GGCTAGGATTAGTCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATC4760.1066210316704783No Hit
ATACCACTGCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCG4740.10617304414245107No Hit
GGTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAACTGTCTCTTATA4650.10415710026632859No Hit
GGGCTAGGGCTAGGATTAGTCCCGTACTCTGCGTTGATACCTGTCTCTTAT4650.10415710026632859No Hit
TTTTTGCGTTGATACCACCCCGTACTCTGCGTTGATACCCTGTCTCTTATA4530.10146917509816526No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAACTGTCTCTTATACA4530.10146917509816526No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGACGC207.0300256E-444.99820719
TTCGACG207.0300256E-444.99820718
GCCCCGC250.002106142735.99856638
CTTCGAC250.002106142735.99856617
ACGCCTA250.002106142735.99856622
CGACGCC250.002106142735.99856620
CGCCTAC250.002106142735.99856623
ATTGCTT250.002106142735.99856632
TGCCGTC458.686784E-734.99860845
AAGCGTC401.5564732E-533.75621411
ATAAGTA401.5588628E-533.7486572
CTTAGTA352.8182898E-432.141582
ATGCCGT501.9786457E-631.49874544
GTGTAGG3350.030.9012171
TATAAGT453.4817298E-530.0055261
AATTAGT300.00514211130.0021656
TATCAGT453.48706E-529.99880622
TTAAGGG300.005144928629.9988043
CGTGTAA300.005144928629.99880444
TAAGGGA300.005144928629.9988044
Produced by FastQC (version 0.11.2)