FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 30h_32.3410000000575b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 30h_32.3410000000575b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1065
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGATATAAATCTCGTATGCCGTC34732.582159624413144No Hit
CTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGTC434.037558685446009No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGTC282.629107981220657No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGTC232.15962441314554No Hit
CTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGTC222.0657276995305165No Hit
CTTATACACATCTCCGAGCCCACGAGACGAATTGCTATCTCGTATGCCGTC191.784037558685446RNA PCR Primer, Index 27 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGTC171.5962441314553992No Hit
CTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGTC111.0328638497652582No Hit
TTATACACATCTCCGAGCCCACGAGACAGATATAAATCTCGTATGCCGTCT80.7511737089201878No Hit
CTTATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGTC70.6572769953051643TruSeq Adapter, Index 12 (95% over 21bp)
TTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGTCT60.5633802816901409No Hit
CTTATACACATCTCCGAGCCCACGAGACCATTCCGAATCTCGTATGCCGTC50.4694835680751174RNA PCR Primer, Index 46 (95% over 21bp)
CTTATACACATCTCCGAGCCCCACGAGACAGATATAAATCTCGTATGCCGT40.3755868544600939No Hit
TTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGTCT30.28169014084507044RNA PCR Primer, Index 44 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACCAAGGCGAATCTCGTATGCCGTC30.28169014084507044No Hit
CTTATACACATCTCCGAGCCCACGAGACAGATATAAATCTCGTATGCCGCC30.28169014084507044No Hit
CTTATACACATCTCCGAGCCCACGAGACAGATATAAATCTCGTCTGCCGTC20.18779342723004694No Hit
CTTATACACATCTCCGAGCCCACGAGACCTATTCAAATCTCGTATGCCGTC20.18779342723004694RNA PCR Primer, Index 13 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACAGATATAAATATCGTATGCCGTC20.18779342723004694No Hit
CTTATACACATCTCCGAGCCCACGAGACCGATATAAATCTCGTATGCCGTC20.18779342723004694No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20.18779342723004694No Hit
TTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGTCT20.18779342723004694Illumina PCR Primer Index 8 (95% over 21bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20.18779342723004694No Hit
TTATACACATCTCCGAGCCCACGAGACGAATTGCTATCTCGTATGCCGTCT20.18779342723004694RNA PCR Primer, Index 38 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACAGATATAAATCGTATGCCGTCTT20.18779342723004694No Hit
CTTATACACACTCCGAGCCCACGAGACAGATATAAATCTCGTATGCCGTCT20.18779342723004694No Hit
CTTCAAGCCTTGACGCTTACACATGATTCCAGTTACATGCGCCATTAGCCT20.18779342723004694No Hit
CTTATACACATCTCCGAGCCCACGAGACAGATCTAAATCTCGTATGCCGTC20.18779342723004694No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCTCG352.7734131E-845.00000436
ACAGATA352.7734131E-845.00000427
TAAATCT352.7734131E-845.00000434
AAATCTC352.7734131E-845.00000435
GATATAA352.7734131E-845.00000430
GACAGAT352.7734131E-845.00000426
TATAAAT352.7734131E-845.00000432
ATAAATC352.7734131E-845.00000433
CAGATAT352.7734131E-845.00000428
AGACAGA352.7734131E-845.00000425
AGATATA352.7734131E-845.00000429
GAGACAG352.7734131E-845.00000424
ATATAAA352.7734131E-845.00000431
AGCCCAC550.045.017
GCCCACG550.045.018
TGCCGTC453.274181E-1145.045
CCACGAG550.045.020
GAGCCCA550.045.016
CACGAGA550.045.021
CCCACGA550.045.019
Produced by FastQC (version 0.11.2)