FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 30h_31.3410000000574e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 30h_31.3410000000574e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1471
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTCTTAGTATCTCGTATGCCGTC16211.012916383412644No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGTC493.331067301155676No Hit
CTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGTC322.175390890550646No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGTC271.8354860639021073No Hit
CTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGTC261.7675050985723997No Hit
CTTATACACATCTCCGAGCCCACGAGACGATCGTGTATCTCGTATGCCGTC181.2236573759347382No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGTC110.7477906186267845No Hit
CTTATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGTC110.7477906186267845TruSeq Adapter, Index 12 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACAGTGTCTTATCTCGTATGCCGTC80.5438477226376615No Hit
CTTATACACATCTCCGAGCCCACGAGACCATTCCGAATCTCGTATGCCGTC80.5438477226376615RNA PCR Primer, Index 46 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACGAATTGCTATCTCGTATGCCGTC60.4078857919782461RNA PCR Primer, Index 27 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACCTTGCAGAATCTCGTATGCCGTC50.3399048266485384No Hit
TCTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGT40.27192386131883073No Hit
CTTATACACATCTCCGAGCCCACGAGACTCAACTGTATCTCGTATGCCGTC40.27192386131883073No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA30.20394289598912305No Hit
CTTATACACATCTCCGAGCCCACGAGACCTATTCAAATCTCGTATGCCGTC20.13596193065941536RNA PCR Primer, Index 13 (95% over 21bp)
TTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGTCT20.13596193065941536Illumina PCR Primer Index 8 (95% over 21bp)
TTGCGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTGCGAAGCAGT20.13596193065941536No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCTACTTATCTCGTATGCCGTC20.13596193065941536No Hit
GCGTTGATACCACTGCTTCGCAAAAAAAAAAAAGTACTCTGCGTTGATACC20.13596193065941536No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20.13596193065941536No Hit
TTATACACATCTCCGAGCCCACGAGACGAATTGCTATCTCGTATGCCGTCT20.13596193065941536RNA PCR Primer, Index 38 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTCCG600.045.00000410
TCTCCGA600.045.00000411
AATCTCG204.950902E-445.036
AGCCCAC650.041.5384617
GTATGCC650.041.5384642
ATCTCGT650.041.5384637
CACATCT650.041.538467
GCCCACG650.041.5384618
TGCCGTC650.041.5384645
CCACGAG650.041.5384620
TATGCCG650.041.5384643
CATCTCC650.041.538469
GAGCCCA650.041.5384616
CACGAGA650.041.5384621
CCCACGA650.041.5384619
ACATCTC650.041.538468
ACACATC650.041.538466
TCTCGTA650.041.5384638
ATGCCGT650.041.5384644
CGTATGC650.041.5384641
Produced by FastQC (version 0.11.2)