FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 30h_30.34100000005731.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 30h_30.34100000005731.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences472187
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG751.6989361E-929.99816540
CGAGACG453.48799E-529.99816523
CGTATGC856.4646883E-926.4689741
TATGCCG856.4646883E-926.4689743
GTATCAA7100.023.7787421
ACGAGAC1052.3592293E-923.56998622
CCGAGCC1056.085793E-821.42953114
GGTATCA3600.021.2599561
CGAGCCC1109.948235E-820.45546215
CTCGTAT1109.959331E-820.45329539
CACGAGA1109.959331E-820.45329521
TGCCGTC1251.7618731E-819.79878845
GGACTAG706.973032E-419.2865776
ATCTCCG7000.019.28453445
GCCGCTC600.006503266318.750848
AACTGCG600.006503266318.750847
ATTAGAG600.006507244418.7488543
TGACGTC600.006507244418.74885423
CATCTCC16800.018.61493345
TCCGAGC1354.2540705E-818.33415213
Produced by FastQC (version 0.11.2)