FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 30h_3.34100000005526.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 30h_3.34100000005526.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences514773
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAGGG453.4890414E-529.9976042
GTATCAA4500.021.5024591
GGTATCA2550.021.1788941
GTACTAA654.2305826E-420.7716061
CGGTACT908.2354945E-620.00034530
GCGGTAC951.3133309E-518.94769729
TCGTACA1354.2577085E-818.33364925
GGTACTA1053.1091593E-517.14148731
TTCGTAC1459.635005E-817.0692624
CGTACAT1459.635005E-817.0692626
AAATTCG1459.645555E-817.067621
TGCGGTA1205.1610787E-616.87529228
CCTATAG1104.624985E-516.3655091
GTGTTAA1257.6015003E-616.2018551
AATTCGT1552.0614061E-715.96646822
TATATGA850.002563951315.8810842
TCCTACA850.002563951315.8810842
TACTAAG850.002563951315.8810842
CTTAGAC850.002563951315.8810843
GTATAGG1004.157846E-415.7518021
Produced by FastQC (version 0.11.2)