##FastQC	0.11.2
>>Basic Statistics	pass
#Measure	Value
Filename	H23NVBCXX l02n01 30h_29.34100000005724.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1583
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.68288060644346	38.0	32.0	38.0	27.0	38.0
2	34.59317751105496	38.0	32.0	38.0	32.0	38.0
3	34.4801010739103	38.0	32.0	38.0	32.0	38.0
4	34.80290587492104	38.0	32.0	38.0	32.0	38.0
5	34.88186986734049	38.0	32.0	38.0	32.0	38.0
6	36.526847757422615	38.0	38.0	40.0	32.0	40.0
7	35.933670246367654	38.0	38.0	40.0	32.0	40.0
8	36.10423246999368	38.0	38.0	40.0	32.0	40.0
9	36.16298168035376	38.0	38.0	40.0	32.0	40.0
10	36.009475679090336	38.0	38.0	40.0	27.0	40.0
11	36.02210991787745	38.0	38.0	40.0	32.0	40.0
12	35.70878079595705	38.0	38.0	40.0	27.0	40.0
13	35.67845862286797	38.0	38.0	40.0	27.0	40.0
14	35.7037271004422	38.0	38.0	40.0	27.0	40.0
15	35.667719519898924	38.0	38.0	40.0	27.0	40.0
16	35.416298168035375	38.0	32.0	40.0	27.0	40.0
17	35.34112444725206	38.0	32.0	40.0	27.0	40.0
18	35.308907138344914	38.0	32.0	40.0	27.0	40.0
19	35.52621604548326	38.0	32.0	40.0	27.0	40.0
20	35.4857864813645	38.0	32.0	40.0	27.0	40.0
21	35.742261528742894	38.0	38.0	40.0	27.0	40.0
22	35.85344283006949	38.0	38.0	40.0	27.0	40.0
23	35.83006948831333	38.0	38.0	40.0	27.0	40.0
24	35.60833859759949	38.0	38.0	40.0	27.0	40.0
25	35.2008843967151	38.0	32.0	40.0	27.0	40.0
26	35.04485154769425	38.0	32.0	40.0	27.0	40.0
27	35.1282375236892	38.0	32.0	40.0	27.0	40.0
28	35.60328490208465	38.0	32.0	40.0	27.0	40.0
29	35.202779532533164	38.0	32.0	40.0	27.0	40.0
30	35.29564118761844	38.0	32.0	40.0	27.0	40.0
31	35.371446620341125	38.0	32.0	40.0	27.0	40.0
32	35.389134554643086	38.0	32.0	40.0	27.0	40.0
33	35.353758686039164	38.0	32.0	40.0	27.0	40.0
34	35.55653821857233	38.0	32.0	40.0	27.0	40.0
35	35.25078963992419	38.0	32.0	40.0	27.0	40.0
36	35.13644977890082	38.0	32.0	40.0	27.0	40.0
37	35.005053695514846	38.0	32.0	40.0	27.0	40.0
38	35.25457991156033	38.0	32.0	40.0	27.0	40.0
39	35.049905243209096	38.0	32.0	40.0	27.0	40.0
40	35.23752368919772	38.0	32.0	40.0	27.0	40.0
41	35.19898926089703	38.0	32.0	40.0	27.0	40.0
42	35.18888186986734	38.0	32.0	40.0	27.0	40.0
43	35.293746051800376	38.0	32.0	40.0	27.0	40.0
44	34.93556538218572	38.0	32.0	40.0	27.0	40.0
45	35.17182564750474	38.0	32.0	40.0	27.0	40.0
46	34.70688566013898	38.0	32.0	40.0	27.0	40.0
47	34.70688566013898	38.0	32.0	38.0	27.0	40.0
48	34.837650031585596	38.0	32.0	40.0	27.0	40.0
49	34.80732785849653	38.0	32.0	40.0	27.0	40.0
50	34.691092861655086	38.0	32.0	38.0	27.0	40.0
51	33.84712571067593	38.0	32.0	38.0	13.0	40.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	5.30952380952381
1101	2	0.679894179894184
1101	3	-1.845238095238095
1101	4	0.2037037037037095
1101	5	0.646825396825399
1101	6	-0.896825396825399
1101	7	-13.33862433862435
1101	8	-12.585978835978842
1101	9	-13.02248677248678
1101	10	-9.392857142857146
1101	11	-3.202380952380949
1101	12	0.011904761904759198
1101	13	-9.710317460317462
1101	14	-9.514550264550259
1101	15	0.588624338624335
1101	16	-9.689153439153444
1101	17	-8.06878306878307
1101	18	-0.46031746031746223
1101	19	2.8002645502645507
1101	20	1.9193121693121569
1101	21	0.5238095238095255
1101	22	0.5304232804232853
1101	23	-2.429894179894184
1101	24	-10.617724867724867
1101	25	-1.654761904761905
1101	26	-8.746031746031747
1101	27	-2.002645502645507
1101	28	-1.8703703703703738
1101	29	0.7037037037037095
1101	30	1.3769841269841265
1101	31	0.9960317460317469
1101	32	4.199735449735442
1101	33	4.527777777777779
1101	34	-9.693121693121697
1101	35	-9.612433862433868
1101	36	-1.578042328042315
1101	37	-9.415343915343918
1101	38	-2.2632275132275197
1101	39	-1.5687830687830697
1101	40	-2.0092592592592524
1101	41	-9.5026455026455
1101	42	-8.93386243386243
1101	43	2.904761904761905
1101	44	-8.271164021164019
1101	45	-9.80291005291005
1101	46	-9.25925925925926
1101	47	-1.216931216931222
1101	48	-7.531746031746032
1101	49	-1.4616402116402085
1101	50	-2.198412698412696
1101	51	-1.4669312169312079
2201	1	5.30952380952381
2201	2	-4.653439153439148
2201	3	-6.178571428571427
2201	4	-0.4629629629629548
2201	5	3.646825396825399
2201	6	-4.896825396825399
2201	7	-0.8386243386243493
2201	8	1.5806878306878218
2201	9	3.8108465608465565
2201	10	-3.892857142857146
2201	11	-0.0357142857142847
2201	12	0.6785714285714235
2201	13	4.1230158730158735
2201	14	1.6521164021164054
2201	15	-0.41137566137566495
2201	16	1.4775132275132208
2201	17	4.764550264550266
2201	18	3.8730158730158735
2201	19	2.8002645502645507
2201	20	2.585978835978821
2201	21	1.8571428571428612
2201	22	3.863756613756621
2201	23	1.7367724867724803
2201	24	2.5489417989417973
2201	25	1.845238095238095
2201	26	-4.579365079365079
2201	27	2.1640211640211575
2201	28	3.2962962962962905
2201	29	2.370370370370374
2201	30	1.3769841269841265
2201	31	3.6626984126984112
2201	32	3.8664021164021065
2201	33	-3.4722222222222214
2201	34	1.4735449735449677
2201	35	1.5542328042327966
2201	36	-0.07804232804231503
2201	37	3.7513227513227463
2201	38	3.2367724867724803
2201	39	5.264550264550266
2201	40	4.157407407407412
2201	41	4.330687830687836
2201	42	4.899470899470906
2201	43	-4.761904761904763
2201	44	1.2288359788359813
2201	45	4.030423280423285
2201	46	4.574074074074076
2201	47	4.616402116402114
2201	48	4.634920634920633
2201	49	3.705026455026456
2201	50	-3.698412698412696
2201	51	1.0330687830687921
2202	1	-0.6904761904761898
2202	2	-0.320105820105816
2202	3	4.154761904761905
2202	4	3.2037037037037095
2202	5	1.646825396825399
2202	6	2.103174603174601
2202	7	2.1613756613756507
2202	8	2.9140211640211575
2202	9	3.8108465608465565
2202	10	3.107142857142854
2202	11	2.9642857142857153
2202	12	3.6785714285714235
2202	13	2.7896825396825378
2202	14	-7.347883597883591
2202	15	0.588624338624335
2202	16	0.8108465608465565
2202	17	0.4312169312169303
2202	18	-5.793650793650794
2202	19	-2.1997354497354493
2202	20	0.5859788359788212
2202	21	2.5238095238095255
2202	22	2.5304232804232853
2202	23	1.070105820105816
2202	24	-0.11772486772486701
2202	25	-1.8214285714285694
2202	26	0.08730158730158877
2202	27	-0.5026455026455068
2202	28	2.629629629629626
2202	29	-3.9629629629629584
2202	30	-13.289682539682541
2202	31	-9.003968253968253
2202	32	-5.13359788359789
2202	33	-0.47222222222222143
2202	34	0.8068783068783034
2202	35	1.5542328042327966
2202	36	0.921957671957685
2202	37	1.084656084656082
2202	38	3.2367724867724803
2202	39	3.9312169312169303
2202	40	1.4907407407407476
2202	41	0.9973544973545003
2202	42	4.232804232804234
2202	43	2.904761904761905
2202	44	-5.437830687830687
2202	45	-4.6362433862433825
2202	46	-5.092592592592592
2202	47	-2.3835978835978864
2202	48	3.9682539682539684
2202	49	1.0383597883597915
2202	50	3.301587301587304
2202	51	2.033068783068792
1102	1	3.30952380952381
1102	2	1.679894179894184
1102	3	2.154761904761905
1102	4	3.2037037037037095
1102	5	-0.35317460317460103
1102	6	0.7698412698412653
1102	7	2.828042328042315
1102	8	3.580687830687822
1102	9	3.144179894179885
1102	10	1.7738095238095184
1102	11	0.9642857142857153
1102	12	3.6785714285714235
1102	13	3.456349206349202
1102	14	3.6521164021164054
1102	15	3.2552910052909994
1102	16	1.4775132275132208
1102	17	4.097883597883595
1102	18	3.206349206349202
1102	19	2.1335978835978864
1102	20	3.2526455026454926
1102	21	1.8571428571428612
1102	22	3.863756613756621
1102	23	3.7367724867724803
1102	24	3.2156084656084687
1102	25	3.845238095238095
1102	26	4.420634920634917
1102	27	4.830687830687829
1102	28	2.629629629629626
1102	29	-1.9629629629629548
1102	30	4.043650793650791
1102	31	3.6626984126984112
1102	32	1.8664021164021065
1102	33	4.861111111111114
1102	34	4.140211640211639
1102	35	4.220899470899468
1102	36	3.5886243386243493
1102	37	1.7513227513227463
1102	38	3.2367724867724803
1102	39	4.597883597883595
1102	40	4.157407407407412
1102	41	3.6640211640211646
1102	42	4.232804232804234
1102	43	4.238095238095241
1102	44	5.562169312169317
1102	45	1.363756613756614
1102	46	-0.09259259259259522
1102	47	3.949735449735442
1102	48	2.6349206349206327
1102	49	-0.2949735449735442
1102	50	1.9682539682539684
1102	51	2.033068783068792
2203	1	-0.6904761904761898
2203	2	-0.820105820105816
2203	3	1.154761904761905
2203	4	0.2037037037037095
2203	5	0.646825396825399
2203	6	-0.896825396825399
2203	7	-0.33862433862434926
2203	8	1.9140211640211575
2203	9	2.477513227513221
2203	10	3.107142857142854
2203	11	2.297619047619051
2203	12	3.011904761904759
2203	13	1.7896825396825378
2203	14	3.485449735449741
2203	15	2.588624338624335
2203	16	3.3108465608465565
2203	17	-2.5687830687830697
2203	18	2.5396825396825378
2203	19	-0.19973544973544932
2203	20	1.9193121693121569
2203	21	-3.7261904761904745
2203	22	-3.7195767195767147
2203	23	-1.179894179894184
2203	24	-0.617724867724867
2203	25	-1.904761904761905
2203	26	0.7539682539682531
2203	27	0.4973544973544932
2203	28	1.1296296296296262
2203	29	2.2037037037037095
2203	30	0.37698412698412653
2203	31	0.7460317460317469
2203	32	-3.050264550264558
2203	33	0.5277777777777786
2203	34	-1.1931216931216966
2203	35	-2.6124338624338677
2203	36	-6.328042328042315
2203	37	0.334656084656082
2203	38	3.2367724867724803
2203	39	3.9312169312169303
2203	40	3.4907407407407476
2203	41	-3.2526455026454997
2203	42	2.56613756613757
2203	43	1.904761904761905
2203	44	4.728835978835981
2203	45	1.6970899470899496
2203	46	2.2407407407407405
2203	47	1.783068783068778
2203	48	2.4682539682539684
2203	49	3.0383597883597915
2203	50	3.301587301587304
2203	51	1.0330687830687921
1103	1	3.30952380952381
1103	2	-0.320105820105816
1103	3	-4.178571428571427
1103	4	-0.7962962962962905
1103	5	-0.35317460317460103
1103	6	2.7698412698412653
1103	7	3.4947089947089864
1103	8	4.9140211640211575
1103	9	1.144179894179885
1103	10	-4.559523809523814
1103	11	-5.369047619047617
1103	12	0.011904761904759198
1103	13	1.456349206349202
1103	14	3.6521164021164054
1103	15	1.9219576719576708
1103	16	-4.855820105820111
1103	17	0.4312169312169303
1103	18	-2.4603174603174622
1103	19	-4.866402116402117
1103	20	-5.747354497354511
1103	21	-0.4761904761904745
1103	22	-0.46957671957671465
1103	23	-5.263227513227516
1103	24	2.5489417989417973
1103	25	0.8452380952380949
1103	26	-3.912698412698415
1103	27	0.4973544973544932
1103	28	-0.3703703703703738
1103	29	-3.9629629629629584
1103	30	-4.289682539682541
1103	31	-2.003968253968253
1103	32	-4.466931216931226
1103	33	-6.138888888888889
1103	34	2.8068783068783034
1103	35	-5.4457671957672
1103	36	-0.07804232804231503
1103	37	-4.582010582010586
1103	38	0.2367724867724803
1103	39	-3.0687830687830697
1103	40	0.4907407407407476
1103	41	-4.0026455026455
1103	42	3.56613756613757
1103	43	0.5714285714285694
1103	44	1.2288359788359813
1103	45	-4.30291005291005
1103	46	-4.425925925925927
1103	47	-6.38359788359789
1103	48	-4.365079365079364
1103	49	-4.628306878306876
1103	50	0.30158730158730407
1103	51	2.033068783068792
2204	1	2.30952380952381
2204	2	3.679894179894184
2204	3	4.154761904761905
2204	4	3.2037037037037095
2204	5	3.646825396825399
2204	6	3.103174603174601
2204	7	3.1613756613756507
2204	8	0.9140211640211575
2204	9	3.477513227513221
2204	10	1.107142857142854
2204	11	3.297619047619051
2204	12	4.011904761904759
2204	13	2.7896825396825378
2204	14	2.985449735449741
2204	15	3.588624338624335
2204	16	4.8108465608465565
2204	17	5.43121693121693
2204	18	3.5396825396825378
2204	19	4.800264550264551
2204	20	3.919312169312157
2204	21	3.5238095238095255
2204	22	3.5304232804232853
2204	23	-8.429894179894184
2204	24	-0.11772486772486701
2204	25	3.845238095238095
2204	26	4.753968253968253
2204	27	3.497354497354493
2204	28	2.629629629629626
2204	29	3.7037037037037095
2204	30	4.3769841269841265
2204	31	4.996031746031747
2204	32	4.199735449735442
2204	33	5.527777777777779
2204	34	3.8068783068783034
2204	35	3.8875661375661323
2204	36	3.921957671957685
2204	37	5.084656084656082
2204	38	5.23677248677248
2204	39	5.93121693121693
2204	40	5.490740740740748
2204	41	4.9973544973545
2204	42	5.56613756613757
2204	43	4.904761904761905
2204	44	5.228835978835981
2204	45	3.6970899470899496
2204	46	4.2407407407407405
2204	47	5.283068783068778
2204	48	3.9682539682539684
2204	49	4.0383597883597915
2204	50	5.301587301587304
2204	51	4.033068783068792
2205	1	-0.9404761904761898
2205	2	2.179894179894184
2205	3	1.154761904761905
2205	4	-1.2962962962962905
2205	5	3.646825396825399
2205	6	2.603174603174601
2205	7	-2.8386243386243493
2205	8	0.4140211640211575
2205	9	-0.5224867724867792
2205	10	2.107142857142854
2205	11	2.797619047619051
2205	12	2.011904761904759
2205	13	2.7896825396825378
2205	14	-1.5145502645502589
2205	15	-6.661375661375665
2205	16	-11.939153439153444
2205	17	-0.3187830687830697
2205	18	1.5396825396825378
2205	19	-3.4497354497354493
2205	20	-2.330687830687843
2205	21	-6.4761904761904745
2205	22	2.5304232804232853
2205	23	-3.179894179894184
2205	24	-0.367724867724867
2205	25	-2.404761904761905
2205	26	1.003968253968253
2205	27	0.7473544973544932
2205	28	-3.120370370370374
2205	29	-4.0462962962962905
2205	30	0.6269841269841265
2205	31	1.496031746031747
2205	32	-1.0502645502645578
2205	33	2.5277777777777786
2205	34	1.8068783068783034
2205	35	-0.11243386243386766
2205	36	2.921957671957685
2205	37	3.084656084656082
2205	38	3.2367724867724803
2205	39	2.4312169312169303
2205	40	1.9907407407407476
2205	41	3.4973544973545003
2205	42	4.06613756613757
2205	43	1.404761904761905
2205	44	4.228835978835981
2205	45	1.6970899470899496
2205	46	3.7407407407407405
2205	47	0.2830687830687779
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1208	41	-2.5026455026454997
1208	42	3.56613756613757
1208	43	3.904761904761905
1208	44	5.228835978835981
1208	45	3.6970899470899496
1208	46	4.2407407407407405
1208	47	1.283068783068778
1208	48	4.968253968253968
1208	49	4.0383597883597915
1208	50	4.301587301587304
1208	51	5.033068783068792
1209	1	2.30952380952381
1209	2	-8.820105820105816
1209	3	4.154761904761905
1209	4	0.2037037037037095
1209	5	3.646825396825399
1209	6	2.103174603174601
1209	7	0.16137566137565074
1209	8	0.9140211640211575
1209	9	3.477513227513221
1209	10	3.107142857142854
1209	11	-3.202380952380949
1209	12	4.011904761904759
1209	13	3.7896825396825378
1209	14	3.985449735449741
1209	15	3.588624338624335
1209	16	0.8108465608465565
1209	17	-8.06878306878307
1209	18	3.5396825396825378
1209	19	3.8002645502645507
1209	20	1.9193121693121569
1209	21	2.5238095238095255
1209	22	3.5304232804232853
1209	23	4.070105820105816
1209	24	-9.617724867724867
1209	25	1.845238095238095
1209	26	-0.7460317460317469
1209	27	1.4973544973544932
1209	28	3.629629629629626
1209	29	1.7037037037037095
1209	30	4.3769841269841265
1209	31	0.9960317460317469
1209	32	1.1997354497354422
1209	33	-7.972222222222221
1209	34	3.8068783068783034
1209	35	3.8875661375661323
1209	36	0.921957671957685
1209	37	4.084656084656082
1209	38	-1.2632275132275197
1209	39	1.9312169312169303
1209	40	4.490740740740748
1209	41	-8.5026455026455
1209	42	-7.93386243386243
1209	43	-8.595238095238095
1209	44	5.228835978835981
1209	45	3.6970899470899496
1209	46	4.2407407407407405
1209	47	4.283068783068778
1209	48	4.968253968253968
1209	49	1.0383597883597915
1209	50	4.301587301587304
1209	51	6.033068783068792
1214	1	-10.19047619047619
1214	2	3.679894179894184
1214	3	-1.845238095238095
1214	4	-2.7962962962962905
1214	5	-11.853174603174601
1214	6	-0.896825396825399
1214	7	-3.8386243386243493
1214	8	-3.0859788359788425
1214	9	-10.02248677248678
1214	10	-9.392857142857146
1214	11	2.297619047619051
1214	12	3.011904761904759
1214	13	-0.21031746031746223
1214	14	3.985449735449741
1214	15	0.588624338624335
1214	16	3.8108465608465565
1214	17	-8.06878306878307
1214	18	-8.960317460317462
1214	19	-9.69973544973545
1214	20	-1.0806878306878431
1214	21	-0.4761904761904745
1214	22	2.5304232804232853
1214	23	0.07010582010581601
1214	24	-1.117724867724867
1214	25	0.8452380952380949
1214	26	-2.246031746031747
1214	27	0.4973544973544932
1214	28	-2.870370370370374
1214	29	3.7037037037037095
1214	30	-5.1230158730158735
1214	31	-9.503968253968253
1214	32	-9.300264550264558
1214	33	-1.9722222222222214
1214	34	-2.6931216931216966
1214	35	-8.612433862433868
1214	36	-9.578042328042315
1214	37	-9.415343915343918
1214	38	-2.2632275132275197
1214	39	3.9312169312169303
1214	40	3.4907407407407476
1214	41	-0.0026455026454996755
1214	42	1.56613756613757
1214	43	-12.595238095238095
1214	44	-13.771164021164019
1214	45	-8.30291005291005
1214	46	-21.75925925925926
1214	47	-5.216931216931222
1214	48	-1.5317460317460316
1214	49	-2.4616402116402085
1214	50	-2.198412698412696
1214	51	-13.966931216931208
1215	1	5.30952380952381
1215	2	0.679894179894184
1215	3	-8.345238095238095
1215	4	-2.7962962962962905
1215	5	-7.353174603174601
1215	6	-0.896825396825399
1215	7	2.1613756613756507
1215	8	-3.0859788359788425
1215	9	-0.5224867724867792
1215	10	-9.392857142857146
1215	11	-13.202380952380949
1215	12	-9.48809523809524
1215	13	-5.710317460317462
1215	14	-2.514550264550259
1215	15	-5.911375661375665
1215	16	-8.189153439153444
1215	17	-2.0687830687830697
1215	18	-0.46031746031746223
1215	19	-3.1997354497354493
1215	20	-23.080687830687843
1215	21	-3.4761904761904745
1215	22	2.5304232804232853
1215	23	3.070105820105816
1215	24	1.882275132275133
1215	25	0.8452380952380949
1215	26	4.753968253968253
1215	27	4.497354497354493
1215	28	3.629629629629626
1215	29	3.7037037037037095
1215	30	1.3769841269841265
1215	31	-9.503968253968253
1215	32	-2.800264550264558
1215	33	0.5277777777777786
1215	34	-9.693121693121697
1215	35	-9.612433862433868
1215	36	-9.578042328042315
1215	37	-2.415343915343918
1215	38	-9.26322751322752
1215	39	-1.5687830687830697
1215	40	0.4907407407407476
1215	41	-0.0026455026454996755
1215	42	-2.43386243386243
1215	43	2.904761904761905
1215	44	4.228835978835981
1215	45	-3.3029100529100504
1215	46	-9.25925925925926
1215	47	-9.216931216931222
1215	48	-1.5317460317460316
1215	49	0.03835978835979148
1215	50	3.301587301587304
1215	51	2.033068783068792
1212	1	2.30952380952381
1212	2	0.679894179894184
1212	3	4.154761904761905
1212	4	3.2037037037037095
1212	5	0.646825396825399
1212	6	0.10317460317460103
1212	7	-9.33862433862435
1212	8	-2.5859788359788425
1212	9	2.477513227513221
1212	10	3.107142857142854
1212	11	2.297619047619051
1212	12	-2.488095238095241
1212	13	-1.7103174603174622
1212	14	-3.014550264550259
1212	15	-12.911375661375665
1212	16	-2.6891534391534435
1212	17	0.4312169312169303
1212	18	-9.960317460317462
1212	19	2.8002645502645507
1212	20	-1.0806878306878431
1212	21	2.5238095238095255
1212	22	-12.969576719576715
1212	23	-2.429894179894184
1212	24	-1.117724867724867
1212	25	-8.654761904761905
1212	26	-8.746031746031747
1212	27	3.497354497354493
1212	28	-0.3703703703703738
1212	29	-8.79629629629629
1212	30	0.37698412698412653
1212	31	-9.503968253968253
1212	32	-9.300264550264558
1212	33	-8.972222222222221
1212	34	-2.6931216931216966
1212	35	-2.6124338624338677
1212	36	-9.578042328042315
1212	37	-9.415343915343918
1212	38	-2.2632275132275197
1212	39	-0.5687830687830697
1212	40	1.4907407407407476
1212	41	-0.0026455026454996755
1212	42	-7.93386243386243
1212	43	3.904761904761905
1212	44	-1.7711640211640187
1212	45	-2.8029100529100504
1212	46	-9.25925925925926
1212	47	-9.216931216931222
1212	48	-8.531746031746032
1212	49	-2.4616402116402085
1212	50	3.301587301587304
1212	51	4.033068783068792
1213	1	-19.69047619047619
1213	2	-7.320105820105816
1213	3	-20.845238095238095
1213	4	-7.7962962962962905
1213	5	3.646825396825399
1213	6	2.103174603174601
1213	7	2.1613756613756507
1213	8	-8.085978835978842
1213	9	-8.52248677248678
1213	10	3.107142857142854
1213	11	2.297619047619051
1213	12	-2.988095238095241
1213	13	-22.210317460317462
1213	14	-8.014550264550259
1213	15	-22.411375661375665
1213	16	-8.189153439153444
1213	17	-2.5687830687830697
1213	18	-8.460317460317462
1213	19	-3.1997354497354493
1213	20	1.9193121693121569
1213	21	-3.4761904761904745
1213	22	2.5304232804232853
1213	23	-2.929894179894184
1213	24	1.882275132275133
1213	25	-2.154761904761905
1213	26	-2.246031746031747
1213	27	3.497354497354493
1213	28	-3.370370370370374
1213	29	-21.29629629629629
1213	30	-7.6230158730158735
1213	31	-3.003968253968253
1213	32	-21.800264550264558
1213	33	-2.4722222222222214
1213	34	-8.193121693121697
1213	35	-22.112433862433868
1213	36	-8.078042328042315
1213	37	-2.915343915343918
1213	38	-7.76322751322752
1213	39	-21.06878306878307
1213	40	-21.509259259259252
1213	41	2.9973544973545003
1213	42	3.56613756613757
1213	43	2.904761904761905
1213	44	-20.77116402116402
1213	45	-8.30291005291005
1213	46	-2.7592592592592595
1213	47	-21.716931216931222
1213	48	-21.03174603174603
1213	49	-7.9616402116402085
1213	50	-21.698412698412696
1213	51	-20.966931216931208
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
22	2.0
23	6.0
24	8.0
25	10.0
26	21.0
27	29.0
28	50.0
29	46.0
30	73.0
31	75.0
32	85.0
33	100.0
34	115.0
35	133.0
36	135.0
37	203.0
38	260.0
39	232.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	27.79532533164877	12.823752368919772	11.054958938723942	48.32596336070752
2	10.549589387239418	16.740366392924827	52.179406190777	20.53063802905875
3	15.097915350600127	19.077700568540745	43.52495262160455	22.299431459254578
4	10.296904611497157	32.469993682880606	29.62728995578016	27.605811749842076
5	10.80227416298168	29.058749210360073	48.45230574857865	11.686670878079596
6	23.752368919772586	49.33670246367656	13.455464308275427	13.455464308275427
7	20.846493998736577	25.963360707517374	17.561591914087177	35.628553379658875
8	18.951358180669615	49.0840176879343	15.350600126342387	16.614024005053697
9	19.267214150347442	15.413771320277952	16.803537586860394	48.51547694251421
10	13.897662665824384	40.61907770056854	26.026531901452937	19.45672773215414
11	24.257738471257106	19.01452937460518	39.10296904611497	17.62476310802274
12	17.62476310802274	20.025268477574226	26.2792166771952	36.070751737207836
13	21.73089071383449	16.171825647504736	42.387871130764374	19.7094125078964
14	16.866708780795957	17.751105495893874	21.73089071383449	43.651295009475675
15	18.88818698673405	21.857233101705624	20.65698041692988	38.597599494630444
16	36.828806064434616	23.626026531901452	20.025268477574226	19.519898926089706
17	18.382817435249528	41.124447252053066	22.931143398610235	17.561591914087177
18	38.344914718888184	21.920404295641188	21.794061907770057	17.940619077700568
19	19.204042956411875	20.846493998736577	22.109917877447884	37.839545167403664
20	18.76184459886292	20.151610865445356	21.73089071383449	39.35565382185723
21	17.119393556538217	21.794061907770057	22.615287428932408	38.47125710675932
22	18.003790271636134	40.934933670246366	20.90966519267214	20.151610865445356
23	20.025268477574226	20.593809222994313	21.036007580543274	38.344914718888184
24	37.9658875552748	21.667719519898927	21.60454832596336	18.76184459886292
25	20.467466835123183	38.91345546430828	22.42577384712571	18.19330385344283
26	38.597599494630444	23.183828174352495	21.415034744156664	16.803537586860394
27	16.929879974731524	41.0612760581175	22.804801010739105	19.204042956411875
28	19.01452937460518	21.541377132027794	21.73089071383449	37.71320277953254
29	27.03727100442198	27.416298168035375	26.468730259001894	19.077700568540745
30	19.140871762476312	32.027795325331645	29.69046114971573	19.140871762476312
31	21.920404295641188	27.858496525584332	25.71067593177511	24.51042324699937
32	25.39481996209728	26.658243840808595	26.658243840808595	21.288692356285534
33	19.646241313960832	27.479469361970942	29.69046114971573	23.183828174352495
34	24.6367656348705	21.983575489576754	25.521162349968414	27.858496525584332
35	21.099178774478837	28.74289324068225	23.05748578648136	27.10044219835755
36	16.04548325963361	30.385344283006948	34.74415666456096	18.82501579279848
37	16.866708780795957	38.85028427037271	24.19456727732154	20.088439671509793
38	14.150347441566646	20.65698041692988	42.893240682248894	22.299431459254578
39	14.90840176879343	20.53063802905875	26.0897030953885	38.47125710675932
40	15.666456096020214	18.509159823120658	45.35691724573594	20.467466835123183
41	16.487681617182563	17.814276689829438	27.226784586228682	38.47125710675932
42	34.61781427668983	17.687934301958308	28.806064434617813	18.88818698673405
43	15.855969677826911	19.45672773215414	43.77763739734681	20.90966519267214
44	14.276689829437778	39.16614024005053	26.152874289324068	20.40429564118762
45	15.287428932406822	19.45672773215414	45.35691724573594	19.898926089703096
46	31.269740998104865	20.151610865445356	26.53190145293746	22.046746683512318
47	13.1396083385976	19.96209728363866	27.226784586228682	39.67150979153506
48	14.276689829437778	19.45672773215414	26.2792166771952	39.98736576121289
49	30.890713834491475	19.7094125078964	27.226784586228682	22.17308907138345
50	11.433986102337334	20.53063802905875	44.21983575489577	23.81554011370815
51	12.128869235628553	19.33038534428301	26.847757422615288	41.69298799747315
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.5
12	1.0
13	0.5
14	0.0
15	0.0
16	0.0
17	1.0
18	2.0
19	3.0
20	4.0
21	2.5
22	1.0
23	1.5
24	2.0
25	1.0
26	0.5
27	1.0
28	6.0
29	11.0
30	11.5
31	12.0
32	24.0
33	36.0
34	40.0
35	44.0
36	54.5
37	65.0
38	74.5
39	84.0
40	97.0
41	110.0
42	122.0
43	134.0
44	133.0
45	132.0
46	144.0
47	156.0
48	180.0
49	204.0
50	227.5
51	251.0
52	222.5
53	194.0
54	128.5
55	63.0
56	46.0
57	29.0
58	24.5
59	20.0
60	15.0
61	10.0
62	8.5
63	7.0
64	5.5
65	4.0
66	3.0
67	2.0
68	1.5
69	1.0
70	1.0
71	1.0
72	1.0
73	1.0
74	1.0
75	0.5
76	0.0
77	0.0
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1583.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	81.9330385344283
#Duplication Level	Percentage of deduplicated	Percentage of total
1	97.9182729375482	80.22741629816804
2	0.6168080185042405	1.010739102969046
3	0.30840400925212025	0.7580543272267846
4	0.2313030069390902	0.7580543272267846
5	0.15420200462606012	0.6317119393556538
6	0.07710100231303006	0.3790271636133923
7	0.07710100231303006	0.4421983575489577
8	0.0	0.0
9	0.0	0.0
>10	0.5397070161912104	12.065698041692988
>50	0.07710100231303006	3.7271004421983576
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACATAGACAAATCTCGTATGCCGTC	59	3.7271004421983576	No Hit
CTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGTC	45	2.8427037271004423	No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGTC	38	2.4005053695514844	No Hit
CTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGTC	35	2.2109917877447884	No Hit
CTTATACACATCTCCGAGCCCACGAGACGAATTGCTATCTCGTATGCCGTC	21	1.3265950726468732	RNA PCR Primer, Index 27 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGTC	20	1.2634238787113077	No Hit
CTTATACACATCTCCGAGCCCACGAGACGTCTTAGTATCTCGTATGCCGTC	19	1.2002526847757422	No Hit
CTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGTC	13	0.82122552116235	No Hit
CTTATACACATCTCCGAGCCCACGAGACAATGCGTTATCTCGTATGCCGTC	7	0.4421983575489577	No Hit
CTTATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGTC	6	0.3790271636133923	TruSeq Adapter, Index 12 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGTC	5	0.3158559696778269	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATAGACAAATCTCGTATGCCG	5	0.3158559696778269	No Hit
CTTATACACATCTCCGAGCCCACGAGACCTATTCAAATCTCGTATGCCGTC	4	0.2526847757422615	RNA PCR Primer, Index 13 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACATAGACAAATCTCGTATGCCGT	4	0.2526847757422615	No Hit
TTATACACATCTCCGAGCCCACGAGACGAATTGCTATCTCGTATGCCGTCT	4	0.2526847757422615	RNA PCR Primer, Index 38 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACTGATTACAATCTCGTATGCCGTC	3	0.18951358180669614	No Hit
TTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGTCT	3	0.18951358180669614	Illumina PCR Primer Index 8 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGT	3	0.18951358180669614	No Hit
TTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGTCT	3	0.18951358180669614	No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTAACCTATCTCGTATGCCGTC	2	0.12634238787113075	No Hit
TTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGTCT	2	0.12634238787113075	RNA PCR Primer, Index 27 (95% over 21bp)
TTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGTCT	2	0.12634238787113075	RNA PCR Primer, Index 44 (95% over 22bp)
ATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGTCTTC	2	0.12634238787113075	Illumina PCR Primer Index 4 (95% over 22bp)
CTCTACTACTATCATCAACATTCCTATGGATCCGAGCATCTTATCCACGCT	2	0.12634238787113075	No Hit
CTTATACACATCTCCGAGCCCACGAGACCTTGCAGAATCTCGTATGCCGTC	2	0.12634238787113075	No Hit
CTTATACACATCTCCGAGCCCACGAGACCATTCCGAATCTCGTATGCCGTC	2	0.12634238787113075	RNA PCR Primer, Index 46 (95% over 21bp)
GGTATCAACGCAGAGTACGGGGTGGTATCAACGCCTGTCTCTTATACACAT	2	0.12634238787113075	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA Adapter	Nextera Transposase Sequence
1	0.0	0.0	0.0
2	0.0	0.0	0.0
3	0.0	0.0	0.0
4	0.0	0.0	0.0
5	0.0	0.0	0.0
6	0.0	0.0	0.06317119393556538
7	0.0	0.0	0.06317119393556538
8	0.0	0.0	0.06317119393556538
9	0.0	0.0	0.06317119393556538
10	0.0	0.0	0.06317119393556538
11	0.0	0.0	0.06317119393556538
12	0.0	0.0	0.06317119393556538
13	0.0	0.0	0.06317119393556538
14	0.0	0.0	0.06317119393556538
15	0.0	0.0	0.06317119393556538
16	0.0	0.0	0.06317119393556538
17	0.0	0.0	0.06317119393556538
18	0.0	0.0	0.06317119393556538
19	0.0	0.0	0.06317119393556538
20	0.0	0.0	0.06317119393556538
21	0.0	0.0	0.12634238787113075
22	0.0	0.0	0.12634238787113075
23	0.0	0.0	0.18951358180669614
24	0.0	0.0	0.2526847757422615
25	0.0	0.0	0.2526847757422615
26	0.0	0.0	0.3158559696778269
27	0.0	0.0	0.4421983575489577
28	0.0	0.0	0.7580543272267846
29	0.0	0.0	0.9475679090334808
30	0.0	0.0	1.137081490840177
31	0.0	0.0	2.5268477574226154
32	0.0	0.0	3.979785217940619
33	0.0	0.0	4.990524320909665
34	0.0	0.0	5.938092229943146
35	0.0	0.0	7.643714466203411
36	0.0	0.0	8.717624763108022
37	0.0	0.0	10.423246999368288
38	0.0	0.0	12.760581174984207
39	0.0	0.0	15.097915350600127
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AATCTCG	20	5.0708407E-4	45.000004	36
CCACGAG	45	5.627953E-9	40.0	20
CACGAGA	45	5.627953E-9	40.0	21
ATCTCGT	40	1.3444333E-7	39.375004	37
ATCTCCG	40	1.3444333E-7	39.375004	10
TGCCGTC	40	1.3444333E-7	39.375004	45
TATGCCG	40	1.3444333E-7	39.375004	43
CATCTCC	40	1.3444333E-7	39.375004	9
ACATCTC	40	1.3444333E-7	39.375004	8
TCTCGTA	40	1.3444333E-7	39.375004	38
ACGAGAC	40	1.3444333E-7	39.375004	22
ATGCCGT	40	1.3444333E-7	39.375004	44
TCGTATG	40	1.3444333E-7	39.375004	40
TCTCCGA	40	1.3444333E-7	39.375004	11
CTCGTAT	40	1.3444333E-7	39.375004	39
CTCCGAG	40	1.3444333E-7	39.375004	12
AGCCCAC	35	3.1055934E-6	38.571426	17
GCCCACG	35	3.1055934E-6	38.571426	18
GAGCCCA	35	3.1055934E-6	38.571426	16
CCCACGA	35	3.1055934E-6	38.571426	19
>>END_MODULE