FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 30h_29.34100000005724.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 30h_29.34100000005724.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1583
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATAGACAAATCTCGTATGCCGTC593.7271004421983576No Hit
CTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGTC452.8427037271004423No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGTC382.4005053695514844No Hit
CTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGTC352.2109917877447884No Hit
CTTATACACATCTCCGAGCCCACGAGACGAATTGCTATCTCGTATGCCGTC211.3265950726468732RNA PCR Primer, Index 27 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGTC201.2634238787113077No Hit
CTTATACACATCTCCGAGCCCACGAGACGTCTTAGTATCTCGTATGCCGTC191.2002526847757422No Hit
CTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGTC130.82122552116235No Hit
CTTATACACATCTCCGAGCCCACGAGACAATGCGTTATCTCGTATGCCGTC70.4421983575489577No Hit
CTTATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGTC60.3790271636133923TruSeq Adapter, Index 12 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGTC50.3158559696778269No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATAGACAAATCTCGTATGCCG50.3158559696778269No Hit
CTTATACACATCTCCGAGCCCACGAGACCTATTCAAATCTCGTATGCCGTC40.2526847757422615RNA PCR Primer, Index 13 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACATAGACAAATCTCGTATGCCGT40.2526847757422615No Hit
TTATACACATCTCCGAGCCCACGAGACGAATTGCTATCTCGTATGCCGTCT40.2526847757422615RNA PCR Primer, Index 38 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACTGATTACAATCTCGTATGCCGTC30.18951358180669614No Hit
TTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGTCT30.18951358180669614Illumina PCR Primer Index 8 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGT30.18951358180669614No Hit
TTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGTCT30.18951358180669614No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTAACCTATCTCGTATGCCGTC20.12634238787113075No Hit
TTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGTCT20.12634238787113075RNA PCR Primer, Index 27 (95% over 21bp)
TTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGTCT20.12634238787113075RNA PCR Primer, Index 44 (95% over 22bp)
ATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGTCTTC20.12634238787113075Illumina PCR Primer Index 4 (95% over 22bp)
CTCTACTACTATCATCAACATTCCTATGGATCCGAGCATCTTATCCACGCT20.12634238787113075No Hit
CTTATACACATCTCCGAGCCCACGAGACCTTGCAGAATCTCGTATGCCGTC20.12634238787113075No Hit
CTTATACACATCTCCGAGCCCACGAGACCATTCCGAATCTCGTATGCCGTC20.12634238787113075RNA PCR Primer, Index 46 (95% over 21bp)
GGTATCAACGCAGAGTACGGGGTGGTATCAACGCCTGTCTCTTATACACAT20.12634238787113075No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCTCG205.0708407E-445.00000436
CCACGAG455.627953E-940.020
CACGAGA455.627953E-940.021
ATCTCGT401.3444333E-739.37500437
ATCTCCG401.3444333E-739.37500410
TGCCGTC401.3444333E-739.37500445
TATGCCG401.3444333E-739.37500443
CATCTCC401.3444333E-739.3750049
ACATCTC401.3444333E-739.3750048
TCTCGTA401.3444333E-739.37500438
ACGAGAC401.3444333E-739.37500422
ATGCCGT401.3444333E-739.37500444
TCGTATG401.3444333E-739.37500440
TCTCCGA401.3444333E-739.37500411
CTCGTAT401.3444333E-739.37500439
CTCCGAG401.3444333E-739.37500412
AGCCCAC353.1055934E-638.57142617
GCCCACG353.1055934E-638.57142618
GAGCCCA353.1055934E-638.57142616
CCCACGA353.1055934E-638.57142619
Produced by FastQC (version 0.11.2)