FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 30h_28.34100000005717.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 30h_28.34100000005717.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences492805
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA7850.027.5263521
GGTATCA3250.026.3176611
TACACTA450.001225028725.001865
TCTCGTA551.3669053E-424.5447938
GTCTTAA500.00225781122.5085281
CAGAACG500.002263143722.4993924
TGGCGCC500.002263143722.4993924
TGTATGA500.002263143722.4993922
TCTCCGA3650.021.5769545
CCCTAAG754.5823843E-521.0079571
TATCAAC10600.019.9522912
AACGCAG10700.019.557536
TCAACGC10750.019.464594
GTGTTAC706.9579546E-419.2930221
CTGCTAA1051.4369216E-619.2930221
CAACGCA10850.019.287155
ACGCAGA11000.019.2287047
CATCTCC14200.019.17396245
TATACTG600.006502547318.7513945
CTTCGCA600.00650635818.74949313
Produced by FastQC (version 0.11.2)