FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 30h_27.3410000000570a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 30h_27.3410000000570a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences260843
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTCA250.00210285336.0044488
ACGAGAC606.3955667E-933.74769622
CGTATGC651.3957106E-831.15172441
TATGCCG702.8701834E-828.92659843
TCGTATG755.6077624E-826.9981640
GCCCACG801.04602805E-725.3156318
CTCGTAT801.0479744E-725.31077439
CCCACGA801.0479744E-725.31077419
CCACGAG851.8832361E-723.82190720
GTATCAA5900.022.5070951
TAGAGAG500.002258330622.502785
CACGAGA903.2689422E-722.49846621
GACCTAT602.461556E-422.49846526
CGAGCCC955.491456E-721.31842215
CTATTCA654.228081E-420.76781729
GAACACT550.003925694620.4570736
CTAACCC550.003930058320.4531524
AGCCCAC1008.989264E-720.25250217
GTATGCC1009.005835E-720.2486242
TCTCGTA1009.005835E-720.2486238
Produced by FastQC (version 0.11.2)