FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 30h_26.341000000056fc.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 30h_26.341000000056fc.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences440213
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGACCCG300.00514156330.00272829
TCGTATG702.8783688E-828.92791440
TATGCCG856.4592314E-926.46998643
CGTATGC801.0509393E-725.31192641
CGAGCCC801.0509393E-725.31192615
CTACACG450.001225514624.9994344
GTATCAA6750.024.004911
GCCCACG952.1083906E-823.68367218
TAGGGGT500.002262705722.499494
TGCCGTC903.278128E-722.4994945
ATGCCGT1056.087794E-821.42808344
GACCTAT754.5911358E-521.00190726
GTCCTAT550.00392808320.4587291
CGAATGT550.003933256520.45408212
TCTCGTA1009.030846E-720.2495438
TCTCCGA4800.019.68705245
GACCGTT706.9751695E-419.28527611
CCCACGA1051.4417456E-619.28527619
ACCGTTC706.9751695E-419.28527612
AACGCAG7700.018.7008746
Produced by FastQC (version 0.11.2)