FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 30h_25.341000000056ef.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 30h_25.341000000056ef.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences326059
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATACCACCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGA9880.30301264495076047No Hit
GTATCAACGCAGAGTACGGGGTGGTATCCTGTCTCTTATACACATCTCCGA9130.28001067291502457No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAACTGTCTCTTATAC8370.25670200791881226No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAACTGTCTCTTATACA7090.21744530897782302No Hit
TTTTTGCGTTGATACCACCCCGTACTCTGCGTTGATACCTGTCTCTTATAC7060.2165252300963936No Hit
TGATACCACCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCG7000.21468507233353473No Hit
GATACCACCCCGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCG6630.20333743279590502No Hit
GGTATCAACGCAGAGTACGGGGTGGTATCCTGTCTCTTATACACATCTCCG6510.1996571172701873No Hit
GTATCAACGCAGAGTACGGGGTGGTATCACTGTCTCTTATACACATCTCCG6300.19321656510018126No Hit
TTTTTGCGTTGATACCACCCCGTACTCTGCGTTGATACTGTCTCTTATACA5840.17910868891826326No Hit
GATACCACCCCGTACTCTGCGTTGATACTGTCTCTTATACACATCTCCGAG5690.17450829451111607No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAACTGTCTCTTATA5530.16960120714349244No Hit
CTTATACACATCTCCGAGCCCACGAGACCTATTCAAATCTCGTATGCCGTC5470.16776104938063358RNA PCR Primer, Index 13 (95% over 21bp)
TATCAACGCAGAGTACGGGGTGGTATCCTGTCTCTTATACACATCTCCGAG5230.1604004183291981No Hit
TTTTTTGCGTTGATACCACCCCGTACTCTGCGTTGATACCTGTCTCTTATA4350.13341143780726802No Hit
GTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4280.13126458708393265No Hit
ATACCACTGCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCG3870.11869017570439706No Hit
TGATACCACCCCGTACTCTGCGTTGATACTGTCTCTTATACACATCTCCGA3860.11838348274392059No Hit
TATCAACGCAGAGTACGGGGTGGTATCACTGTCTCTTATACACATCTCCGA3850.1180767897834441No Hit
GAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3640.11163623761343806No Hit
GTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAACTGTCTCTTATACA3590.11010277281105567No Hit
GTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3520.10795592208772031No Hit
GTATCAACGCAGAGTACGGGCAGTGGTATCTGTCTCTTATACACATCTCCG3450.10580907136438497No Hit
CGTCTATGTGGCAAAATAGTGAGAAGATTTTTAGGTAGAGGTGAAAAGCCT3420.10488899248295552No Hit
TTTTTTGCGTTGATACCACCCCGTACTCTGCGTTGATACTGTCTCTTATAC3420.10488899248295552No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3300.1012086769572378No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC1000.038.24778745
GGGTAGT301.13748945E-437.50358224
TCTCGCA250.002105498235.99791721
AGTGTCA250.002105498235.99791721
CTCGTAT1050.034.2837339
GGACATA606.377377E-933.763581
ATATGTA401.5528698E-533.7635771
CGTATGC1150.033.2589441
TATGCCG1150.033.2589443
ATGCCGT1150.033.2589444
TCTCGTA1100.032.72537638
GTCTAGT352.8093357E-432.155791
ACAACTT352.8169336E-432.1409955
AATCTCG950.030.7876936
TCCGGAT300.00513951530.00286514
CCGGATG300.00513951530.00286515
ACCTATT1050.030.00286527
GGGGTCT300.00513951530.00286518
CCGTGTC300.00514337129.99826236
TCGTATG1400.028.92689740
Produced by FastQC (version 0.11.2)