FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 30h_23.341000000056c5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 30h_23.341000000056c5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences493631
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG708.112693E-1032.14169743
GGTATCA2400.029.0850241
GTATCAA5450.026.855661
TCTCGTA952.1100277E-823.68335738
TGCCGTC1052.3574103E-923.5729745
CTACTAA602.4518507E-422.5174391
TAACTAG500.002252628322.5174371
GACGTGC500.00226193522.5014696
TACTAAT702.69971E-522.499192
CCCACGA955.520469E-721.31502219
ATGCCGT1201.1017619E-820.62425644
CTCGTAT1109.958967E-820.45380839
TCCTATA550.003934226520.4538082
GGGACGA550.003934226520.45380836
ATCTCCG7050.020.10769745
ACGAGAC1251.7618731E-819.79928822
TACGGGG3150.019.2850215
TATCAAC7950.019.244592
GTGTAGG1302.7383066E-819.0532171
AACGCAG7850.018.9184326
Produced by FastQC (version 0.11.2)