FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 30h_22.341000000056b8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 30h_22.341000000056b8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences389402
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA2600.032.0305631
CTATTGT300.00513423530.0106161
GTATCAA6850.028.2581731
CTAATGT507.147983E-526.9991534
GCTTAAG500.002257294522.5079631
TCTACGG500.002262354822.49929427
CACCGAC602.4639704E-422.4992927
CCGAGTT550.00392972820.4565313
ATCTCCG5350.019.76827245
TATCAAC9900.019.3175752
CGAGTTC706.967572E-419.28758414
CGGGGGA1051.4392208E-619.28758417
AACAGCG706.973598E-419.2851097
CATCTCC13650.018.95788445
GGGTAGT600.006504126418.749416
TCGAGGA600.006504126418.7494119
TGTACCG600.006504126418.749415
TCAACGC10300.018.5673794
CAACGCA10500.018.2137135
ATCAACG10650.017.9571843
Produced by FastQC (version 0.11.2)